Hydrogen sulfide suppresses outward rectifier potassium currents in human pluripotent stem cell-derived cardiomyocytes.

<h4>Aim</h4>Hydrogen sulfide (H₂S) is a promising cardioprotective agent and a potential modulator of cardiac ion currents. Yet its cardiac effects on humans are poorly understood due to lack of functional cardiomyocytes. This study investigates electrophysiological responses of human pl...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Heming Wei, Guangqin Zhang, Suhua Qiu, Jun Lu, Jingwei Sheng, Manasi, Grace Tan, Philip Wong, Shu Uin Gan, Winston Shim
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b9311f0a0dfb44ec80e032ac26ee02d0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b9311f0a0dfb44ec80e032ac26ee02d0
record_format	dspace
spelling	oai:doaj.org-article:b9311f0a0dfb44ec80e032ac26ee02d02021-11-18T08:06:46ZHydrogen sulfide suppresses outward rectifier potassium currents in human pluripotent stem cell-derived cardiomyocytes.1932-620310.1371/journal.pone.0050641https://doaj.org/article/b9311f0a0dfb44ec80e032ac26ee02d02012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23226343/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Aim</h4>Hydrogen sulfide (H₂S) is a promising cardioprotective agent and a potential modulator of cardiac ion currents. Yet its cardiac effects on humans are poorly understood due to lack of functional cardiomyocytes. This study investigates electrophysiological responses of human pluripotent stem cells (hPSCs) derived cardiomyocytes towards H₂S.<h4>Methods and results</h4>Cardiomyocytes of ventricular, atrial and nodal subtypes differentiated from H9 embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were electrophysiologically characterized. The effect of NaHS, a donor of H₂S, on action potential (AP), outward rectifier potassium currents (I(Ks) and I(Kr)), L-type Ca²⁺ currents (I(CaL)) and hyperpolarization-activated inward current (I(f)) were determined by patch-clamp electrophysiology and confocal calcium imaging. In a concentration-dependent manner, NaHS (100 to 300 µM) consistently altered the action potential properties including prolonging action potential duration (APD) and slowing down contracting rates of ventricular-and atrial-like cardiomyocytes derived from both hESCs and hiPSCs. Moreover, inhibitions of slow and rapid I(K) (I(Ks) and I(Kr)), I(CaL) and I(f) were found in NaHS treated cardiomyocytes and it could collectively contribute to the remodeling of AP properties.<h4>Conclusions</h4>This is the first demonstration of effects of H₂S on cardiac electrophysiology of human ventricular-like, atrial-like and nodal-like cardiomyocytes. It reaffirmed the inhibitory effect of H₂S on I(CaL) and revealed additional novel inhibitory effects on I(f), I(Ks) and I(Kr) currents in human cardiomyocytes.Heming WeiGuangqin ZhangSuhua QiuJun LuJingwei ShengManasiGrace TanPhilip WongShu Uin GanWinston ShimPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e50641 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heming Wei Guangqin Zhang Suhua Qiu Jun Lu Jingwei Sheng Manasi Grace Tan Philip Wong Shu Uin Gan Winston Shim Hydrogen sulfide suppresses outward rectifier potassium currents in human pluripotent stem cell-derived cardiomyocytes.
description	<h4>Aim</h4>Hydrogen sulfide (H₂S) is a promising cardioprotective agent and a potential modulator of cardiac ion currents. Yet its cardiac effects on humans are poorly understood due to lack of functional cardiomyocytes. This study investigates electrophysiological responses of human pluripotent stem cells (hPSCs) derived cardiomyocytes towards H₂S.<h4>Methods and results</h4>Cardiomyocytes of ventricular, atrial and nodal subtypes differentiated from H9 embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were electrophysiologically characterized. The effect of NaHS, a donor of H₂S, on action potential (AP), outward rectifier potassium currents (I(Ks) and I(Kr)), L-type Ca²⁺ currents (I(CaL)) and hyperpolarization-activated inward current (I(f)) were determined by patch-clamp electrophysiology and confocal calcium imaging. In a concentration-dependent manner, NaHS (100 to 300 µM) consistently altered the action potential properties including prolonging action potential duration (APD) and slowing down contracting rates of ventricular-and atrial-like cardiomyocytes derived from both hESCs and hiPSCs. Moreover, inhibitions of slow and rapid I(K) (I(Ks) and I(Kr)), I(CaL) and I(f) were found in NaHS treated cardiomyocytes and it could collectively contribute to the remodeling of AP properties.<h4>Conclusions</h4>This is the first demonstration of effects of H₂S on cardiac electrophysiology of human ventricular-like, atrial-like and nodal-like cardiomyocytes. It reaffirmed the inhibitory effect of H₂S on I(CaL) and revealed additional novel inhibitory effects on I(f), I(Ks) and I(Kr) currents in human cardiomyocytes.
format	article
author	Heming Wei Guangqin Zhang Suhua Qiu Jun Lu Jingwei Sheng Manasi Grace Tan Philip Wong Shu Uin Gan Winston Shim
author_facet	Heming Wei Guangqin Zhang Suhua Qiu Jun Lu Jingwei Sheng Manasi Grace Tan Philip Wong Shu Uin Gan Winston Shim
author_sort	Heming Wei
title	Hydrogen sulfide suppresses outward rectifier potassium currents in human pluripotent stem cell-derived cardiomyocytes.
title_short	Hydrogen sulfide suppresses outward rectifier potassium currents in human pluripotent stem cell-derived cardiomyocytes.
title_full	Hydrogen sulfide suppresses outward rectifier potassium currents in human pluripotent stem cell-derived cardiomyocytes.
title_fullStr	Hydrogen sulfide suppresses outward rectifier potassium currents in human pluripotent stem cell-derived cardiomyocytes.
title_full_unstemmed	Hydrogen sulfide suppresses outward rectifier potassium currents in human pluripotent stem cell-derived cardiomyocytes.
title_sort	hydrogen sulfide suppresses outward rectifier potassium currents in human pluripotent stem cell-derived cardiomyocytes.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/b9311f0a0dfb44ec80e032ac26ee02d0
work_keys_str_mv	AT hemingwei hydrogensulfidesuppressesoutwardrectifierpotassiumcurrentsinhumanpluripotentstemcellderivedcardiomyocytes AT guangqinzhang hydrogensulfidesuppressesoutwardrectifierpotassiumcurrentsinhumanpluripotentstemcellderivedcardiomyocytes AT suhuaqiu hydrogensulfidesuppressesoutwardrectifierpotassiumcurrentsinhumanpluripotentstemcellderivedcardiomyocytes AT junlu hydrogensulfidesuppressesoutwardrectifierpotassiumcurrentsinhumanpluripotentstemcellderivedcardiomyocytes AT jingweisheng hydrogensulfidesuppressesoutwardrectifierpotassiumcurrentsinhumanpluripotentstemcellderivedcardiomyocytes AT manasi hydrogensulfidesuppressesoutwardrectifierpotassiumcurrentsinhumanpluripotentstemcellderivedcardiomyocytes AT gracetan hydrogensulfidesuppressesoutwardrectifierpotassiumcurrentsinhumanpluripotentstemcellderivedcardiomyocytes AT philipwong hydrogensulfidesuppressesoutwardrectifierpotassiumcurrentsinhumanpluripotentstemcellderivedcardiomyocytes AT shuuingan hydrogensulfidesuppressesoutwardrectifierpotassiumcurrentsinhumanpluripotentstemcellderivedcardiomyocytes AT winstonshim hydrogensulfidesuppressesoutwardrectifierpotassiumcurrentsinhumanpluripotentstemcellderivedcardiomyocytes
_version_	1718422253318701056

Hydrogen sulfide suppresses outward rectifier potassium currents in human pluripotent stem cell-derived cardiomyocytes.

Ejemplares similares