A dry immersion model of microgravity modulates platelet phenotype, miRNA signature, and circulating plasma protein biomarker profile

A dry immersion model of microgravity modulates platelet phenotype, miRNA signature, and circulating plasma protein biomarker profile

Abstract Ground based research modalities of microgravity have been proposed as innovative methods to investigate the aetiology of chronic age-related conditions such as cardiovascular disease. Dry Immersion (DI), has been effectively used to interrogate the sequelae of physical inactivity (PI) and...

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Autores principales: Laura Twomey, Nastassia Navasiolava, Adrien Robin, Marie-Pierre Bareille, Guillemette Gauquelin-Koch, Arnaud Beck, Françoise Larcher, Gerardene Meade-Murphy, Sinead Sheridan, Patricia B. Maguire, Michael Harrison, Bernard Degryse, Niall M. Moyna, Claude Gharib, Marc-Antoine Custaud, Ronan P. Murphy
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/b93d0c54ffb846f8add8113daf6fda14
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spelling oai:doaj.org-article:b93d0c54ffb846f8add8113daf6fda142021-11-14T12:18:01ZA dry immersion model of microgravity modulates platelet phenotype, miRNA signature, and circulating plasma protein biomarker profile10.1038/s41598-021-01335-x2045-2322https://doaj.org/article/b93d0c54ffb846f8add8113daf6fda142021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01335-xhttps://doaj.org/toc/2045-2322Abstract Ground based research modalities of microgravity have been proposed as innovative methods to investigate the aetiology of chronic age-related conditions such as cardiovascular disease. Dry Immersion (DI), has been effectively used to interrogate the sequelae of physical inactivity (PI) and microgravity on multiple physiological systems. Herein we look at the causa et effectus of 3-day DI on platelet phenotype, and correlate with both miRomic and circulating biomarker expression. The miRomic profile of platelets is reflective of phenotype, which itself is sensitive and malleable to the exposome, undergoing responsive transitions in order to fulfil platelets role in thrombosis and haemostasis. Heterogeneous platelet subpopulations circulate at any given time, with varying degrees of sensitivity to activation. Employing a DI model, we investigate the effect of acute PI on platelet function in 12 healthy males. 3-day DI resulted in a significant increase in platelet count, plateletcrit, platelet adhesion, aggregation, and a modest elevation of platelet reactivity index (PRI). We identified 15 protein biomarkers and 22 miRNA whose expression levels were altered after DI. A 3-day DI model of microgravity/physical inactivity induced a prothrombotic platelet phenotype with an unique platelet miRNA signature, increased platelet count and plateletcrit. This correlated with a unique circulating protein biomarker signature. Taken together, these findings highlight platelets as sensitive adaptive sentinels and functional biomarkers of epigenetic drift within the cardiovascular compartment.Laura TwomeyNastassia NavasiolavaAdrien RobinMarie-Pierre BareilleGuillemette Gauquelin-KochArnaud BeckFrançoise LarcherGerardene Meade-MurphySinead SheridanPatricia B. MaguireMichael HarrisonBernard DegryseNiall M. MoynaClaude GharibMarc-Antoine CustaudRonan P. MurphyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Laura Twomey
Nastassia Navasiolava
Adrien Robin
Marie-Pierre Bareille
Guillemette Gauquelin-Koch
Arnaud Beck
Françoise Larcher
Gerardene Meade-Murphy
Sinead Sheridan
Patricia B. Maguire
Michael Harrison
Bernard Degryse
Niall M. Moyna
Claude Gharib
Marc-Antoine Custaud
Ronan P. Murphy
A dry immersion model of microgravity modulates platelet phenotype, miRNA signature, and circulating plasma protein biomarker profile
description Abstract Ground based research modalities of microgravity have been proposed as innovative methods to investigate the aetiology of chronic age-related conditions such as cardiovascular disease. Dry Immersion (DI), has been effectively used to interrogate the sequelae of physical inactivity (PI) and microgravity on multiple physiological systems. Herein we look at the causa et effectus of 3-day DI on platelet phenotype, and correlate with both miRomic and circulating biomarker expression. The miRomic profile of platelets is reflective of phenotype, which itself is sensitive and malleable to the exposome, undergoing responsive transitions in order to fulfil platelets role in thrombosis and haemostasis. Heterogeneous platelet subpopulations circulate at any given time, with varying degrees of sensitivity to activation. Employing a DI model, we investigate the effect of acute PI on platelet function in 12 healthy males. 3-day DI resulted in a significant increase in platelet count, plateletcrit, platelet adhesion, aggregation, and a modest elevation of platelet reactivity index (PRI). We identified 15 protein biomarkers and 22 miRNA whose expression levels were altered after DI. A 3-day DI model of microgravity/physical inactivity induced a prothrombotic platelet phenotype with an unique platelet miRNA signature, increased platelet count and plateletcrit. This correlated with a unique circulating protein biomarker signature. Taken together, these findings highlight platelets as sensitive adaptive sentinels and functional biomarkers of epigenetic drift within the cardiovascular compartment.
format article
author Laura Twomey
Nastassia Navasiolava
Adrien Robin
Marie-Pierre Bareille
Guillemette Gauquelin-Koch
Arnaud Beck
Françoise Larcher
Gerardene Meade-Murphy
Sinead Sheridan
Patricia B. Maguire
Michael Harrison
Bernard Degryse
Niall M. Moyna
Claude Gharib
Marc-Antoine Custaud
Ronan P. Murphy
author_facet Laura Twomey
Nastassia Navasiolava
Adrien Robin
Marie-Pierre Bareille
Guillemette Gauquelin-Koch
Arnaud Beck
Françoise Larcher
Gerardene Meade-Murphy
Sinead Sheridan
Patricia B. Maguire
Michael Harrison
Bernard Degryse
Niall M. Moyna
Claude Gharib
Marc-Antoine Custaud
Ronan P. Murphy
author_sort Laura Twomey
title A dry immersion model of microgravity modulates platelet phenotype, miRNA signature, and circulating plasma protein biomarker profile
title_short A dry immersion model of microgravity modulates platelet phenotype, miRNA signature, and circulating plasma protein biomarker profile
title_full A dry immersion model of microgravity modulates platelet phenotype, miRNA signature, and circulating plasma protein biomarker profile
title_fullStr A dry immersion model of microgravity modulates platelet phenotype, miRNA signature, and circulating plasma protein biomarker profile
title_full_unstemmed A dry immersion model of microgravity modulates platelet phenotype, miRNA signature, and circulating plasma protein biomarker profile
title_sort dry immersion model of microgravity modulates platelet phenotype, mirna signature, and circulating plasma protein biomarker profile
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/b93d0c54ffb846f8add8113daf6fda14
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