Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing

Resistance of gRNA to ubiquitous ribonucleases is required for CRISPR-Cas9-based therapeutics. Here, the authors explore chemical modifications at all positions of the crRNA guide and tracrRNA cofactor, and identify modified versions that are more potent and stable than their unmodified counterparts...

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Main Authors:	Aamir Mir, Julia F. Alterman, Matthew R. Hassler, Alexandre J. Debacker, Edward Hudgens, Dimas Echeverria, Michael H. Brodsky, Anastasia Khvorova, Jonathan K. Watts, Erik J. Sontheimer
Format:	article
Language:	EN
Published:	Nature Portfolio 2018
Subjects:	Science Q
Online Access:	https://doaj.org/article/b945b146d35544ef9d5a4a30d633c3c0
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spelling	oai:doaj.org-article:b945b146d35544ef9d5a4a30d633c3c02021-12-02T14:40:05ZHeavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing10.1038/s41467-018-05073-z2041-1723https://doaj.org/article/b945b146d35544ef9d5a4a30d633c3c02018-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05073-zhttps://doaj.org/toc/2041-1723Resistance of gRNA to ubiquitous ribonucleases is required for CRISPR-Cas9-based therapeutics. Here, the authors explore chemical modifications at all positions of the crRNA guide and tracrRNA cofactor, and identify modified versions that are more potent and stable than their unmodified counterparts in editing human cells.Aamir MirJulia F. AltermanMatthew R. HasslerAlexandre J. DebackerEdward HudgensDimas EcheverriaMichael H. BrodskyAnastasia KhvorovaJonathan K. WattsErik J. SontheimerNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Aamir Mir Julia F. Alterman Matthew R. Hassler Alexandre J. Debacker Edward Hudgens Dimas Echeverria Michael H. Brodsky Anastasia Khvorova Jonathan K. Watts Erik J. Sontheimer Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
description	Resistance of gRNA to ubiquitous ribonucleases is required for CRISPR-Cas9-based therapeutics. Here, the authors explore chemical modifications at all positions of the crRNA guide and tracrRNA cofactor, and identify modified versions that are more potent and stable than their unmodified counterparts in editing human cells.
format	article
author	Aamir Mir Julia F. Alterman Matthew R. Hassler Alexandre J. Debacker Edward Hudgens Dimas Echeverria Michael H. Brodsky Anastasia Khvorova Jonathan K. Watts Erik J. Sontheimer
author_facet	Aamir Mir Julia F. Alterman Matthew R. Hassler Alexandre J. Debacker Edward Hudgens Dimas Echeverria Michael H. Brodsky Anastasia Khvorova Jonathan K. Watts Erik J. Sontheimer
author_sort	Aamir Mir
title	Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
title_short	Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
title_full	Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
title_fullStr	Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
title_full_unstemmed	Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
title_sort	heavily and fully modified rnas guide efficient spycas9-mediated genome editing
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b945b146d35544ef9d5a4a30d633c3c0
work_keys_str_mv	AT aamirmir heavilyandfullymodifiedrnasguideefficientspycas9mediatedgenomeediting AT juliafalterman heavilyandfullymodifiedrnasguideefficientspycas9mediatedgenomeediting AT matthewrhassler heavilyandfullymodifiedrnasguideefficientspycas9mediatedgenomeediting AT alexandrejdebacker heavilyandfullymodifiedrnasguideefficientspycas9mediatedgenomeediting AT edwardhudgens heavilyandfullymodifiedrnasguideefficientspycas9mediatedgenomeediting AT dimasecheverria heavilyandfullymodifiedrnasguideefficientspycas9mediatedgenomeediting AT michaelhbrodsky heavilyandfullymodifiedrnasguideefficientspycas9mediatedgenomeediting AT anastasiakhvorova heavilyandfullymodifiedrnasguideefficientspycas9mediatedgenomeediting AT jonathankwatts heavilyandfullymodifiedrnasguideefficientspycas9mediatedgenomeediting AT erikjsontheimer heavilyandfullymodifiedrnasguideefficientspycas9mediatedgenomeediting
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Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing

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