Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing

Resistance of gRNA to ubiquitous ribonucleases is required for CRISPR-Cas9-based therapeutics. Here, the authors explore chemical modifications at all positions of the crRNA guide and tracrRNA cofactor, and identify modified versions that are more potent and stable than their unmodified counterparts...

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Autores principales: Aamir Mir, Julia F. Alterman, Matthew R. Hassler, Alexandre J. Debacker, Edward Hudgens, Dimas Echeverria, Michael H. Brodsky, Anastasia Khvorova, Jonathan K. Watts, Erik J. Sontheimer
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Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/b945b146d35544ef9d5a4a30d633c3c0
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spelling oai:doaj.org-article:b945b146d35544ef9d5a4a30d633c3c02021-12-02T14:40:05ZHeavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing10.1038/s41467-018-05073-z2041-1723https://doaj.org/article/b945b146d35544ef9d5a4a30d633c3c02018-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05073-zhttps://doaj.org/toc/2041-1723Resistance of gRNA to ubiquitous ribonucleases is required for CRISPR-Cas9-based therapeutics. Here, the authors explore chemical modifications at all positions of the crRNA guide and tracrRNA cofactor, and identify modified versions that are more potent and stable than their unmodified counterparts in editing human cells.Aamir MirJulia F. AltermanMatthew R. HasslerAlexandre J. DebackerEdward HudgensDimas EcheverriaMichael H. BrodskyAnastasia KhvorovaJonathan K. WattsErik J. SontheimerNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Aamir Mir
Julia F. Alterman
Matthew R. Hassler
Alexandre J. Debacker
Edward Hudgens
Dimas Echeverria
Michael H. Brodsky
Anastasia Khvorova
Jonathan K. Watts
Erik J. Sontheimer
Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
description Resistance of gRNA to ubiquitous ribonucleases is required for CRISPR-Cas9-based therapeutics. Here, the authors explore chemical modifications at all positions of the crRNA guide and tracrRNA cofactor, and identify modified versions that are more potent and stable than their unmodified counterparts in editing human cells.
format article
author Aamir Mir
Julia F. Alterman
Matthew R. Hassler
Alexandre J. Debacker
Edward Hudgens
Dimas Echeverria
Michael H. Brodsky
Anastasia Khvorova
Jonathan K. Watts
Erik J. Sontheimer
author_facet Aamir Mir
Julia F. Alterman
Matthew R. Hassler
Alexandre J. Debacker
Edward Hudgens
Dimas Echeverria
Michael H. Brodsky
Anastasia Khvorova
Jonathan K. Watts
Erik J. Sontheimer
author_sort Aamir Mir
title Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
title_short Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
title_full Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
title_fullStr Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
title_full_unstemmed Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
title_sort heavily and fully modified rnas guide efficient spycas9-mediated genome editing
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/b945b146d35544ef9d5a4a30d633c3c0
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