Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
Resistance of gRNA to ubiquitous ribonucleases is required for CRISPR-Cas9-based therapeutics. Here, the authors explore chemical modifications at all positions of the crRNA guide and tracrRNA cofactor, and identify modified versions that are more potent and stable than their unmodified counterparts...
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Nature Portfolio
2018
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oai:doaj.org-article:b945b146d35544ef9d5a4a30d633c3c02021-12-02T14:40:05ZHeavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing10.1038/s41467-018-05073-z2041-1723https://doaj.org/article/b945b146d35544ef9d5a4a30d633c3c02018-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05073-zhttps://doaj.org/toc/2041-1723Resistance of gRNA to ubiquitous ribonucleases is required for CRISPR-Cas9-based therapeutics. Here, the authors explore chemical modifications at all positions of the crRNA guide and tracrRNA cofactor, and identify modified versions that are more potent and stable than their unmodified counterparts in editing human cells.Aamir MirJulia F. AltermanMatthew R. HasslerAlexandre J. DebackerEdward HudgensDimas EcheverriaMichael H. BrodskyAnastasia KhvorovaJonathan K. WattsErik J. SontheimerNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-9 (2018) |
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Science Q Aamir Mir Julia F. Alterman Matthew R. Hassler Alexandre J. Debacker Edward Hudgens Dimas Echeverria Michael H. Brodsky Anastasia Khvorova Jonathan K. Watts Erik J. Sontheimer Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing |
description |
Resistance of gRNA to ubiquitous ribonucleases is required for CRISPR-Cas9-based therapeutics. Here, the authors explore chemical modifications at all positions of the crRNA guide and tracrRNA cofactor, and identify modified versions that are more potent and stable than their unmodified counterparts in editing human cells. |
format |
article |
author |
Aamir Mir Julia F. Alterman Matthew R. Hassler Alexandre J. Debacker Edward Hudgens Dimas Echeverria Michael H. Brodsky Anastasia Khvorova Jonathan K. Watts Erik J. Sontheimer |
author_facet |
Aamir Mir Julia F. Alterman Matthew R. Hassler Alexandre J. Debacker Edward Hudgens Dimas Echeverria Michael H. Brodsky Anastasia Khvorova Jonathan K. Watts Erik J. Sontheimer |
author_sort |
Aamir Mir |
title |
Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing |
title_short |
Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing |
title_full |
Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing |
title_fullStr |
Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing |
title_full_unstemmed |
Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing |
title_sort |
heavily and fully modified rnas guide efficient spycas9-mediated genome editing |
publisher |
Nature Portfolio |
publishDate |
2018 |
url |
https://doaj.org/article/b945b146d35544ef9d5a4a30d633c3c0 |
work_keys_str_mv |
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