CYTOKINE PROFILE IN PATIENTS WITH CHRONIC VIRUS HEPATITIS COMPLICATED WITH FIBROSIS AND CIRRHOSIS

Abstract. In present study, a comparative analysis of spontaneous and LPS-stimulated production of Th1/ Th2 (IFN-γ/IL-4, IL-13), and monocyte-derived pro- and anti-inflammatory cytokines (TNF-α/IL-10) was carried out in blood cell cultures of healthy donors and the patients with chronic viral hepati...

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Autores principales:	E. R. Chernykh, N. M. Starostina, O. Yu. Leplina, E. Ya. Shevela, S. V. Agapitova, M. V. Shipunov, A. A. Ostanin, V. A. Kozlov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	th1/th2 anti-inflammatory cytokines proinflammatory cytokines liver cirrhosis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/b9490146732c45989e28d31e117e0f1d
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spelling	oai:doaj.org-article:b9490146732c45989e28d31e117e0f1d2021-11-18T08:03:42ZCYTOKINE PROFILE IN PATIENTS WITH CHRONIC VIRUS HEPATITIS COMPLICATED WITH FIBROSIS AND CIRRHOSIS1563-06252313-741X10.15789/1563-0625-2006-4-539-546https://doaj.org/article/b9490146732c45989e28d31e117e0f1d2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/490https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. In present study, a comparative analysis of spontaneous and LPS-stimulated production of Th1/ Th2 (IFN-γ/IL-4, IL-13), and monocyte-derived pro- and anti-inflammatory cytokines (TNF-α/IL-10) was carried out in blood cell cultures of healthy donors and the patients with chronic viral hepatitis complicated with liver fibrosis and cirrhosis. The patients’ cells exhibited an increased production of both Th1/proinflammatory and Th2/anti-inflammatory cytokines. Meanwhile, hepatitis-induced cirrhotic transformation correlates with enhancement of spontaneous IL-4 and IL-13 production (not observed in fibrosis patients), and progressive increase of spontaneous IFN-γ, TNF-α and IL-10 secretion. In turn, the progression of cirrhosis is connected with increase of pro-inflammatory blood activity and reduction of anti-inflammatory potential. E.g., the patients with grade C cirrhosis have significantly higher index of TNF-α/IL-10 and IFN-γ/IL-10 ratio, as compared to the grade A patients. Moreover, we revealed a positive correlation of TNF-α/IL-10 ratio with cytolysis and severity of cirrhosis (Child-Pugh score), and a reverse correlation between the grade of liver cirrhosis and LPS-induced stimulation index of IL-10. Predominance of pro-inflammatory cytokines, displayed as increased IFN-γ/IL-10 ratio, was also associated with presence of anemia. Hence, the profile of cytokines produced by peripheral blood cells reflects some clinical features in the patients with chronic virus hepatitis complicated with fibrosis and cirrhosis.E. R. ChernykhN. M. StarostinaO. Yu. LeplinaE. Ya. ShevelaS. V. AgapitovaM. V. ShipunovA. A. OstaninV. A. KozlovSPb RAACIarticleth1/th2anti-inflammatory cytokinesproinflammatory cytokinesliver cirrhosisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 8, Iss 4, Pp 539-546 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	th1/th2 anti-inflammatory cytokines proinflammatory cytokines liver cirrhosis Immunologic diseases. Allergy RC581-607
spellingShingle	th1/th2 anti-inflammatory cytokines proinflammatory cytokines liver cirrhosis Immunologic diseases. Allergy RC581-607 E. R. Chernykh N. M. Starostina O. Yu. Leplina E. Ya. Shevela S. V. Agapitova M. V. Shipunov A. A. Ostanin V. A. Kozlov CYTOKINE PROFILE IN PATIENTS WITH CHRONIC VIRUS HEPATITIS COMPLICATED WITH FIBROSIS AND CIRRHOSIS
description	Abstract. In present study, a comparative analysis of spontaneous and LPS-stimulated production of Th1/ Th2 (IFN-γ/IL-4, IL-13), and monocyte-derived pro- and anti-inflammatory cytokines (TNF-α/IL-10) was carried out in blood cell cultures of healthy donors and the patients with chronic viral hepatitis complicated with liver fibrosis and cirrhosis. The patients’ cells exhibited an increased production of both Th1/proinflammatory and Th2/anti-inflammatory cytokines. Meanwhile, hepatitis-induced cirrhotic transformation correlates with enhancement of spontaneous IL-4 and IL-13 production (not observed in fibrosis patients), and progressive increase of spontaneous IFN-γ, TNF-α and IL-10 secretion. In turn, the progression of cirrhosis is connected with increase of pro-inflammatory blood activity and reduction of anti-inflammatory potential. E.g., the patients with grade C cirrhosis have significantly higher index of TNF-α/IL-10 and IFN-γ/IL-10 ratio, as compared to the grade A patients. Moreover, we revealed a positive correlation of TNF-α/IL-10 ratio with cytolysis and severity of cirrhosis (Child-Pugh score), and a reverse correlation between the grade of liver cirrhosis and LPS-induced stimulation index of IL-10. Predominance of pro-inflammatory cytokines, displayed as increased IFN-γ/IL-10 ratio, was also associated with presence of anemia. Hence, the profile of cytokines produced by peripheral blood cells reflects some clinical features in the patients with chronic virus hepatitis complicated with fibrosis and cirrhosis.
format	article
author	E. R. Chernykh N. M. Starostina O. Yu. Leplina E. Ya. Shevela S. V. Agapitova M. V. Shipunov A. A. Ostanin V. A. Kozlov
author_facet	E. R. Chernykh N. M. Starostina O. Yu. Leplina E. Ya. Shevela S. V. Agapitova M. V. Shipunov A. A. Ostanin V. A. Kozlov
author_sort	E. R. Chernykh
title	CYTOKINE PROFILE IN PATIENTS WITH CHRONIC VIRUS HEPATITIS COMPLICATED WITH FIBROSIS AND CIRRHOSIS
title_short	CYTOKINE PROFILE IN PATIENTS WITH CHRONIC VIRUS HEPATITIS COMPLICATED WITH FIBROSIS AND CIRRHOSIS
title_full	CYTOKINE PROFILE IN PATIENTS WITH CHRONIC VIRUS HEPATITIS COMPLICATED WITH FIBROSIS AND CIRRHOSIS
title_fullStr	CYTOKINE PROFILE IN PATIENTS WITH CHRONIC VIRUS HEPATITIS COMPLICATED WITH FIBROSIS AND CIRRHOSIS
title_full_unstemmed	CYTOKINE PROFILE IN PATIENTS WITH CHRONIC VIRUS HEPATITIS COMPLICATED WITH FIBROSIS AND CIRRHOSIS
title_sort	cytokine profile in patients with chronic virus hepatitis complicated with fibrosis and cirrhosis
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/b9490146732c45989e28d31e117e0f1d
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CYTOKINE PROFILE IN PATIENTS WITH CHRONIC VIRUS HEPATITIS COMPLICATED WITH FIBROSIS AND CIRRHOSIS

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