Differential profiling of lacrimal cytokines in patients suffering from thyroid-associated orbitopathy

Abstract The aim was to investigate the levels of cytokines and soluble IL-6R in the tears of patients with thyroid-associated orbitopathy (TAO) disease. Schirmer’s test was adopted to collect tears from TAO patients (N = 20, 17 women, mean age (±SD): 46.0 years (±13.4)) and healthy subjects (N = 18...

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Autores principales:	Edina Kishazi, Marianne Dor, Simone Eperon, Aurélie Oberic, Natacha Turck, Mehrad Hamedani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b94ee87716ce4d418d71b67ec7aaf20d
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spelling	oai:doaj.org-article:b94ee87716ce4d418d71b67ec7aaf20d2021-12-02T15:09:02ZDifferential profiling of lacrimal cytokines in patients suffering from thyroid-associated orbitopathy10.1038/s41598-018-29113-22045-2322https://doaj.org/article/b94ee87716ce4d418d71b67ec7aaf20d2018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29113-2https://doaj.org/toc/2045-2322Abstract The aim was to investigate the levels of cytokines and soluble IL-6R in the tears of patients with thyroid-associated orbitopathy (TAO) disease. Schirmer’s test was adopted to collect tears from TAO patients (N = 20, 17 women, mean age (±SD): 46.0 years (±13.4)) and healthy subjects (N = 18, 10 women, 45.4 years (±18.7)). Lacrimal cytokines and soluble IL-6R (sIL-6R) were measured using a 10-plex panel (Meso Scale Discovery Company) and Invitrogen Human sIL-6R Elisa kit, respectively. Tear levels of IL-10, IL-12p70, IL-13, IL-6 and TNF-α appeared significantly higher in TAO patients than in healthy subjects. Interestingly, IL-10, IL-12p70 and IL-8 levels increased in tears whatever the form of TAO whereas IL-13, IL-6 and TNF-α levels were significantly elevated in inflammatory TAO patients, meaning with a clinical score activity (CAS) ≥ 3, compared to controls. Furthermore, only 3 cytokines were strongly positively correlated with CAS (IL-13 Spearman coeff. r: 0.703, p = 0.0005; IL-6 r: 0.553, p = 0.011; IL-8 r: 0.618, p = 0.004, respectively). Finally, tobacco use disturbed the levels of several cytokines, especially in patient suffering of TAO. The differential profile of lacrimal cytokines could be useful for the diagnosis of TAO patients. Nevertheless, the tobacco use of these patients should be taken into account in the interpretation of the cytokine levels.Edina KishaziMarianne DorSimone EperonAurélie ObericNatacha TurckMehrad HamedaniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Edina Kishazi Marianne Dor Simone Eperon Aurélie Oberic Natacha Turck Mehrad Hamedani Differential profiling of lacrimal cytokines in patients suffering from thyroid-associated orbitopathy
description	Abstract The aim was to investigate the levels of cytokines and soluble IL-6R in the tears of patients with thyroid-associated orbitopathy (TAO) disease. Schirmer’s test was adopted to collect tears from TAO patients (N = 20, 17 women, mean age (±SD): 46.0 years (±13.4)) and healthy subjects (N = 18, 10 women, 45.4 years (±18.7)). Lacrimal cytokines and soluble IL-6R (sIL-6R) were measured using a 10-plex panel (Meso Scale Discovery Company) and Invitrogen Human sIL-6R Elisa kit, respectively. Tear levels of IL-10, IL-12p70, IL-13, IL-6 and TNF-α appeared significantly higher in TAO patients than in healthy subjects. Interestingly, IL-10, IL-12p70 and IL-8 levels increased in tears whatever the form of TAO whereas IL-13, IL-6 and TNF-α levels were significantly elevated in inflammatory TAO patients, meaning with a clinical score activity (CAS) ≥ 3, compared to controls. Furthermore, only 3 cytokines were strongly positively correlated with CAS (IL-13 Spearman coeff. r: 0.703, p = 0.0005; IL-6 r: 0.553, p = 0.011; IL-8 r: 0.618, p = 0.004, respectively). Finally, tobacco use disturbed the levels of several cytokines, especially in patient suffering of TAO. The differential profile of lacrimal cytokines could be useful for the diagnosis of TAO patients. Nevertheless, the tobacco use of these patients should be taken into account in the interpretation of the cytokine levels.
format	article
author	Edina Kishazi Marianne Dor Simone Eperon Aurélie Oberic Natacha Turck Mehrad Hamedani
author_facet	Edina Kishazi Marianne Dor Simone Eperon Aurélie Oberic Natacha Turck Mehrad Hamedani
author_sort	Edina Kishazi
title	Differential profiling of lacrimal cytokines in patients suffering from thyroid-associated orbitopathy
title_short	Differential profiling of lacrimal cytokines in patients suffering from thyroid-associated orbitopathy
title_full	Differential profiling of lacrimal cytokines in patients suffering from thyroid-associated orbitopathy
title_fullStr	Differential profiling of lacrimal cytokines in patients suffering from thyroid-associated orbitopathy
title_full_unstemmed	Differential profiling of lacrimal cytokines in patients suffering from thyroid-associated orbitopathy
title_sort	differential profiling of lacrimal cytokines in patients suffering from thyroid-associated orbitopathy
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b94ee87716ce4d418d71b67ec7aaf20d
work_keys_str_mv	AT edinakishazi differentialprofilingoflacrimalcytokinesinpatientssufferingfromthyroidassociatedorbitopathy AT mariannedor differentialprofilingoflacrimalcytokinesinpatientssufferingfromthyroidassociatedorbitopathy AT simoneeperon differentialprofilingoflacrimalcytokinesinpatientssufferingfromthyroidassociatedorbitopathy AT aurelieoberic differentialprofilingoflacrimalcytokinesinpatientssufferingfromthyroidassociatedorbitopathy AT natachaturck differentialprofilingoflacrimalcytokinesinpatientssufferingfromthyroidassociatedorbitopathy AT mehradhamedani differentialprofilingoflacrimalcytokinesinpatientssufferingfromthyroidassociatedorbitopathy
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Differential profiling of lacrimal cytokines in patients suffering from thyroid-associated orbitopathy

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