Laboratory Activity to Teach about the Proliferation of <italic toggle="yes">Salmonella</italic> in Vegetables

We designed a three-week laboratory experience that can complement any microbiology teaching laboratory to expand students’ knowledge of the ecology of human enteric pathogens outside of their animal hosts. Through their participation in this laboratory activity, students learned that vegetative and...

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Autores principales:	Massimiliano Marvasi, Manika Choudhury, Max Teplitski
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2015
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b95b39ad21a64c8f8c13abb6a0ee1bff
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spelling	oai:doaj.org-article:b95b39ad21a64c8f8c13abb6a0ee1bff2021-11-15T15:04:04ZLaboratory Activity to Teach about the Proliferation of <italic toggle="yes">Salmonella</italic> in Vegetables10.1128/jmbe.v16i2.9481935-78851935-7877https://doaj.org/article/b95b39ad21a64c8f8c13abb6a0ee1bff2015-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v16i2.948https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885We designed a three-week laboratory experience that can complement any microbiology teaching laboratory to expand students’ knowledge of the ecology of human enteric pathogens outside of their animal hosts. Through their participation in this laboratory activity, students learned that vegetative and reproductive plant parts could be a natural habitat for enteric bacteria such as non-typhoidal strains of Salmonella enterica. This field was recently brought to the forefront of the scientific community and public interest by outbreaks of human illness linked to the consumption of fresh fruits and vegetables. Students were encouraged to develop their own testable hypotheses to compare proliferation of Salmonella enterica sv Typhimurium LT2 in different vegetables: cherry and regular-size tomatoes, onions, lettuce, and yellow and red bell peppers (Escherichia coli can be substituted for BSL1 laboratories). Upon completion of the laboratory experience, students were able to: 1) Develop testable hypotheses addressing the ability of a human pathogen, Salmonella enterica, to colonize and proliferate in vegetables; 2) Determine that different vegetables support the growth of Salmonella to different extents; 3) Conduct statistical analysis and identify any significant differences. The teaching-learning process was assessed with a pre-/posttest, with an average increase in content understanding from ~15% to 85%. We also measured students’ proficiency while conducting specific technical tasks, revealing no major difficulties while conducting the experiments. Students indicated satisfaction with the organization and content of the practices. All of the students (100%) agreed that the exercises improved their knowledge of this subject.Massimiliano MarvasiManika ChoudhuryMax TeplitskiAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 16, Iss 2, Pp 230-236 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Massimiliano Marvasi Manika Choudhury Max Teplitski Laboratory Activity to Teach about the Proliferation of <italic toggle="yes">Salmonella</italic> in Vegetables
description	We designed a three-week laboratory experience that can complement any microbiology teaching laboratory to expand students’ knowledge of the ecology of human enteric pathogens outside of their animal hosts. Through their participation in this laboratory activity, students learned that vegetative and reproductive plant parts could be a natural habitat for enteric bacteria such as non-typhoidal strains of Salmonella enterica. This field was recently brought to the forefront of the scientific community and public interest by outbreaks of human illness linked to the consumption of fresh fruits and vegetables. Students were encouraged to develop their own testable hypotheses to compare proliferation of Salmonella enterica sv Typhimurium LT2 in different vegetables: cherry and regular-size tomatoes, onions, lettuce, and yellow and red bell peppers (Escherichia coli can be substituted for BSL1 laboratories). Upon completion of the laboratory experience, students were able to: 1) Develop testable hypotheses addressing the ability of a human pathogen, Salmonella enterica, to colonize and proliferate in vegetables; 2) Determine that different vegetables support the growth of Salmonella to different extents; 3) Conduct statistical analysis and identify any significant differences. The teaching-learning process was assessed with a pre-/posttest, with an average increase in content understanding from ~15% to 85%. We also measured students’ proficiency while conducting specific technical tasks, revealing no major difficulties while conducting the experiments. Students indicated satisfaction with the organization and content of the practices. All of the students (100%) agreed that the exercises improved their knowledge of this subject.
format	article
author	Massimiliano Marvasi Manika Choudhury Max Teplitski
author_facet	Massimiliano Marvasi Manika Choudhury Max Teplitski
author_sort	Massimiliano Marvasi
title	Laboratory Activity to Teach about the Proliferation of <italic toggle="yes">Salmonella</italic> in Vegetables
title_short	Laboratory Activity to Teach about the Proliferation of <italic toggle="yes">Salmonella</italic> in Vegetables
title_full	Laboratory Activity to Teach about the Proliferation of <italic toggle="yes">Salmonella</italic> in Vegetables
title_fullStr	Laboratory Activity to Teach about the Proliferation of <italic toggle="yes">Salmonella</italic> in Vegetables
title_full_unstemmed	Laboratory Activity to Teach about the Proliferation of <italic toggle="yes">Salmonella</italic> in Vegetables
title_sort	laboratory activity to teach about the proliferation of <italic toggle="yes">salmonella</italic> in vegetables
publisher	American Society for Microbiology
publishDate	2015
url	https://doaj.org/article/b95b39ad21a64c8f8c13abb6a0ee1bff
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Laboratory Activity to Teach about the Proliferation of <italic toggle="yes">Salmonella</italic> in Vegetables

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