De Novo Production of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide in Saccharomyces cerevisiae

Glycyrrhetic acid 3-O-mono-β-D-glucuronide (GAMG) is a rare compound in licorice and its short supply limits the wide applications in the pharmaceutical, cosmetic, and food industries. In this study, de novo biosynthesis of GAMG was achieved in engineered Saccharomyces cerevisiae strains based on th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ying Huang, Dan Jiang, Guangxi Ren, Yan Yin, Yifan Sun, Tengfei Liu, Chunsheng Liu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	glycyrrhetic acid 3-O-mono-β-D-glucuronide (GAMG) Saccharomyces cerevisiae cytochrome P450 enzymes (CYPs) CRISPR/Cas9 metabolic engineering Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/b968d1021b874823a62e52acb421dac0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b968d1021b874823a62e52acb421dac0
record_format	dspace
spelling	oai:doaj.org-article:b968d1021b874823a62e52acb421dac02021-11-30T13:32:53ZDe Novo Production of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide in Saccharomyces cerevisiae2296-418510.3389/fbioe.2021.709120https://doaj.org/article/b968d1021b874823a62e52acb421dac02021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.709120/fullhttps://doaj.org/toc/2296-4185Glycyrrhetic acid 3-O-mono-β-D-glucuronide (GAMG) is a rare compound in licorice and its short supply limits the wide applications in the pharmaceutical, cosmetic, and food industries. In this study, de novo biosynthesis of GAMG was achieved in engineered Saccharomyces cerevisiae strains based on the CRISPR/Cas9 genome editing technology. The introduction of GAMG biosynthetic pathway resulted in the construction of a GAMG-producing yeast strain for the first time. Through optimizing the biosynthetic pathway, improving the folding and catalysis microenvironment for cytochrome P450 enzymes (CYPs), enhancing the supply of UDP-glucuronic acid (UDP-GlcA), preventing product degradation, and optimizing the fermentation conditions, the production of GAMG was increased from 0.02 μg/L to 92.00 μg/L in shake flasks (4,200-fold), and the conversion rate of glycyrrhetic acid (GA) to GAMG was higher than 56%. The engineered yeast strains provide an alternative approach for the production of glycosylated triterpenoids.Ying HuangDan JiangGuangxi RenYan YinYifan SunTengfei LiuChunsheng LiuFrontiers Media S.A.articleglycyrrhetic acid 3-O-mono-β-D-glucuronide (GAMG)Saccharomyces cerevisiaecytochrome P450 enzymes (CYPs)CRISPR/Cas9metabolic engineeringBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glycyrrhetic acid 3-O-mono-β-D-glucuronide (GAMG) Saccharomyces cerevisiae cytochrome P450 enzymes (CYPs) CRISPR/Cas9 metabolic engineering Biotechnology TP248.13-248.65
spellingShingle	glycyrrhetic acid 3-O-mono-β-D-glucuronide (GAMG) Saccharomyces cerevisiae cytochrome P450 enzymes (CYPs) CRISPR/Cas9 metabolic engineering Biotechnology TP248.13-248.65 Ying Huang Dan Jiang Guangxi Ren Yan Yin Yifan Sun Tengfei Liu Chunsheng Liu De Novo Production of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide in Saccharomyces cerevisiae
description	Glycyrrhetic acid 3-O-mono-β-D-glucuronide (GAMG) is a rare compound in licorice and its short supply limits the wide applications in the pharmaceutical, cosmetic, and food industries. In this study, de novo biosynthesis of GAMG was achieved in engineered Saccharomyces cerevisiae strains based on the CRISPR/Cas9 genome editing technology. The introduction of GAMG biosynthetic pathway resulted in the construction of a GAMG-producing yeast strain for the first time. Through optimizing the biosynthetic pathway, improving the folding and catalysis microenvironment for cytochrome P450 enzymes (CYPs), enhancing the supply of UDP-glucuronic acid (UDP-GlcA), preventing product degradation, and optimizing the fermentation conditions, the production of GAMG was increased from 0.02 μg/L to 92.00 μg/L in shake flasks (4,200-fold), and the conversion rate of glycyrrhetic acid (GA) to GAMG was higher than 56%. The engineered yeast strains provide an alternative approach for the production of glycosylated triterpenoids.
format	article
author	Ying Huang Dan Jiang Guangxi Ren Yan Yin Yifan Sun Tengfei Liu Chunsheng Liu
author_facet	Ying Huang Dan Jiang Guangxi Ren Yan Yin Yifan Sun Tengfei Liu Chunsheng Liu
author_sort	Ying Huang
title	De Novo Production of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide in Saccharomyces cerevisiae
title_short	De Novo Production of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide in Saccharomyces cerevisiae
title_full	De Novo Production of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide in Saccharomyces cerevisiae
title_fullStr	De Novo Production of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide in Saccharomyces cerevisiae
title_full_unstemmed	De Novo Production of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide in Saccharomyces cerevisiae
title_sort	de novo production of glycyrrhetic acid 3-o-mono-β-d-glucuronide in saccharomyces cerevisiae
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b968d1021b874823a62e52acb421dac0
work_keys_str_mv	AT yinghuang denovoproductionofglycyrrheticacid3omonobdglucuronideinsaccharomycescerevisiae AT danjiang denovoproductionofglycyrrheticacid3omonobdglucuronideinsaccharomycescerevisiae AT guangxiren denovoproductionofglycyrrheticacid3omonobdglucuronideinsaccharomycescerevisiae AT yanyin denovoproductionofglycyrrheticacid3omonobdglucuronideinsaccharomycescerevisiae AT yifansun denovoproductionofglycyrrheticacid3omonobdglucuronideinsaccharomycescerevisiae AT tengfeiliu denovoproductionofglycyrrheticacid3omonobdglucuronideinsaccharomycescerevisiae AT chunshengliu denovoproductionofglycyrrheticacid3omonobdglucuronideinsaccharomycescerevisiae
_version_	1718406586087505920

De Novo Production of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide in Saccharomyces cerevisiae

Ejemplares similares