Gradual Histopathologic Changes During Development of Colorectal Cancer

Background: Colorectal cancer is one of the most common causes of mortality in the world.Objectives: The aim of this study was to investigate the histopathologic changes including hyperchromatism, tissue lymphocyteinfiltrations (TILs), aberrant crypt foci (ACF), microvessel density (MVD), p53, Bcl-2...

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Autores principales:	Vahid Nejati, Jamileh Abedi, Maedeh Vakili Saatloo, Maryam Koohsoltani, Rahim Hobbenaghi, Amir Tukmachi
Formato:	article
Lenguaje:	EN
Publicado:	Shiraz University of Medical Sciences 2019
Materias:	colorectal cancer tumor-infiltrating lymphocytes (tils) angiogenesis hyperchromatism microvessel density (mvd) aberrant crypt foci (acf) Medicine R
Acceso en línea:	https://doaj.org/article/b96f7837a4c24a02804fdeccecce353d
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spelling	oai:doaj.org-article:b96f7837a4c24a02804fdeccecce353d2021-11-14T08:42:11ZGradual Histopathologic Changes During Development of Colorectal Cancer2783-2430https://doaj.org/article/b96f7837a4c24a02804fdeccecce353d2019-03-01T00:00:00Zhttps://colorectalresearch.sums.ac.ir/article_47188_b8b0d2034d600892a545207c48126f51.pdfhttps://doaj.org/toc/2783-2430Background: Colorectal cancer is one of the most common causes of mortality in the world.Objectives: The aim of this study was to investigate the histopathologic changes including hyperchromatism, tissue lymphocyteinfiltrations (TILs), aberrant crypt foci (ACF), microvessel density (MVD), p53, Bcl-2 and CD31 changes during colorectal cancer development.Methods: Subcutaneous injections of dimethyl hydrazine DMH were administered to rats (40 mg/kg body weight) for 10 weeks.Rats were fed by food and water until 40th week and sacrificed two by two within 10, 15, 20, 25, 30 and 40 weeks after the start oftreatment. Thin paraffinized sections were applied to anti-CD31, anti-Bcl-2 and anti-p53 staining procedures. MVD and ACF werereported as mean value of three HPFs.Results: Hyperchromatism, TILs and angiogenesis were the most common initial histologic changes which started at 10th week ofDMH treatment. Hyperchromatism’s severity increased earlier than other changes and reached the highest value at the 25th week.The highest value of all variants occurred in the 40th week except the TILs which started to achieve the highest value in week 30 andincreased until 40th week. A diminished amount of p53 was observed at week 40, however, increased intensity of CD31 and Bcl-2were seen between 30th and 40th week.Conclusions: In conclusion, TILs and angiogenesis might be the important earliest factors contributing to colorectal cancer progression.Vahid NejatiJamileh AbediMaedeh Vakili SaatlooMaryam KoohsoltaniRahim HobbenaghiAmir TukmachiShiraz University of Medical Sciencesarticlecolorectal cancertumor-infiltrating lymphocytes (tils)angiogenesishyperchromatismmicrovessel density (mvd)aberrant crypt foci (acf)MedicineRENIranian Journal of Colorectal Research, Vol 7, Iss 1 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	colorectal cancer tumor-infiltrating lymphocytes (tils) angiogenesis hyperchromatism microvessel density (mvd) aberrant crypt foci (acf) Medicine R
spellingShingle	colorectal cancer tumor-infiltrating lymphocytes (tils) angiogenesis hyperchromatism microvessel density (mvd) aberrant crypt foci (acf) Medicine R Vahid Nejati Jamileh Abedi Maedeh Vakili Saatloo Maryam Koohsoltani Rahim Hobbenaghi Amir Tukmachi Gradual Histopathologic Changes During Development of Colorectal Cancer
description	Background: Colorectal cancer is one of the most common causes of mortality in the world.Objectives: The aim of this study was to investigate the histopathologic changes including hyperchromatism, tissue lymphocyteinfiltrations (TILs), aberrant crypt foci (ACF), microvessel density (MVD), p53, Bcl-2 and CD31 changes during colorectal cancer development.Methods: Subcutaneous injections of dimethyl hydrazine DMH were administered to rats (40 mg/kg body weight) for 10 weeks.Rats were fed by food and water until 40th week and sacrificed two by two within 10, 15, 20, 25, 30 and 40 weeks after the start oftreatment. Thin paraffinized sections were applied to anti-CD31, anti-Bcl-2 and anti-p53 staining procedures. MVD and ACF werereported as mean value of three HPFs.Results: Hyperchromatism, TILs and angiogenesis were the most common initial histologic changes which started at 10th week ofDMH treatment. Hyperchromatism’s severity increased earlier than other changes and reached the highest value at the 25th week.The highest value of all variants occurred in the 40th week except the TILs which started to achieve the highest value in week 30 andincreased until 40th week. A diminished amount of p53 was observed at week 40, however, increased intensity of CD31 and Bcl-2were seen between 30th and 40th week.Conclusions: In conclusion, TILs and angiogenesis might be the important earliest factors contributing to colorectal cancer progression.
format	article
author	Vahid Nejati Jamileh Abedi Maedeh Vakili Saatloo Maryam Koohsoltani Rahim Hobbenaghi Amir Tukmachi
author_facet	Vahid Nejati Jamileh Abedi Maedeh Vakili Saatloo Maryam Koohsoltani Rahim Hobbenaghi Amir Tukmachi
author_sort	Vahid Nejati
title	Gradual Histopathologic Changes During Development of Colorectal Cancer
title_short	Gradual Histopathologic Changes During Development of Colorectal Cancer
title_full	Gradual Histopathologic Changes During Development of Colorectal Cancer
title_fullStr	Gradual Histopathologic Changes During Development of Colorectal Cancer
title_full_unstemmed	Gradual Histopathologic Changes During Development of Colorectal Cancer
title_sort	gradual histopathologic changes during development of colorectal cancer
publisher	Shiraz University of Medical Sciences
publishDate	2019
url	https://doaj.org/article/b96f7837a4c24a02804fdeccecce353d
work_keys_str_mv	AT vahidnejati gradualhistopathologicchangesduringdevelopmentofcolorectalcancer AT jamilehabedi gradualhistopathologicchangesduringdevelopmentofcolorectalcancer AT maedehvakilisaatloo gradualhistopathologicchangesduringdevelopmentofcolorectalcancer AT maryamkoohsoltani gradualhistopathologicchangesduringdevelopmentofcolorectalcancer AT rahimhobbenaghi gradualhistopathologicchangesduringdevelopmentofcolorectalcancer AT amirtukmachi gradualhistopathologicchangesduringdevelopmentofcolorectalcancer
_version_	1718429800623767552

Gradual Histopathologic Changes During Development of Colorectal Cancer

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