Multi‐omics analysis reveals the interaction between the complement system and the coagulation cascade in the development of endometriosis

Abstract Endometriosis (EMS) is a disease that shows immune dysfunction and chronic inflammation characteristics, suggesting a role of complement system in its pathophysiology. To find out the hub genes and pathways involved in the pathogenesis of EMs, three raw microarray datasets were recruited fr...

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Autores principales:	Liang Yu, Huaji Shen, Xiaohan Ren, Anqi Wang, Shu Zhu, Yafeng Zheng, Xiuli Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b972ae1dac4b4772bf4ed2bbfa17278b
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spelling	oai:doaj.org-article:b972ae1dac4b4772bf4ed2bbfa17278b2021-12-02T17:30:34ZMulti‐omics analysis reveals the interaction between the complement system and the coagulation cascade in the development of endometriosis10.1038/s41598-021-90112-x2045-2322https://doaj.org/article/b972ae1dac4b4772bf4ed2bbfa17278b2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90112-xhttps://doaj.org/toc/2045-2322Abstract Endometriosis (EMS) is a disease that shows immune dysfunction and chronic inflammation characteristics, suggesting a role of complement system in its pathophysiology. To find out the hub genes and pathways involved in the pathogenesis of EMs, three raw microarray datasets were recruited from the Gene Expression Omnibus database (GEO). Then, a series of bioinformatics technologies including gene ontology (GO), Hallmark pathway enrichment, protein–protein interaction (PPI) network and gene co-expression correlation analysis were performed to identify hub genes. The hub genes were further verified by the Real-time quantitative polymerase chain reaction (RT-PCR) and Western Blot (WB). We identified 129 differentially expressed genes (DEGs) in EMs, of which 78 were up-regulated and 51 were down-regulated. Through GO functional enrichment analysis, we found that the DEGs are mainly enriched in cell adhesion, extracellular matrix remodeling, chemokine regulation, angiogenesis regulation, epithelial cell proliferation, et al. In Hallmark pathway enrichment analysis, coagulation pathway showed great significance and the terms in which included the central complement factors. Moreover, the genes were dominating in PPI network. Combined co-expression analysis with experimental verification, we found that the up-regulated expression of complement (C1S, C1QA, C1R, and C3) was positively related to tissue factor (TF) in EMs. In this study, we discovered the over expression complement and the positive correlation between complement and TF in EMs, which suggested that interaction of complement and coagulation system may play a role within the pathophysiology of EMS.Liang YuHuaji ShenXiaohan RenAnqi WangShu ZhuYafeng ZhengXiuli WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Liang Yu Huaji Shen Xiaohan Ren Anqi Wang Shu Zhu Yafeng Zheng Xiuli Wang Multi‐omics analysis reveals the interaction between the complement system and the coagulation cascade in the development of endometriosis
description	Abstract Endometriosis (EMS) is a disease that shows immune dysfunction and chronic inflammation characteristics, suggesting a role of complement system in its pathophysiology. To find out the hub genes and pathways involved in the pathogenesis of EMs, three raw microarray datasets were recruited from the Gene Expression Omnibus database (GEO). Then, a series of bioinformatics technologies including gene ontology (GO), Hallmark pathway enrichment, protein–protein interaction (PPI) network and gene co-expression correlation analysis were performed to identify hub genes. The hub genes were further verified by the Real-time quantitative polymerase chain reaction (RT-PCR) and Western Blot (WB). We identified 129 differentially expressed genes (DEGs) in EMs, of which 78 were up-regulated and 51 were down-regulated. Through GO functional enrichment analysis, we found that the DEGs are mainly enriched in cell adhesion, extracellular matrix remodeling, chemokine regulation, angiogenesis regulation, epithelial cell proliferation, et al. In Hallmark pathway enrichment analysis, coagulation pathway showed great significance and the terms in which included the central complement factors. Moreover, the genes were dominating in PPI network. Combined co-expression analysis with experimental verification, we found that the up-regulated expression of complement (C1S, C1QA, C1R, and C3) was positively related to tissue factor (TF) in EMs. In this study, we discovered the over expression complement and the positive correlation between complement and TF in EMs, which suggested that interaction of complement and coagulation system may play a role within the pathophysiology of EMS.
format	article
author	Liang Yu Huaji Shen Xiaohan Ren Anqi Wang Shu Zhu Yafeng Zheng Xiuli Wang
author_facet	Liang Yu Huaji Shen Xiaohan Ren Anqi Wang Shu Zhu Yafeng Zheng Xiuli Wang
author_sort	Liang Yu
title	Multi‐omics analysis reveals the interaction between the complement system and the coagulation cascade in the development of endometriosis
title_short	Multi‐omics analysis reveals the interaction between the complement system and the coagulation cascade in the development of endometriosis
title_full	Multi‐omics analysis reveals the interaction between the complement system and the coagulation cascade in the development of endometriosis
title_fullStr	Multi‐omics analysis reveals the interaction between the complement system and the coagulation cascade in the development of endometriosis
title_full_unstemmed	Multi‐omics analysis reveals the interaction between the complement system and the coagulation cascade in the development of endometriosis
title_sort	multi‐omics analysis reveals the interaction between the complement system and the coagulation cascade in the development of endometriosis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b972ae1dac4b4772bf4ed2bbfa17278b
work_keys_str_mv	AT liangyu multiomicsanalysisrevealstheinteractionbetweenthecomplementsystemandthecoagulationcascadeinthedevelopmentofendometriosis AT huajishen multiomicsanalysisrevealstheinteractionbetweenthecomplementsystemandthecoagulationcascadeinthedevelopmentofendometriosis AT xiaohanren multiomicsanalysisrevealstheinteractionbetweenthecomplementsystemandthecoagulationcascadeinthedevelopmentofendometriosis AT anqiwang multiomicsanalysisrevealstheinteractionbetweenthecomplementsystemandthecoagulationcascadeinthedevelopmentofendometriosis AT shuzhu multiomicsanalysisrevealstheinteractionbetweenthecomplementsystemandthecoagulationcascadeinthedevelopmentofendometriosis AT yafengzheng multiomicsanalysisrevealstheinteractionbetweenthecomplementsystemandthecoagulationcascadeinthedevelopmentofendometriosis AT xiuliwang multiomicsanalysisrevealstheinteractionbetweenthecomplementsystemandthecoagulationcascadeinthedevelopmentofendometriosis
_version_	1718380750685863936

Multi‐omics analysis reveals the interaction between the complement system and the coagulation cascade in the development of endometriosis

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