A Single-Cell Approach to the Elusive Latent Human Cytomegalovirus Transcriptome

ABSTRACT Herpesvirus latency has been difficult to understand molecularly due to low levels of viral genomes and gene expression. In the case of the betaherpesvirus human cytomegalovirus (HCMV), this is further complicated by the heterogeneity inherent to hematopoietic subpopulations harboring genom...

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Autores principales:	Felicia Goodrum, Shannon McWeeney
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	MARS-Seq cytomegalovirus herpesviruses scRNA-seq transcriptome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b9779c980a014f0887ecb0a542b2f9bc
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spelling	oai:doaj.org-article:b9779c980a014f0887ecb0a542b2f9bc2021-11-15T16:00:27ZA Single-Cell Approach to the Elusive Latent Human Cytomegalovirus Transcriptome10.1128/mBio.01001-182150-7511https://doaj.org/article/b9779c980a014f0887ecb0a542b2f9bc2018-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01001-18https://doaj.org/toc/2150-7511ABSTRACT Herpesvirus latency has been difficult to understand molecularly due to low levels of viral genomes and gene expression. In the case of the betaherpesvirus human cytomegalovirus (HCMV), this is further complicated by the heterogeneity inherent to hematopoietic subpopulations harboring genomes and, as a consequence, the various patterns of infection that simultaneously exist in a host, ranging from latent to lytic. Single-cell RNA sequencing (scRNA-seq) provides tremendous potential in measuring the gene expression profiles of heterogeneous cell populations for a wide range of applications, including in studies of cancer, immunology, and infectious disease. A recent study by Shnayder et al. (mBio 9:e00013-18, 2018, https://doi.org/10.1128/mBio.00013-18) utilized scRNA-seq to define transcriptomal characteristics of HCMV latency. They conclude that latency-associated gene expression is similar to the late lytic viral program but at lower levels of expression. The study highlights the numerous challenges, from the definition of latency to the analysis of scRNA-seq, that exist in defining a latent transcriptome.Felicia GoodrumShannon McWeeneyAmerican Society for MicrobiologyarticleMARS-SeqcytomegalovirusherpesvirusesscRNA-seqtranscriptomeMicrobiologyQR1-502ENmBio, Vol 9, Iss 3 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	MARS-Seq cytomegalovirus herpesviruses scRNA-seq transcriptome Microbiology QR1-502
spellingShingle	MARS-Seq cytomegalovirus herpesviruses scRNA-seq transcriptome Microbiology QR1-502 Felicia Goodrum Shannon McWeeney A Single-Cell Approach to the Elusive Latent Human Cytomegalovirus Transcriptome
description	ABSTRACT Herpesvirus latency has been difficult to understand molecularly due to low levels of viral genomes and gene expression. In the case of the betaherpesvirus human cytomegalovirus (HCMV), this is further complicated by the heterogeneity inherent to hematopoietic subpopulations harboring genomes and, as a consequence, the various patterns of infection that simultaneously exist in a host, ranging from latent to lytic. Single-cell RNA sequencing (scRNA-seq) provides tremendous potential in measuring the gene expression profiles of heterogeneous cell populations for a wide range of applications, including in studies of cancer, immunology, and infectious disease. A recent study by Shnayder et al. (mBio 9:e00013-18, 2018, https://doi.org/10.1128/mBio.00013-18) utilized scRNA-seq to define transcriptomal characteristics of HCMV latency. They conclude that latency-associated gene expression is similar to the late lytic viral program but at lower levels of expression. The study highlights the numerous challenges, from the definition of latency to the analysis of scRNA-seq, that exist in defining a latent transcriptome.
format	article
author	Felicia Goodrum Shannon McWeeney
author_facet	Felicia Goodrum Shannon McWeeney
author_sort	Felicia Goodrum
title	A Single-Cell Approach to the Elusive Latent Human Cytomegalovirus Transcriptome
title_short	A Single-Cell Approach to the Elusive Latent Human Cytomegalovirus Transcriptome
title_full	A Single-Cell Approach to the Elusive Latent Human Cytomegalovirus Transcriptome
title_fullStr	A Single-Cell Approach to the Elusive Latent Human Cytomegalovirus Transcriptome
title_full_unstemmed	A Single-Cell Approach to the Elusive Latent Human Cytomegalovirus Transcriptome
title_sort	single-cell approach to the elusive latent human cytomegalovirus transcriptome
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/b9779c980a014f0887ecb0a542b2f9bc
work_keys_str_mv	AT feliciagoodrum asinglecellapproachtotheelusivelatenthumancytomegalovirustranscriptome AT shannonmcweeney asinglecellapproachtotheelusivelatenthumancytomegalovirustranscriptome AT feliciagoodrum singlecellapproachtotheelusivelatenthumancytomegalovirustranscriptome AT shannonmcweeney singlecellapproachtotheelusivelatenthumancytomegalovirustranscriptome
_version_	1718426991087058944

A Single-Cell Approach to the Elusive Latent Human Cytomegalovirus Transcriptome

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