Super-Enhancers and Broad H3K4me3 Domains Form Complex Gene Regulatory Circuits Involving Chromatin Interactions

Abstract Stretched histone regions, such as super-enhancers and broad H3K4me3 domains, are associated with maintenance of cell identity and cancer. We connected super-enhancers and broad H3K4me3 domains in the K562 chronic myelogenous leukemia cell line as well as the MCF-7 breast cancer cell line w...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Fan Cao, Yiwen Fang, Hong Kee Tan, Yufen Goh, Jocelyn Yeen Hui Choy, Bryan Thean Howe Koh, Jiong Hao Tan, Nicolas Bertin, Aroul Ramadass, Ewan Hunter, Jayne Green, Matthew Salter, Alexandre Akoulitchev, Wilson Wang, Wee Joo Chng, Daniel G. Tenen, Melissa J. Fullwood
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b9841c5e788645b792bb546cfc9f0e3c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b9841c5e788645b792bb546cfc9f0e3c
record_format	dspace
spelling	oai:doaj.org-article:b9841c5e788645b792bb546cfc9f0e3c2021-12-02T12:30:45ZSuper-Enhancers and Broad H3K4me3 Domains Form Complex Gene Regulatory Circuits Involving Chromatin Interactions10.1038/s41598-017-02257-32045-2322https://doaj.org/article/b9841c5e788645b792bb546cfc9f0e3c2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02257-3https://doaj.org/toc/2045-2322Abstract Stretched histone regions, such as super-enhancers and broad H3K4me3 domains, are associated with maintenance of cell identity and cancer. We connected super-enhancers and broad H3K4me3 domains in the K562 chronic myelogenous leukemia cell line as well as the MCF-7 breast cancer cell line with chromatin interactions. Super-enhancers and broad H3K4me3 domains showed higher association with chromatin interactions than their typical counterparts. Interestingly, we identified a subset of super-enhancers that overlap with broad H3K4me3 domains and show high association with cancer-associated genes including tumor suppressor genes. Besides cell lines, we could observe chromatin interactions by a Chromosome Conformation Capture (3C)-based method, in primary human samples. Several chromatin interactions involving super-enhancers and broad H3K4me3 domains are constitutive and can be found in both cancer and normal samples. Taken together, these results reveal a new layer of complexity in gene regulation by super-enhancers and broad H3K4me3 domains.Fan CaoYiwen FangHong Kee TanYufen GohJocelyn Yeen Hui ChoyBryan Thean Howe KohJiong Hao TanNicolas BertinAroul RamadassEwan HunterJayne GreenMatthew SalterAlexandre AkoulitchevWilson WangWee Joo ChngDaniel G. TenenMelissa J. FullwoodNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fan Cao Yiwen Fang Hong Kee Tan Yufen Goh Jocelyn Yeen Hui Choy Bryan Thean Howe Koh Jiong Hao Tan Nicolas Bertin Aroul Ramadass Ewan Hunter Jayne Green Matthew Salter Alexandre Akoulitchev Wilson Wang Wee Joo Chng Daniel G. Tenen Melissa J. Fullwood Super-Enhancers and Broad H3K4me3 Domains Form Complex Gene Regulatory Circuits Involving Chromatin Interactions
description	Abstract Stretched histone regions, such as super-enhancers and broad H3K4me3 domains, are associated with maintenance of cell identity and cancer. We connected super-enhancers and broad H3K4me3 domains in the K562 chronic myelogenous leukemia cell line as well as the MCF-7 breast cancer cell line with chromatin interactions. Super-enhancers and broad H3K4me3 domains showed higher association with chromatin interactions than their typical counterparts. Interestingly, we identified a subset of super-enhancers that overlap with broad H3K4me3 domains and show high association with cancer-associated genes including tumor suppressor genes. Besides cell lines, we could observe chromatin interactions by a Chromosome Conformation Capture (3C)-based method, in primary human samples. Several chromatin interactions involving super-enhancers and broad H3K4me3 domains are constitutive and can be found in both cancer and normal samples. Taken together, these results reveal a new layer of complexity in gene regulation by super-enhancers and broad H3K4me3 domains.
format	article
author	Fan Cao Yiwen Fang Hong Kee Tan Yufen Goh Jocelyn Yeen Hui Choy Bryan Thean Howe Koh Jiong Hao Tan Nicolas Bertin Aroul Ramadass Ewan Hunter Jayne Green Matthew Salter Alexandre Akoulitchev Wilson Wang Wee Joo Chng Daniel G. Tenen Melissa J. Fullwood
author_facet	Fan Cao Yiwen Fang Hong Kee Tan Yufen Goh Jocelyn Yeen Hui Choy Bryan Thean Howe Koh Jiong Hao Tan Nicolas Bertin Aroul Ramadass Ewan Hunter Jayne Green Matthew Salter Alexandre Akoulitchev Wilson Wang Wee Joo Chng Daniel G. Tenen Melissa J. Fullwood
author_sort	Fan Cao
title	Super-Enhancers and Broad H3K4me3 Domains Form Complex Gene Regulatory Circuits Involving Chromatin Interactions
title_short	Super-Enhancers and Broad H3K4me3 Domains Form Complex Gene Regulatory Circuits Involving Chromatin Interactions
title_full	Super-Enhancers and Broad H3K4me3 Domains Form Complex Gene Regulatory Circuits Involving Chromatin Interactions
title_fullStr	Super-Enhancers and Broad H3K4me3 Domains Form Complex Gene Regulatory Circuits Involving Chromatin Interactions
title_full_unstemmed	Super-Enhancers and Broad H3K4me3 Domains Form Complex Gene Regulatory Circuits Involving Chromatin Interactions
title_sort	super-enhancers and broad h3k4me3 domains form complex gene regulatory circuits involving chromatin interactions
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b9841c5e788645b792bb546cfc9f0e3c
work_keys_str_mv	AT fancao superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions AT yiwenfang superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions AT hongkeetan superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions AT yufengoh superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions AT jocelynyeenhuichoy superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions AT bryantheanhowekoh superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions AT jionghaotan superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions AT nicolasbertin superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions AT aroulramadass superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions AT ewanhunter superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions AT jaynegreen superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions AT matthewsalter superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions AT alexandreakoulitchev superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions AT wilsonwang superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions AT weejoochng superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions AT danielgtenen superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions AT melissajfullwood superenhancersandbroadh3k4me3domainsformcomplexgeneregulatorycircuitsinvolvingchromatininteractions
_version_	1718394357358264320

Super-Enhancers and Broad H3K4me3 Domains Form Complex Gene Regulatory Circuits Involving Chromatin Interactions

Ejemplares similares