Protective effect of pre- and post-vitamin C treatments on UVB-irradiation-induced skin damage

Abstract Several studies have reported the effects of vitamin C (L-ascorbic acid, AA) on ultraviolet B (UVB)-induced cell damage using cultured keratinocytes. However, the epidermis consists of multiple cell layers, and the effect of AA on UVB-induced damage to the human epidermis remains unclear. T...

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Autores principales:	Saki Kawashima, Tomoko Funakoshi, Yasunori Sato, Norikatsu Saito, Hajime Ohsawa, Katsumi Kurita, Kisaburo Nagata, Masayuki Yoshida, Akihito Ishigami
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Skin Damage Epidermal Surface Multiple Cell Layers Transferase-mediated dUTP-biotin Nick End Labeling (TUNEL) Senescence Marker Protein-30 (SMP30) Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b98c4a4336064ef5ae87cdfa876e5c6c
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spelling	oai:doaj.org-article:b98c4a4336064ef5ae87cdfa876e5c6c2021-12-02T15:09:06ZProtective effect of pre- and post-vitamin C treatments on UVB-irradiation-induced skin damage10.1038/s41598-018-34530-42045-2322https://doaj.org/article/b98c4a4336064ef5ae87cdfa876e5c6c2018-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34530-4https://doaj.org/toc/2045-2322Abstract Several studies have reported the effects of vitamin C (L-ascorbic acid, AA) on ultraviolet B (UVB)-induced cell damage using cultured keratinocytes. However, the epidermis consists of multiple cell layers, and the effect of AA on UVB-induced damage to the human epidermis remains unclear. Therefore, we investigated the effect of AA on UVB-induced skin damage using reconstituted human epidermis. The reconstituted human epidermal surface was treated with 100 and 500 mM AA and cultured for 3 h before (pre-AA treatment) or after (post-AA treatment) 120 mJ/cm2 UVB irradiation. Pre- and post-AA treatments of the epidermal surface suppressed UVB-induced cell death, apoptosis, DNA damage, reactive oxygen species (ROS) production, and the inflammatory response by downregulating tumour necrosis factor-α (TNF-α) expression and release. Moreover, the pre-AA treatment was more effective at preventing UVB-induced skin damage than the post-AA treatment. In summary, pre- and post-AA treatments of the epidermis prevent UVB-induced damage.Saki KawashimaTomoko FunakoshiYasunori SatoNorikatsu SaitoHajime OhsawaKatsumi KuritaKisaburo NagataMasayuki YoshidaAkihito IshigamiNature PortfolioarticleSkin DamageEpidermal SurfaceMultiple Cell LayersTransferase-mediated dUTP-biotin Nick End Labeling (TUNEL)Senescence Marker Protein-30 (SMP30)MedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Skin Damage Epidermal Surface Multiple Cell Layers Transferase-mediated dUTP-biotin Nick End Labeling (TUNEL) Senescence Marker Protein-30 (SMP30) Medicine R Science Q
spellingShingle	Skin Damage Epidermal Surface Multiple Cell Layers Transferase-mediated dUTP-biotin Nick End Labeling (TUNEL) Senescence Marker Protein-30 (SMP30) Medicine R Science Q Saki Kawashima Tomoko Funakoshi Yasunori Sato Norikatsu Saito Hajime Ohsawa Katsumi Kurita Kisaburo Nagata Masayuki Yoshida Akihito Ishigami Protective effect of pre- and post-vitamin C treatments on UVB-irradiation-induced skin damage
description	Abstract Several studies have reported the effects of vitamin C (L-ascorbic acid, AA) on ultraviolet B (UVB)-induced cell damage using cultured keratinocytes. However, the epidermis consists of multiple cell layers, and the effect of AA on UVB-induced damage to the human epidermis remains unclear. Therefore, we investigated the effect of AA on UVB-induced skin damage using reconstituted human epidermis. The reconstituted human epidermal surface was treated with 100 and 500 mM AA and cultured for 3 h before (pre-AA treatment) or after (post-AA treatment) 120 mJ/cm2 UVB irradiation. Pre- and post-AA treatments of the epidermal surface suppressed UVB-induced cell death, apoptosis, DNA damage, reactive oxygen species (ROS) production, and the inflammatory response by downregulating tumour necrosis factor-α (TNF-α) expression and release. Moreover, the pre-AA treatment was more effective at preventing UVB-induced skin damage than the post-AA treatment. In summary, pre- and post-AA treatments of the epidermis prevent UVB-induced damage.
format	article
author	Saki Kawashima Tomoko Funakoshi Yasunori Sato Norikatsu Saito Hajime Ohsawa Katsumi Kurita Kisaburo Nagata Masayuki Yoshida Akihito Ishigami
author_facet	Saki Kawashima Tomoko Funakoshi Yasunori Sato Norikatsu Saito Hajime Ohsawa Katsumi Kurita Kisaburo Nagata Masayuki Yoshida Akihito Ishigami
author_sort	Saki Kawashima
title	Protective effect of pre- and post-vitamin C treatments on UVB-irradiation-induced skin damage
title_short	Protective effect of pre- and post-vitamin C treatments on UVB-irradiation-induced skin damage
title_full	Protective effect of pre- and post-vitamin C treatments on UVB-irradiation-induced skin damage
title_fullStr	Protective effect of pre- and post-vitamin C treatments on UVB-irradiation-induced skin damage
title_full_unstemmed	Protective effect of pre- and post-vitamin C treatments on UVB-irradiation-induced skin damage
title_sort	protective effect of pre- and post-vitamin c treatments on uvb-irradiation-induced skin damage
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b98c4a4336064ef5ae87cdfa876e5c6c
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Protective effect of pre- and post-vitamin C treatments on UVB-irradiation-induced skin damage

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