Collection and chemical composition of phloem sap from Citrus sinensis L. Osbeck (sweet orange).

Through utilizing the nutrient-rich phloem sap, sap feeding insects such as psyllids, leafhoppers, and aphids can transmit many phloem-restricted pathogens. On the other hand, multiplication of phloem-limited, uncultivated bacteria such as Candidatus Liberibacter asiaticus (CLas) inside the phloem o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Faraj Hijaz, Nabil Killiny
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b98d157fe2e340e7908f0a39403dcd73
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b98d157fe2e340e7908f0a39403dcd73
record_format	dspace
spelling	oai:doaj.org-article:b98d157fe2e340e7908f0a39403dcd732021-11-25T06:08:48ZCollection and chemical composition of phloem sap from Citrus sinensis L. Osbeck (sweet orange).1932-620310.1371/journal.pone.0101830https://doaj.org/article/b98d157fe2e340e7908f0a39403dcd732014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25014027/?tool=EBIhttps://doaj.org/toc/1932-6203Through utilizing the nutrient-rich phloem sap, sap feeding insects such as psyllids, leafhoppers, and aphids can transmit many phloem-restricted pathogens. On the other hand, multiplication of phloem-limited, uncultivated bacteria such as Candidatus Liberibacter asiaticus (CLas) inside the phloem of citrus indicates that the sap contains all the essential nutrients needed for the pathogen growth. The phloem sap composition of many plants has been studied; however, to our knowledge, there is no available data about citrus phloem sap. In this study, we identified and quantified the chemical components of phloem sap from pineapple sweet orange. Two approaches (EDTA enhanced exudation and centrifugation) were used to collect phloem sap. The collected sap was derivatized with methyl chloroformate (MCF), N-methyl-N- [tert-butyl dimethylsilyl]-trifluroacetamide (MTBSTFA), or trimethylsilyl (TMS) and analyzed with GC-MS revealing 20 amino acids and 8 sugars. Proline, the most abundant amino acid, composed more than 60% of the total amino acids. Tryptophan, tyrosine, leucine, isoleucine, and valine, which are considered essential for phloem sap-sucking insects, were also detected. Sucrose, glucose, fructose, and inositol were the most predominant sugars. In addition, seven organic acids including succinic, fumaric, malic, maleic, threonic, citric, and quinic were detected. All compounds detected in the EDTA-enhanced exudate were also detected in the pure phloem sap using centrifugation. The centrifugation technique allowed estimating the concentration of metabolites. This information expands our knowledge about the nutrition requirement for citrus phloem-limited bacterial pathogen and their vectors, and can help define suitable artificial media to culture them.Faraj HijazNabil KillinyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e101830 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Faraj Hijaz Nabil Killiny Collection and chemical composition of phloem sap from Citrus sinensis L. Osbeck (sweet orange).
description	Through utilizing the nutrient-rich phloem sap, sap feeding insects such as psyllids, leafhoppers, and aphids can transmit many phloem-restricted pathogens. On the other hand, multiplication of phloem-limited, uncultivated bacteria such as Candidatus Liberibacter asiaticus (CLas) inside the phloem of citrus indicates that the sap contains all the essential nutrients needed for the pathogen growth. The phloem sap composition of many plants has been studied; however, to our knowledge, there is no available data about citrus phloem sap. In this study, we identified and quantified the chemical components of phloem sap from pineapple sweet orange. Two approaches (EDTA enhanced exudation and centrifugation) were used to collect phloem sap. The collected sap was derivatized with methyl chloroformate (MCF), N-methyl-N- [tert-butyl dimethylsilyl]-trifluroacetamide (MTBSTFA), or trimethylsilyl (TMS) and analyzed with GC-MS revealing 20 amino acids and 8 sugars. Proline, the most abundant amino acid, composed more than 60% of the total amino acids. Tryptophan, tyrosine, leucine, isoleucine, and valine, which are considered essential for phloem sap-sucking insects, were also detected. Sucrose, glucose, fructose, and inositol were the most predominant sugars. In addition, seven organic acids including succinic, fumaric, malic, maleic, threonic, citric, and quinic were detected. All compounds detected in the EDTA-enhanced exudate were also detected in the pure phloem sap using centrifugation. The centrifugation technique allowed estimating the concentration of metabolites. This information expands our knowledge about the nutrition requirement for citrus phloem-limited bacterial pathogen and their vectors, and can help define suitable artificial media to culture them.
format	article
author	Faraj Hijaz Nabil Killiny
author_facet	Faraj Hijaz Nabil Killiny
author_sort	Faraj Hijaz
title	Collection and chemical composition of phloem sap from Citrus sinensis L. Osbeck (sweet orange).
title_short	Collection and chemical composition of phloem sap from Citrus sinensis L. Osbeck (sweet orange).
title_full	Collection and chemical composition of phloem sap from Citrus sinensis L. Osbeck (sweet orange).
title_fullStr	Collection and chemical composition of phloem sap from Citrus sinensis L. Osbeck (sweet orange).
title_full_unstemmed	Collection and chemical composition of phloem sap from Citrus sinensis L. Osbeck (sweet orange).
title_sort	collection and chemical composition of phloem sap from citrus sinensis l. osbeck (sweet orange).
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b98d157fe2e340e7908f0a39403dcd73
work_keys_str_mv	AT farajhijaz collectionandchemicalcompositionofphloemsapfromcitrussinensislosbecksweetorange AT nabilkilliny collectionandchemicalcompositionofphloemsapfromcitrussinensislosbecksweetorange
_version_	1718414126740406272

Collection and chemical composition of phloem sap from Citrus sinensis L. Osbeck (sweet orange).

Ejemplares similares