Ex vivo cytokine mRNA levels correlate with changing clinical status of ethiopian TB patients and their contacts over time.
There is an increasing body of evidence which suggests that IL-4 plays a role in the pathogenesis of TB, but a general consensus on its role remains elusive. We have previously published data from a cohort of Ethiopian TB patients, their contacts, and community controls suggesting that enhanced IL-4...
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2008
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oai:doaj.org-article:b98dc77a81ae4171ae70b5d7f4ba60592021-11-25T06:13:30ZEx vivo cytokine mRNA levels correlate with changing clinical status of ethiopian TB patients and their contacts over time.1932-620310.1371/journal.pone.0001522https://doaj.org/article/b98dc77a81ae4171ae70b5d7f4ba60592008-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18231607/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203There is an increasing body of evidence which suggests that IL-4 plays a role in the pathogenesis of TB, but a general consensus on its role remains elusive. We have previously published data from a cohort of Ethiopian TB patients, their contacts, and community controls suggesting that enhanced IL-4 production is associated with infection with M. tuberculosis, rather than overt disease and that long-term protection in infected community controls is associated with co-production of the IL-4 antagonist IL-4d2, alongside elevated IL-4. Here, for the first time, we compare data on expression of IFN-gamma, IL-4 and IL-4delta2 over time in TB patients and their household contacts. During the follow-up period, the TB patients completed therapy and ceased to display TB-like symptoms. This correlated with a decrease in the relative amount of IL-4 expressed. Over the same period, the clinical status of some of their contacts also changed, with a number developing TB-like symptoms or clinically apparent TB. IL-4 expression was disproportionately increased in this group. The findings support the hypothesis that elevated IL-4 production is generally associated with infection, but that TB disease is associated with a relatively increased expression of IL-4 compared to IFN-gamma and IL-4delta2. However, the data also suggest that there are no clear-cut differences between groups: the immune response over time appears to include changes in the expression of IFN-gamma, IL-4 and IL-4delta2, and it is the relative, not absolute levels of cytokine expression that are characteristic of clinical status.Liya WassieAbebech DemissieAbraham AseffaMarkos AbebeLawrence YamuahHiwot TilahunBeyene PetrosGraham RookAlimuddin ZumlaPeter AndersenT Mark DohertyVACSEL Study GroupPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 1, p e1522 (2008) |
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EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Liya Wassie Abebech Demissie Abraham Aseffa Markos Abebe Lawrence Yamuah Hiwot Tilahun Beyene Petros Graham Rook Alimuddin Zumla Peter Andersen T Mark Doherty VACSEL Study Group Ex vivo cytokine mRNA levels correlate with changing clinical status of ethiopian TB patients and their contacts over time. |
| description |
There is an increasing body of evidence which suggests that IL-4 plays a role in the pathogenesis of TB, but a general consensus on its role remains elusive. We have previously published data from a cohort of Ethiopian TB patients, their contacts, and community controls suggesting that enhanced IL-4 production is associated with infection with M. tuberculosis, rather than overt disease and that long-term protection in infected community controls is associated with co-production of the IL-4 antagonist IL-4d2, alongside elevated IL-4. Here, for the first time, we compare data on expression of IFN-gamma, IL-4 and IL-4delta2 over time in TB patients and their household contacts. During the follow-up period, the TB patients completed therapy and ceased to display TB-like symptoms. This correlated with a decrease in the relative amount of IL-4 expressed. Over the same period, the clinical status of some of their contacts also changed, with a number developing TB-like symptoms or clinically apparent TB. IL-4 expression was disproportionately increased in this group. The findings support the hypothesis that elevated IL-4 production is generally associated with infection, but that TB disease is associated with a relatively increased expression of IL-4 compared to IFN-gamma and IL-4delta2. However, the data also suggest that there are no clear-cut differences between groups: the immune response over time appears to include changes in the expression of IFN-gamma, IL-4 and IL-4delta2, and it is the relative, not absolute levels of cytokine expression that are characteristic of clinical status. |
| format |
article |
| author |
Liya Wassie Abebech Demissie Abraham Aseffa Markos Abebe Lawrence Yamuah Hiwot Tilahun Beyene Petros Graham Rook Alimuddin Zumla Peter Andersen T Mark Doherty VACSEL Study Group |
| author_facet |
Liya Wassie Abebech Demissie Abraham Aseffa Markos Abebe Lawrence Yamuah Hiwot Tilahun Beyene Petros Graham Rook Alimuddin Zumla Peter Andersen T Mark Doherty VACSEL Study Group |
| author_sort |
Liya Wassie |
| title |
Ex vivo cytokine mRNA levels correlate with changing clinical status of ethiopian TB patients and their contacts over time. |
| title_short |
Ex vivo cytokine mRNA levels correlate with changing clinical status of ethiopian TB patients and their contacts over time. |
| title_full |
Ex vivo cytokine mRNA levels correlate with changing clinical status of ethiopian TB patients and their contacts over time. |
| title_fullStr |
Ex vivo cytokine mRNA levels correlate with changing clinical status of ethiopian TB patients and their contacts over time. |
| title_full_unstemmed |
Ex vivo cytokine mRNA levels correlate with changing clinical status of ethiopian TB patients and their contacts over time. |
| title_sort |
ex vivo cytokine mrna levels correlate with changing clinical status of ethiopian tb patients and their contacts over time. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2008 |
| url |
https://doaj.org/article/b98dc77a81ae4171ae70b5d7f4ba6059 |
| work_keys_str_mv |
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