Histological and Immunohistochemical Studies on The Role of Stem Cells on The Burned Skin of Adult Male Albino Rats

Background: Mesenchymal stem cells have delivered new approaches to the management of burn healing in severe skin injuries. Aim of the work: To evaluate the effect of bone marrow-derived mesenchymal stem cells (BMSCs) on healing of induced deep 2nd degree skin burns in albino rats. Methodology: 55 m...

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Autores principales:	Fatma Abd Allah, Ashraf Moustafa, Lotfy Moahmmed, Ezz El-Dein Abd Allah
Formato:	article
Lenguaje:	EN
Publicado:	Al-Azhar University, Faculty of Medicine (Damietta) 2020
Materias:	stem cells skin vascular endothelial growth factor wound healing scar Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b99957c0dbf941068dd100cbb2e041a3
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spelling	oai:doaj.org-article:b99957c0dbf941068dd100cbb2e041a32021-12-02T13:52:45ZHistological and Immunohistochemical Studies on The Role of Stem Cells on The Burned Skin of Adult Male Albino Rats2636-41742682-378010.21608/ijma.2020.20386.1050https://doaj.org/article/b99957c0dbf941068dd100cbb2e041a32020-01-01T00:00:00Zhttps://ijma.journals.ekb.eg/article_69613_3bfd52629109c32cdb360df683a40519.pdfhttps://doaj.org/toc/2636-4174https://doaj.org/toc/2682-3780Background: Mesenchymal stem cells have delivered new approaches to the management of burn healing in severe skin injuries. Aim of the work: To evaluate the effect of bone marrow-derived mesenchymal stem cells (BMSCs) on healing of induced deep 2nd degree skin burns in albino rats. Methodology: 55 male albino rats weighed 200gm were divided into 5 groups, 5 animals in group I (control) and group II (burn model), 15 animals in group III (untreated), group IV and group V (MSCs treated). Groups III, IV and V were subdivided into 3 subgroups that were sacrificed after 7, 14 and 21 days respectively. Deep 2nd degree burn wounds were induced on a 4 cm2 area on the back of rats in groups II, III, IV and V by brass probes stabilized at 88–90°C and contacted to the shaved dorsal skin of rats for 20 seconds without exerting any external pressure. Mesenchymal stem cells were isolated from the bone marrow of 5 young rats, weighed 100gm then intra-dermally and intraperitoneally injected. Results: By the end of the third week, the wounds of BMSCs-treated groups showed full regeneration of epidermis, re-organization of collagen and decrease in VEGF immunopositive cells. Delayed wound healing was seen in 20% of systemically treated rats. Significant increase in the mean area percent of collagen fibers was detected in topically treated group. Conclusion: Both methods of BMSCs injection were effective in healing of full thickness skin wound but topical method was more effective.Fatma Abd AllahAshraf MoustafaLotfy MoahmmedEzz El-Dein Abd AllahAl-Azhar University, Faculty of Medicine (Damietta)articlestem cellsskinvascular endothelial growth factorwound healingscarMedicine (General)R5-920ENInternational Journal of Medical Arts, Vol 2, Iss 1, Pp 265-291 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	stem cells skin vascular endothelial growth factor wound healing scar Medicine (General) R5-920
spellingShingle	stem cells skin vascular endothelial growth factor wound healing scar Medicine (General) R5-920 Fatma Abd Allah Ashraf Moustafa Lotfy Moahmmed Ezz El-Dein Abd Allah Histological and Immunohistochemical Studies on The Role of Stem Cells on The Burned Skin of Adult Male Albino Rats
description	Background: Mesenchymal stem cells have delivered new approaches to the management of burn healing in severe skin injuries. Aim of the work: To evaluate the effect of bone marrow-derived mesenchymal stem cells (BMSCs) on healing of induced deep 2nd degree skin burns in albino rats. Methodology: 55 male albino rats weighed 200gm were divided into 5 groups, 5 animals in group I (control) and group II (burn model), 15 animals in group III (untreated), group IV and group V (MSCs treated). Groups III, IV and V were subdivided into 3 subgroups that were sacrificed after 7, 14 and 21 days respectively. Deep 2nd degree burn wounds were induced on a 4 cm2 area on the back of rats in groups II, III, IV and V by brass probes stabilized at 88–90°C and contacted to the shaved dorsal skin of rats for 20 seconds without exerting any external pressure. Mesenchymal stem cells were isolated from the bone marrow of 5 young rats, weighed 100gm then intra-dermally and intraperitoneally injected. Results: By the end of the third week, the wounds of BMSCs-treated groups showed full regeneration of epidermis, re-organization of collagen and decrease in VEGF immunopositive cells. Delayed wound healing was seen in 20% of systemically treated rats. Significant increase in the mean area percent of collagen fibers was detected in topically treated group. Conclusion: Both methods of BMSCs injection were effective in healing of full thickness skin wound but topical method was more effective.
format	article
author	Fatma Abd Allah Ashraf Moustafa Lotfy Moahmmed Ezz El-Dein Abd Allah
author_facet	Fatma Abd Allah Ashraf Moustafa Lotfy Moahmmed Ezz El-Dein Abd Allah
author_sort	Fatma Abd Allah
title	Histological and Immunohistochemical Studies on The Role of Stem Cells on The Burned Skin of Adult Male Albino Rats
title_short	Histological and Immunohistochemical Studies on The Role of Stem Cells on The Burned Skin of Adult Male Albino Rats
title_full	Histological and Immunohistochemical Studies on The Role of Stem Cells on The Burned Skin of Adult Male Albino Rats
title_fullStr	Histological and Immunohistochemical Studies on The Role of Stem Cells on The Burned Skin of Adult Male Albino Rats
title_full_unstemmed	Histological and Immunohistochemical Studies on The Role of Stem Cells on The Burned Skin of Adult Male Albino Rats
title_sort	histological and immunohistochemical studies on the role of stem cells on the burned skin of adult male albino rats
publisher	Al-Azhar University, Faculty of Medicine (Damietta)
publishDate	2020
url	https://doaj.org/article/b99957c0dbf941068dd100cbb2e041a3
work_keys_str_mv	AT fatmaabdallah histologicalandimmunohistochemicalstudiesontheroleofstemcellsontheburnedskinofadultmalealbinorats AT ashrafmoustafa histologicalandimmunohistochemicalstudiesontheroleofstemcellsontheburnedskinofadultmalealbinorats AT lotfymoahmmed histologicalandimmunohistochemicalstudiesontheroleofstemcellsontheburnedskinofadultmalealbinorats AT ezzeldeinabdallah histologicalandimmunohistochemicalstudiesontheroleofstemcellsontheburnedskinofadultmalealbinorats
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Histological and Immunohistochemical Studies on The Role of Stem Cells on The Burned Skin of Adult Male Albino Rats

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