Non-invasive in vivo fluorescence imaging of apoptotic retinal photoreceptors

Abstract Phosphatidylserine externalization is an early molecular signature for apoptosis. In many retinal degenerative diseases, photoreceptor neurons die by apoptosis. Here, we report utility of the phosphatidylserine-binding conjugate of Bis(zinc(II)-dipicolylamine (Zn-DPA) with Texas-red (PSVue-...

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Autores principales:	Francesca Mazzoni, Claudia Müller, Jonathan DeAssis, Deborah Lew, W. Matthew Leevy, Silvia C. Finnemann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b9a3c646aa1d4bb2a8a8cbe6cc25c001
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spelling	oai:doaj.org-article:b9a3c646aa1d4bb2a8a8cbe6cc25c0012021-12-02T16:08:43ZNon-invasive in vivo fluorescence imaging of apoptotic retinal photoreceptors10.1038/s41598-018-38363-z2045-2322https://doaj.org/article/b9a3c646aa1d4bb2a8a8cbe6cc25c0012019-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-38363-zhttps://doaj.org/toc/2045-2322Abstract Phosphatidylserine externalization is an early molecular signature for apoptosis. In many retinal degenerative diseases, photoreceptor neurons die by apoptosis. Here, we report utility of the phosphatidylserine-binding conjugate of Bis(zinc(II)-dipicolylamine (Zn-DPA) with Texas-red (PSVue-550) in transiently labeling apoptotic photoreceptors in living pigmented or albino rats and mice with retinal degeneration. Applying PSVue-550 as eyedrop is non-toxic and eliminates need for intraocular injection. PSVue-550 fluorescence specifically and transiently labeling dying retinal photoreceptors is detectable in anesthetized animals using standard retinal or whole small animal imaging systems. Importantly, prior PSVue-550 eyedrop administration and imaging does not affect repeat testing. Altogether, our results establish PSVue-550 imaging as a completely non-invasive method that provides the opportunity to longitudinally monitor retinal photoreceptor cell death in preclinical studies.Francesca MazzoniClaudia MüllerJonathan DeAssisDeborah LewW. Matthew LeevySilvia C. FinnemannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francesca Mazzoni Claudia Müller Jonathan DeAssis Deborah Lew W. Matthew Leevy Silvia C. Finnemann Non-invasive in vivo fluorescence imaging of apoptotic retinal photoreceptors
description	Abstract Phosphatidylserine externalization is an early molecular signature for apoptosis. In many retinal degenerative diseases, photoreceptor neurons die by apoptosis. Here, we report utility of the phosphatidylserine-binding conjugate of Bis(zinc(II)-dipicolylamine (Zn-DPA) with Texas-red (PSVue-550) in transiently labeling apoptotic photoreceptors in living pigmented or albino rats and mice with retinal degeneration. Applying PSVue-550 as eyedrop is non-toxic and eliminates need for intraocular injection. PSVue-550 fluorescence specifically and transiently labeling dying retinal photoreceptors is detectable in anesthetized animals using standard retinal or whole small animal imaging systems. Importantly, prior PSVue-550 eyedrop administration and imaging does not affect repeat testing. Altogether, our results establish PSVue-550 imaging as a completely non-invasive method that provides the opportunity to longitudinally monitor retinal photoreceptor cell death in preclinical studies.
format	article
author	Francesca Mazzoni Claudia Müller Jonathan DeAssis Deborah Lew W. Matthew Leevy Silvia C. Finnemann
author_facet	Francesca Mazzoni Claudia Müller Jonathan DeAssis Deborah Lew W. Matthew Leevy Silvia C. Finnemann
author_sort	Francesca Mazzoni
title	Non-invasive in vivo fluorescence imaging of apoptotic retinal photoreceptors
title_short	Non-invasive in vivo fluorescence imaging of apoptotic retinal photoreceptors
title_full	Non-invasive in vivo fluorescence imaging of apoptotic retinal photoreceptors
title_fullStr	Non-invasive in vivo fluorescence imaging of apoptotic retinal photoreceptors
title_full_unstemmed	Non-invasive in vivo fluorescence imaging of apoptotic retinal photoreceptors
title_sort	non-invasive in vivo fluorescence imaging of apoptotic retinal photoreceptors
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/b9a3c646aa1d4bb2a8a8cbe6cc25c001
work_keys_str_mv	AT francescamazzoni noninvasiveinvivofluorescenceimagingofapoptoticretinalphotoreceptors AT claudiamuller noninvasiveinvivofluorescenceimagingofapoptoticretinalphotoreceptors AT jonathandeassis noninvasiveinvivofluorescenceimagingofapoptoticretinalphotoreceptors AT deborahlew noninvasiveinvivofluorescenceimagingofapoptoticretinalphotoreceptors AT wmatthewleevy noninvasiveinvivofluorescenceimagingofapoptoticretinalphotoreceptors AT silviacfinnemann noninvasiveinvivofluorescenceimagingofapoptoticretinalphotoreceptors
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Non-invasive in vivo fluorescence imaging of apoptotic retinal photoreceptors

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