Expression of Matrix Metalloproteinase-9 in Different Histopathological Variants of Ameloblastoma: A Cross-sectional Study
Introduction: Ameloblastomas are benign and the most common odontogenic neoplasms with many histopathological subtypes depending on the predominant pattern. They are known for their aggressive behaviour. As ameloblastomas have a high rate of recurrence, it is necessary to understand the biologic...
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oai:doaj.org-article:b9a5af65335b440491489afe303414d42021-11-14T06:37:26ZExpression of Matrix Metalloproteinase-9 in Different Histopathological Variants of Ameloblastoma: A Cross-sectional Study10.7860/JCDR/2021/49261.154232249-782X0973-709Xhttps://doaj.org/article/b9a5af65335b440491489afe303414d42021-09-01T00:00:00Zhttps://www.jcdr.net/articles/PDF/15423/49261_CE[Ra1]_F[SH]_PF1(PS_SHU)_PN(KM).pdfhttps://doaj.org/toc/2249-782Xhttps://doaj.org/toc/0973-709XIntroduction: Ameloblastomas are benign and the most common odontogenic neoplasms with many histopathological subtypes depending on the predominant pattern. They are known for their aggressive behaviour. As ameloblastomas have a high rate of recurrence, it is necessary to understand the biological behaviour of these neoplasms. Matrix Metalloproteinase-9 (MMP-9) is an enzyme that belongs to Metalloproteinases family and is known to degrade the Extracellular Matrix (ECM) and facilitate tumour progression. Evaluation of the expression of MMP-9 in ameloblastomas could contribute in understanding its biological behaviour. Aim: To analyse the expression of MMP-9 in different histopathological variants of ameloblastoma. Materials and Methods: A cross-sectional observational study was done in the Department of Oral Pathology and Microbiology, Bharati Vidyapeeth Deemed to be University, Dental College and Hospital, Pune, India. A total of 30 cases were selected for this study. The study was carried out for a duration of two years from April 2018 to April 2020. The MMP-9 expression was studied by immunohistochemical staining. The statistical comparison was done using Chi-square test between groups, p-value <0.05 was considered significant. The data was statistically analysed using Statistical Package for Social Sciences (SPSS) version 22.0, IBM Corporation, USA for MS Windows. Results: A total of 30 paraffin embedded archival tissue blocks were selected for this study. Among them nine cases were of Plexiform Ameloblastoma, eight of Unicystic Ameloblastoma, three of Acanthomatous Ameloblastoma, four of Desmoplastic Ameloblastoma and six were of Follicular Ameloblastomas. The MMP-9 showed variable expression in different histopathological subtypes of ameloblastoma. This difference was statistically significant between Plexiform and Acanthomatous as well as Plexiform and Follicular variants (p<0.05). A 66.7% (6 out of 9 samples) of Plexiform Ameloblastoma showed intense staining for MMP-9. Conclusion: Expression of MMP-9 varies in different histopathological variants of ameloblastoma and may not have an association with biological behaviour and aggressiveness.Revati Shailesh DeshmukhPriya Nimish DeoSurekha Laxman ChavanPrasad KangoJCDR Research and Publications Private LimitedarticlefollicularimmuohistochemistryplexiformMedicineRENJournal of Clinical and Diagnostic Research, Vol 15, Iss 9, Pp ZC47-ZC50 (2021) |
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follicular immuohistochemistry plexiform Medicine R |
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follicular immuohistochemistry plexiform Medicine R Revati Shailesh Deshmukh Priya Nimish Deo Surekha Laxman Chavan Prasad Kango Expression of Matrix Metalloproteinase-9 in Different Histopathological Variants of Ameloblastoma: A Cross-sectional Study |
| description |
Introduction: Ameloblastomas are benign and the most common
odontogenic neoplasms with many histopathological subtypes
depending on the predominant pattern. They are known for their
aggressive behaviour. As ameloblastomas have a high rate of
recurrence, it is necessary to understand the biological behaviour
of these neoplasms. Matrix Metalloproteinase-9 (MMP-9) is
an enzyme that belongs to Metalloproteinases family and is
known to degrade the Extracellular Matrix (ECM) and facilitate
tumour progression. Evaluation of the expression of MMP-9 in
ameloblastomas could contribute in understanding its biological
behaviour.
Aim: To analyse the expression of MMP-9 in different
histopathological variants of ameloblastoma.
Materials and Methods: A cross-sectional observational study
was done in the Department of Oral Pathology and Microbiology,
Bharati Vidyapeeth Deemed to be University, Dental College and
Hospital, Pune, India. A total of 30 cases were selected for this
study. The study was carried out for a duration of two years from
April 2018 to April 2020. The MMP-9 expression was studied
by immunohistochemical staining. The statistical comparison
was done using Chi-square test between groups, p-value <0.05
was considered significant. The data was statistically analysed
using Statistical Package for Social Sciences (SPSS) version
22.0, IBM Corporation, USA for MS Windows.
Results: A total of 30 paraffin embedded archival tissue blocks
were selected for this study. Among them nine cases were of
Plexiform Ameloblastoma, eight of Unicystic Ameloblastoma,
three of Acanthomatous Ameloblastoma, four of Desmoplastic
Ameloblastoma and six were of Follicular Ameloblastomas. The
MMP-9 showed variable expression in different histopathological
subtypes of ameloblastoma. This difference was statistically
significant between Plexiform and Acanthomatous as well as
Plexiform and Follicular variants (p<0.05). A 66.7% (6 out of 9
samples) of Plexiform Ameloblastoma showed intense staining
for MMP-9.
Conclusion: Expression of MMP-9 varies in different
histopathological variants of ameloblastoma and may not have
an association with biological behaviour and aggressiveness. |
| format |
article |
| author |
Revati Shailesh Deshmukh Priya Nimish Deo Surekha Laxman Chavan Prasad Kango |
| author_facet |
Revati Shailesh Deshmukh Priya Nimish Deo Surekha Laxman Chavan Prasad Kango |
| author_sort |
Revati Shailesh Deshmukh |
| title |
Expression of Matrix Metalloproteinase-9 in Different Histopathological Variants of Ameloblastoma: A Cross-sectional Study |
| title_short |
Expression of Matrix Metalloproteinase-9 in Different Histopathological Variants of Ameloblastoma: A Cross-sectional Study |
| title_full |
Expression of Matrix Metalloproteinase-9 in Different Histopathological Variants of Ameloblastoma: A Cross-sectional Study |
| title_fullStr |
Expression of Matrix Metalloproteinase-9 in Different Histopathological Variants of Ameloblastoma: A Cross-sectional Study |
| title_full_unstemmed |
Expression of Matrix Metalloproteinase-9 in Different Histopathological Variants of Ameloblastoma: A Cross-sectional Study |
| title_sort |
expression of matrix metalloproteinase-9 in different histopathological variants of ameloblastoma: a cross-sectional study |
| publisher |
JCDR Research and Publications Private Limited |
| publishDate |
2021 |
| url |
https://doaj.org/article/b9a5af65335b440491489afe303414d4 |
| work_keys_str_mv |
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| _version_ |
1718429862562103296 |