Leptin and leptin receptor evaluation in atherosclerotic plaques in patients with type 2 diabetes mellitus

Background. Diabetes mellitus (DM) is a significant predictor of atherosclerosis, cardiovascular disease, and cardiovascular mortality. It is known that atherosclerosis occurs earlier in patients with diabetes, reducing the duration of their life. Leptin as well as other inflammatory markers can con...

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Autores principales:	Diana A. Dimitrova, Ilya A. Mikhailov, Konstantin Yu. Tokarev, Marina S. Michurova, Anna M. Gorbacheva, Natalia V. Danilova, Pavel G. Malkov, Viktor Yu. Kalashnikov
Formato:	article
Lenguaje:	RU
Publicado:	"Consilium Medicum" Publishing house 2021
Materias:	type 2 diabetes mellitus atherosclerosis leptin leptin receptor atherosclerotic plaque Medicine R
Acceso en línea:	https://doaj.org/article/b9b83ab25c1c4a9e8613d75ecec68992
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spelling	oai:doaj.org-article:b9b83ab25c1c4a9e8613d75ecec689922021-12-01T12:22:36ZLeptin and leptin receptor evaluation in atherosclerotic plaques in patients with type 2 diabetes mellitus0040-36602309-534210.26442/00403660.2021.10.201079https://doaj.org/article/b9b83ab25c1c4a9e8613d75ecec689922021-10-01T00:00:00Zhttps://ter-arkhiv.ru/0040-3660/article/viewFile/86945/65130https://doaj.org/toc/0040-3660https://doaj.org/toc/2309-5342Background. Diabetes mellitus (DM) is a significant predictor of atherosclerosis, cardiovascular disease, and cardiovascular mortality. It is known that atherosclerosis occurs earlier in patients with diabetes, reducing the duration of their life. Leptin as well as other inflammatory markers can contribute to the progression of atherosclerosis in patients with DM, participate in the development of a local inflammatory reaction. Aim. Determine the cells immunophenotype of atherosclerotic plaques in patients with diabetes. Materials and methods. We analyzed 24 patients (20 men and 4 women), who underwent aortofemoral bypass, femoral-tibial bypass or carotid endarterectomy. During the operation, a fragment of the arterial wall with an atherosclerotic plaque was obtained for further immunohistochemical studies. Five histologic plaque characteristics (CD68+, -SMA, CD34, leptin and leptin receptor) were compared. Results. No difference in the expression of CD68 (p=0.922), -SMA (p=0.192), CD34 (p=0.858), leptin receptor (p=0.741) and leptin (p=0.610) in atherosclerotic plaques were observed between patients with and without DM. The lack of significant differences between the two groups was possibly due to the small number of observations with DM. In particular, when assessing the expression of selected markers in atherosclerotic plaques, patients with DM showed significantly more leptin receptors than patients without DM (2160.716 and 1205.88 respectively); and also significantly less CD68+ (0.39 and 0.98 respectively) and -SMA+ (6.5 and 13.5 respectively). Conclusion. Based on the expression of CD68, -SMA, CD34, leptin receptor and leptin, no significant differences were observed in atherosclerotic plaque between patients with and without DM. At the same time, despite the limitations of the study (a small number of patients, moderate severity of DM, elderly patients in the DM group), we found a tendency in the increased number of leptin receptors and a decreased number of -SMA+, CD68+ in DM atherosclerotic plaques. Further study needed, taking into account the limitations of this work.Diana A. DimitrovaIlya A. MikhailovKonstantin Yu. TokarevMarina S. MichurovaAnna M. GorbachevaNatalia V. DanilovaPavel G. MalkovViktor Yu. Kalashnikov"Consilium Medicum" Publishing housearticletype 2 diabetes mellitusatherosclerosisleptinleptin receptoratherosclerotic plaqueMedicineRRUТерапевтический архив, Vol 93, Iss 10, Pp 1186-1192 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	type 2 diabetes mellitus atherosclerosis leptin leptin receptor atherosclerotic plaque Medicine R
spellingShingle	type 2 diabetes mellitus atherosclerosis leptin leptin receptor atherosclerotic plaque Medicine R Diana A. Dimitrova Ilya A. Mikhailov Konstantin Yu. Tokarev Marina S. Michurova Anna M. Gorbacheva Natalia V. Danilova Pavel G. Malkov Viktor Yu. Kalashnikov Leptin and leptin receptor evaluation in atherosclerotic plaques in patients with type 2 diabetes mellitus
description	Background. Diabetes mellitus (DM) is a significant predictor of atherosclerosis, cardiovascular disease, and cardiovascular mortality. It is known that atherosclerosis occurs earlier in patients with diabetes, reducing the duration of their life. Leptin as well as other inflammatory markers can contribute to the progression of atherosclerosis in patients with DM, participate in the development of a local inflammatory reaction. Aim. Determine the cells immunophenotype of atherosclerotic plaques in patients with diabetes. Materials and methods. We analyzed 24 patients (20 men and 4 women), who underwent aortofemoral bypass, femoral-tibial bypass or carotid endarterectomy. During the operation, a fragment of the arterial wall with an atherosclerotic plaque was obtained for further immunohistochemical studies. Five histologic plaque characteristics (CD68+, -SMA, CD34, leptin and leptin receptor) were compared. Results. No difference in the expression of CD68 (p=0.922), -SMA (p=0.192), CD34 (p=0.858), leptin receptor (p=0.741) and leptin (p=0.610) in atherosclerotic plaques were observed between patients with and without DM. The lack of significant differences between the two groups was possibly due to the small number of observations with DM. In particular, when assessing the expression of selected markers in atherosclerotic plaques, patients with DM showed significantly more leptin receptors than patients without DM (2160.716 and 1205.88 respectively); and also significantly less CD68+ (0.39 and 0.98 respectively) and -SMA+ (6.5 and 13.5 respectively). Conclusion. Based on the expression of CD68, -SMA, CD34, leptin receptor and leptin, no significant differences were observed in atherosclerotic plaque between patients with and without DM. At the same time, despite the limitations of the study (a small number of patients, moderate severity of DM, elderly patients in the DM group), we found a tendency in the increased number of leptin receptors and a decreased number of -SMA+, CD68+ in DM atherosclerotic plaques. Further study needed, taking into account the limitations of this work.
format	article
author	Diana A. Dimitrova Ilya A. Mikhailov Konstantin Yu. Tokarev Marina S. Michurova Anna M. Gorbacheva Natalia V. Danilova Pavel G. Malkov Viktor Yu. Kalashnikov
author_facet	Diana A. Dimitrova Ilya A. Mikhailov Konstantin Yu. Tokarev Marina S. Michurova Anna M. Gorbacheva Natalia V. Danilova Pavel G. Malkov Viktor Yu. Kalashnikov
author_sort	Diana A. Dimitrova
title	Leptin and leptin receptor evaluation in atherosclerotic plaques in patients with type 2 diabetes mellitus
title_short	Leptin and leptin receptor evaluation in atherosclerotic plaques in patients with type 2 diabetes mellitus
title_full	Leptin and leptin receptor evaluation in atherosclerotic plaques in patients with type 2 diabetes mellitus
title_fullStr	Leptin and leptin receptor evaluation in atherosclerotic plaques in patients with type 2 diabetes mellitus
title_full_unstemmed	Leptin and leptin receptor evaluation in atherosclerotic plaques in patients with type 2 diabetes mellitus
title_sort	leptin and leptin receptor evaluation in atherosclerotic plaques in patients with type 2 diabetes mellitus
publisher	"Consilium Medicum" Publishing house
publishDate	2021
url	https://doaj.org/article/b9b83ab25c1c4a9e8613d75ecec68992
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Leptin and leptin receptor evaluation in atherosclerotic plaques in patients with type 2 diabetes mellitus

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