Investigation of proteins important for microcirculation using in vivo microdialysis after glucose provocation: a proteomic study

Abstract Insulin has metabolic and vascular effects in the human body. What mechanisms that orchestrate the effects in the microcirculation, and how the responds differ in different tissues, is however not fully understood. It is therefore of interest to search for markers in microdialysate that may...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alexandra Högstedt, Simon Farnebo, Erik Tesselaar, Bijar Ghafouri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b9be080a6b8840b59ac22cb6483d71f1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b9be080a6b8840b59ac22cb6483d71f1
record_format	dspace
spelling	oai:doaj.org-article:b9be080a6b8840b59ac22cb6483d71f12021-12-02T17:37:12ZInvestigation of proteins important for microcirculation using in vivo microdialysis after glucose provocation: a proteomic study10.1038/s41598-021-98672-82045-2322https://doaj.org/article/b9be080a6b8840b59ac22cb6483d71f12021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98672-8https://doaj.org/toc/2045-2322Abstract Insulin has metabolic and vascular effects in the human body. What mechanisms that orchestrate the effects in the microcirculation, and how the responds differ in different tissues, is however not fully understood. It is therefore of interest to search for markers in microdialysate that may be related to the microcirculation. This study aims to identify proteins related to microvascular changes in different tissue compartments after glucose provocation using in vivo microdialysis. Microdialysis was conducted in three different tissue compartments (intracutaneous, subcutaneous and intravenous) from healthy subjects. Microdialysate was collected during three time periods; recovery after catheter insertion, baseline and glucose provocation, and analyzed using proteomics. Altogether, 126 proteins were detected. Multivariate data analysis showed that the differences in protein expression levels during the three time periods, including comparison before and after glucose provocation, were most pronounced in the intracutaneous and subcutaneous compartments. Four proteins with vascular effects were identified (angiotensinogen, kininogen-1, alpha-2-HS-glycoprotein and hemoglobin subunit beta), all upregulated after glucose provocation compared to baseline in all three compartments. Glucose provocation is known to cause insulin-induced vasodilation through the nitric oxide pathway, and this study indicates that this is facilitated through the interactions of the RAS (angiotensinogen) and kallikrein-kinin (kininogen-1) systems.Alexandra HögstedtSimon FarneboErik TesselaarBijar GhafouriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexandra Högstedt Simon Farnebo Erik Tesselaar Bijar Ghafouri Investigation of proteins important for microcirculation using in vivo microdialysis after glucose provocation: a proteomic study
description	Abstract Insulin has metabolic and vascular effects in the human body. What mechanisms that orchestrate the effects in the microcirculation, and how the responds differ in different tissues, is however not fully understood. It is therefore of interest to search for markers in microdialysate that may be related to the microcirculation. This study aims to identify proteins related to microvascular changes in different tissue compartments after glucose provocation using in vivo microdialysis. Microdialysis was conducted in three different tissue compartments (intracutaneous, subcutaneous and intravenous) from healthy subjects. Microdialysate was collected during three time periods; recovery after catheter insertion, baseline and glucose provocation, and analyzed using proteomics. Altogether, 126 proteins were detected. Multivariate data analysis showed that the differences in protein expression levels during the three time periods, including comparison before and after glucose provocation, were most pronounced in the intracutaneous and subcutaneous compartments. Four proteins with vascular effects were identified (angiotensinogen, kininogen-1, alpha-2-HS-glycoprotein and hemoglobin subunit beta), all upregulated after glucose provocation compared to baseline in all three compartments. Glucose provocation is known to cause insulin-induced vasodilation through the nitric oxide pathway, and this study indicates that this is facilitated through the interactions of the RAS (angiotensinogen) and kallikrein-kinin (kininogen-1) systems.
format	article
author	Alexandra Högstedt Simon Farnebo Erik Tesselaar Bijar Ghafouri
author_facet	Alexandra Högstedt Simon Farnebo Erik Tesselaar Bijar Ghafouri
author_sort	Alexandra Högstedt
title	Investigation of proteins important for microcirculation using in vivo microdialysis after glucose provocation: a proteomic study
title_short	Investigation of proteins important for microcirculation using in vivo microdialysis after glucose provocation: a proteomic study
title_full	Investigation of proteins important for microcirculation using in vivo microdialysis after glucose provocation: a proteomic study
title_fullStr	Investigation of proteins important for microcirculation using in vivo microdialysis after glucose provocation: a proteomic study
title_full_unstemmed	Investigation of proteins important for microcirculation using in vivo microdialysis after glucose provocation: a proteomic study
title_sort	investigation of proteins important for microcirculation using in vivo microdialysis after glucose provocation: a proteomic study
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b9be080a6b8840b59ac22cb6483d71f1
work_keys_str_mv	AT alexandrahogstedt investigationofproteinsimportantformicrocirculationusinginvivomicrodialysisafterglucoseprovocationaproteomicstudy AT simonfarnebo investigationofproteinsimportantformicrocirculationusinginvivomicrodialysisafterglucoseprovocationaproteomicstudy AT eriktesselaar investigationofproteinsimportantformicrocirculationusinginvivomicrodialysisafterglucoseprovocationaproteomicstudy AT bijarghafouri investigationofproteinsimportantformicrocirculationusinginvivomicrodialysisafterglucoseprovocationaproteomicstudy
_version_	1718379905878589440

Investigation of proteins important for microcirculation using in vivo microdialysis after glucose provocation: a proteomic study

Ejemplares similares