Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells

Abstract Plant polyphenol gossypol has anticancer activities. This may increase cottonseed value by using gossypol as a health intervention agent. It is necessary to understand its molecular mechanisms before human consumption. The aim was to uncover the effects of gossypol on cell viability and gen...

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Autores principales:	Heping Cao, Kandan Sethumadhavan, Fangping Cao, Thomas T. Y. Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b9bf3c512c0f43b6a73fa5a5806657a0
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spelling	oai:doaj.org-article:b9bf3c512c0f43b6a73fa5a5806657a02021-12-02T17:05:45ZGossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells10.1038/s41598-021-84970-82045-2322https://doaj.org/article/b9bf3c512c0f43b6a73fa5a5806657a02021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84970-8https://doaj.org/toc/2045-2322Abstract Plant polyphenol gossypol has anticancer activities. This may increase cottonseed value by using gossypol as a health intervention agent. It is necessary to understand its molecular mechanisms before human consumption. The aim was to uncover the effects of gossypol on cell viability and gene expression in cancer cells. In this study, human colon cancer cells (COLO 225) were treated with gossypol. MTT assay showed significant inhibitory effect under high concentration and longtime treatment. We analyzed the expression of 55 genes at the mRNA level in the cells; many of them are regulated by gossypol or ZFP36/TTP in cancer cells. BCL2 mRNA was the most stable among the 55 mRNAs analyzed in human colon cancer cells. GAPDH and RPL32 mRNAs were not good qPCR references for the colon cancer cells. Gossypol decreased the mRNA levels of DGAT, GLUT, TTP, IL families and a number of previously reported genes. In particular, gossypol suppressed the expression of genes coding for CLAUDIN1, ELK1, FAS, GAPDH, IL2, IL8 and ZFAND5 mRNAs, but enhanced the expression of the gene coding for GLUT3 mRNA. The results showed that gossypol inhibited cell survival with decreased expression of a number of genes in the colon cancer cells.Heping CaoKandan SethumadhavanFangping CaoThomas T. Y. WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heping Cao Kandan Sethumadhavan Fangping Cao Thomas T. Y. Wang Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells
description	Abstract Plant polyphenol gossypol has anticancer activities. This may increase cottonseed value by using gossypol as a health intervention agent. It is necessary to understand its molecular mechanisms before human consumption. The aim was to uncover the effects of gossypol on cell viability and gene expression in cancer cells. In this study, human colon cancer cells (COLO 225) were treated with gossypol. MTT assay showed significant inhibitory effect under high concentration and longtime treatment. We analyzed the expression of 55 genes at the mRNA level in the cells; many of them are regulated by gossypol or ZFP36/TTP in cancer cells. BCL2 mRNA was the most stable among the 55 mRNAs analyzed in human colon cancer cells. GAPDH and RPL32 mRNAs were not good qPCR references for the colon cancer cells. Gossypol decreased the mRNA levels of DGAT, GLUT, TTP, IL families and a number of previously reported genes. In particular, gossypol suppressed the expression of genes coding for CLAUDIN1, ELK1, FAS, GAPDH, IL2, IL8 and ZFAND5 mRNAs, but enhanced the expression of the gene coding for GLUT3 mRNA. The results showed that gossypol inhibited cell survival with decreased expression of a number of genes in the colon cancer cells.
format	article
author	Heping Cao Kandan Sethumadhavan Fangping Cao Thomas T. Y. Wang
author_facet	Heping Cao Kandan Sethumadhavan Fangping Cao Thomas T. Y. Wang
author_sort	Heping Cao
title	Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells
title_short	Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells
title_full	Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells
title_fullStr	Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells
title_full_unstemmed	Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells
title_sort	gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b9bf3c512c0f43b6a73fa5a5806657a0
work_keys_str_mv	AT hepingcao gossypoldecreasedcellviabilityanddownregulatedtheexpressionofanumberofgenesinhumancoloncancercells AT kandansethumadhavan gossypoldecreasedcellviabilityanddownregulatedtheexpressionofanumberofgenesinhumancoloncancercells AT fangpingcao gossypoldecreasedcellviabilityanddownregulatedtheexpressionofanumberofgenesinhumancoloncancercells AT thomastywang gossypoldecreasedcellviabilityanddownregulatedtheexpressionofanumberofgenesinhumancoloncancercells
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Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells

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