Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells

Maeda H Mohammad,1 Ahmed M Al-Shammari,1 Ahmad Adnan Al-Juboory,2 Nahi Y Yaseen1 1Experimental Therapy Department, Iraqi Center of Cancer and Medical Genetic Research, Al-Mustansiriyah University, 2Department of Surgery, Neuroscience Hospital, Baghdad, Iraq Abstract: The in vitro isolation, identif...

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Autores principales:	Mohammad MH, Al-Shammari AM, Al-Juboory AA, Yaseen NY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	mesenchymal stem cells neural stem cells NES NF-L MAP-2. Cytology QH573-671
Acceso en línea:	https://doaj.org/article/b9c98ae7739b43d0b88cdf7c4dca1c77
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spelling	oai:doaj.org-article:b9c98ae7739b43d0b88cdf7c4dca1c772021-12-02T01:02:49ZCharacterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells1178-6957https://doaj.org/article/b9c98ae7739b43d0b88cdf7c4dca1c772016-04-01T00:00:00Zhttps://www.dovepress.com/characterization-of-neural-stemness-status-through-the-neurogenesis-pr-peer-reviewed-article-SCCAAhttps://doaj.org/toc/1178-6957Maeda H Mohammad,1 Ahmed M Al-Shammari,1 Ahmad Adnan Al-Juboory,2 Nahi Y Yaseen1 1Experimental Therapy Department, Iraqi Center of Cancer and Medical Genetic Research, Al-Mustansiriyah University, 2Department of Surgery, Neuroscience Hospital, Baghdad, Iraq Abstract: The in vitro isolation, identification, differentiation, and neurogenesis characterization of the sources of mesenchymal stem cells (MSCs) were investigated to produce two types of cells in culture: neural cells and neural stem cells (NSCs). These types of stem cells were used as successful sources for the further treatment of central nervous system defects and injuries. The mouse bone marrow MSCs were used as the source of the stem cells in this study. β-Mercaptoethanol (BME) was used as the main inducer of the neurogenesis pathway to induce neural cells and to identify NSCs. Three types of neural markers were used: nestin as the immaturation stage marker, neurofilament light chain as the early neural marker, and microtubule-associated protein 2 as the maturation marker through different time intervals in the neurogenesis process starting from the MSCs, (as undifferentiated cells), NSCs, production stages, and toward neuron cells (as differentiated cells). The results of different exposure times to BME of the neural markers analysis done by immunocytochemistry and real time-polymerase chain reaction helped us to identify the exact timing for the neural stemness state. The results showed that the best exposure time that may be used for the production of NSCs was 6 hours. The best maintenance media for NSCs were also identified. Furthermore, we optimized exposure to BME with different times and concentrations, which could be an interesting way to modulate specific neuronal differentiation and obtain autologous neuronal phenotypes. This study was able to characterize NSCs in culture under differentiation for neurogenesis in the pathway of the neural differentiation process by studying the expressed neural genes and the ability to maintain these NSCs in culture for further differentiation in thousands of functional neurons for the treatment of brain and spinal cord injuries and defects. Keywords: mesenchymal stem cells, neural stem cells, NES, NF-L, MAP-2Mohammad MHAl-Shammari AMAl-Juboory AAYaseen NYDove Medical Pressarticlemesenchymal stem cellsneural stem cellsNESNF-LMAP-2.CytologyQH573-671ENStem Cells and Cloning: Advances and Applications, Vol 2016, Iss Issue 1, Pp 1-15 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	mesenchymal stem cells neural stem cells NES NF-L MAP-2. Cytology QH573-671
spellingShingle	mesenchymal stem cells neural stem cells NES NF-L MAP-2. Cytology QH573-671 Mohammad MH Al-Shammari AM Al-Juboory AA Yaseen NY Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells
description	Maeda H Mohammad,1 Ahmed M Al-Shammari,1 Ahmad Adnan Al-Juboory,2 Nahi Y Yaseen1 1Experimental Therapy Department, Iraqi Center of Cancer and Medical Genetic Research, Al-Mustansiriyah University, 2Department of Surgery, Neuroscience Hospital, Baghdad, Iraq Abstract: The in vitro isolation, identification, differentiation, and neurogenesis characterization of the sources of mesenchymal stem cells (MSCs) were investigated to produce two types of cells in culture: neural cells and neural stem cells (NSCs). These types of stem cells were used as successful sources for the further treatment of central nervous system defects and injuries. The mouse bone marrow MSCs were used as the source of the stem cells in this study. β-Mercaptoethanol (BME) was used as the main inducer of the neurogenesis pathway to induce neural cells and to identify NSCs. Three types of neural markers were used: nestin as the immaturation stage marker, neurofilament light chain as the early neural marker, and microtubule-associated protein 2 as the maturation marker through different time intervals in the neurogenesis process starting from the MSCs, (as undifferentiated cells), NSCs, production stages, and toward neuron cells (as differentiated cells). The results of different exposure times to BME of the neural markers analysis done by immunocytochemistry and real time-polymerase chain reaction helped us to identify the exact timing for the neural stemness state. The results showed that the best exposure time that may be used for the production of NSCs was 6 hours. The best maintenance media for NSCs were also identified. Furthermore, we optimized exposure to BME with different times and concentrations, which could be an interesting way to modulate specific neuronal differentiation and obtain autologous neuronal phenotypes. This study was able to characterize NSCs in culture under differentiation for neurogenesis in the pathway of the neural differentiation process by studying the expressed neural genes and the ability to maintain these NSCs in culture for further differentiation in thousands of functional neurons for the treatment of brain and spinal cord injuries and defects. Keywords: mesenchymal stem cells, neural stem cells, NES, NF-L, MAP-2
format	article
author	Mohammad MH Al-Shammari AM Al-Juboory AA Yaseen NY
author_facet	Mohammad MH Al-Shammari AM Al-Juboory AA Yaseen NY
author_sort	Mohammad MH
title	Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells
title_short	Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells
title_full	Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells
title_fullStr	Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells
title_full_unstemmed	Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells
title_sort	characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/b9c98ae7739b43d0b88cdf7c4dca1c77
work_keys_str_mv	AT mohammadmh characterizationofneuralstemnessstatusthroughtheneurogenesisprocessforbonemarrowmesenchymalstemcells AT alshammariam characterizationofneuralstemnessstatusthroughtheneurogenesisprocessforbonemarrowmesenchymalstemcells AT aljubooryaa characterizationofneuralstemnessstatusthroughtheneurogenesisprocessforbonemarrowmesenchymalstemcells AT yaseenny characterizationofneuralstemnessstatusthroughtheneurogenesisprocessforbonemarrowmesenchymalstemcells
_version_	1718403340760514560

Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells

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