Two distinct integrin-mediated mechanisms contribute to apical lumen formation in epithelial cells.

<h4>Background</h4>Formation of apical compartments underlies the morphogenesis of most epithelial organs during development. The extracellular matrix (ECM), particularly the basement membrane (BM), plays an important role in orienting the apico-basal polarity and thereby the positioning...

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Autores principales:	Satu Marja Myllymäki, Terhi Piritta Teräväinen, Aki Manninen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b9ca3b83039b4089b04f565d297fa11a
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spelling	oai:doaj.org-article:b9ca3b83039b4089b04f565d297fa11a2021-11-18T06:54:20ZTwo distinct integrin-mediated mechanisms contribute to apical lumen formation in epithelial cells.1932-620310.1371/journal.pone.0019453https://doaj.org/article/b9ca3b83039b4089b04f565d297fa11a2011-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21573123/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Formation of apical compartments underlies the morphogenesis of most epithelial organs during development. The extracellular matrix (ECM), particularly the basement membrane (BM), plays an important role in orienting the apico-basal polarity and thereby the positioning of apical lumens. Integrins have been recognized as essential mediators of matrix-derived polarity signals. The importance of β1-integrins in epithelial polarization is well established but the significance of the accompanying α-subunits have not been analyzed in detail.<h4>Principal findings</h4>Here we demonstrate that two distinct integrin-dependent pathways regulate formation of apical lumens to ensure robust apical membrane biogenesis under different microenvironmental conditions; 1) α2β1- and α6β4-integrins were required to establish a basal cue that depends on Rac1-activity and guides apico-basal cell polarization. 2) α3β1-integrins were implicated in positioning of mitotic spindles in cysts, a process that is essential for Cdc42-driven epithelial hollowing.<h4>Significance</h4>Identification of the separate processes driven by particular integrin receptors clarifies the functional hierarchies between the different integrins co-expressed in epithelial cells and provides valuable insight into the complexity of cell-ECM interactions thereby guiding future studies addressing the molecular basis of epithelial morphogenesis during development and disease.Satu Marja MyllymäkiTerhi Piritta TeräväinenAki ManninenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e19453 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Satu Marja Myllymäki Terhi Piritta Teräväinen Aki Manninen Two distinct integrin-mediated mechanisms contribute to apical lumen formation in epithelial cells.
description	<h4>Background</h4>Formation of apical compartments underlies the morphogenesis of most epithelial organs during development. The extracellular matrix (ECM), particularly the basement membrane (BM), plays an important role in orienting the apico-basal polarity and thereby the positioning of apical lumens. Integrins have been recognized as essential mediators of matrix-derived polarity signals. The importance of β1-integrins in epithelial polarization is well established but the significance of the accompanying α-subunits have not been analyzed in detail.<h4>Principal findings</h4>Here we demonstrate that two distinct integrin-dependent pathways regulate formation of apical lumens to ensure robust apical membrane biogenesis under different microenvironmental conditions; 1) α2β1- and α6β4-integrins were required to establish a basal cue that depends on Rac1-activity and guides apico-basal cell polarization. 2) α3β1-integrins were implicated in positioning of mitotic spindles in cysts, a process that is essential for Cdc42-driven epithelial hollowing.<h4>Significance</h4>Identification of the separate processes driven by particular integrin receptors clarifies the functional hierarchies between the different integrins co-expressed in epithelial cells and provides valuable insight into the complexity of cell-ECM interactions thereby guiding future studies addressing the molecular basis of epithelial morphogenesis during development and disease.
format	article
author	Satu Marja Myllymäki Terhi Piritta Teräväinen Aki Manninen
author_facet	Satu Marja Myllymäki Terhi Piritta Teräväinen Aki Manninen
author_sort	Satu Marja Myllymäki
title	Two distinct integrin-mediated mechanisms contribute to apical lumen formation in epithelial cells.
title_short	Two distinct integrin-mediated mechanisms contribute to apical lumen formation in epithelial cells.
title_full	Two distinct integrin-mediated mechanisms contribute to apical lumen formation in epithelial cells.
title_fullStr	Two distinct integrin-mediated mechanisms contribute to apical lumen formation in epithelial cells.
title_full_unstemmed	Two distinct integrin-mediated mechanisms contribute to apical lumen formation in epithelial cells.
title_sort	two distinct integrin-mediated mechanisms contribute to apical lumen formation in epithelial cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/b9ca3b83039b4089b04f565d297fa11a
work_keys_str_mv	AT satumarjamyllymaki twodistinctintegrinmediatedmechanismscontributetoapicallumenformationinepithelialcells AT terhipirittateravainen twodistinctintegrinmediatedmechanismscontributetoapicallumenformationinepithelialcells AT akimanninen twodistinctintegrinmediatedmechanismscontributetoapicallumenformationinepithelialcells
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Two distinct integrin-mediated mechanisms contribute to apical lumen formation in epithelial cells.

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