Latent transforming growth factor β-binding protein 4 is downregulated in esophageal cancer via promoter methylation.

Latent transforming growth factor β-binding protein 4 (LTBP4) is an extracellular matrix molecule that is a member of important connective tissue networks and is needed for the correct folding and the secretion of TGF-β1. LTBP4 is downregulated in carcinomas of various tissues. Here we show that LTB...

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Autores principales:	Insa Bultmann, Anne Conradi, Celine Kretschmer, Anja Sterner-Kock
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b9ce7702860649d8ba8054f2bd047482
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spelling	oai:doaj.org-article:b9ce7702860649d8ba8054f2bd0474822021-11-18T07:43:23ZLatent transforming growth factor β-binding protein 4 is downregulated in esophageal cancer via promoter methylation.1932-620310.1371/journal.pone.0065614https://doaj.org/article/b9ce7702860649d8ba8054f2bd0474822013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23741501/?tool=EBIhttps://doaj.org/toc/1932-6203Latent transforming growth factor β-binding protein 4 (LTBP4) is an extracellular matrix molecule that is a member of important connective tissue networks and is needed for the correct folding and the secretion of TGF-β1. LTBP4 is downregulated in carcinomas of various tissues. Here we show that LTBP4 is also downregulated in adenocarcinomas and squamous cell carcinomas of the esophagus in vitro and in vivo. Re-expression of LTBP4 in esophageal cancer cell lines reduced cell migration ability, whereas cell viability and cell proliferation remained unchanged. Hypermethylation of the promoter regions of the two main human LTBP4 transcriptional forms, LTBP4L and LTBP4S, was found to be involved in LTBP4 silencing. Detailed investigations of the methylation patterns of the promoter regions of LTBP4L and LTBP4S identified GATA1, SP1, E2F4 and SMAD3 as potential transcription factors involved in LTBP4 expression. In in vitro transcription factor activity studies we discovered E2F4 as novel powerful regulator for LTBP4S expression.Insa BultmannAnne ConradiCeline KretschmerAnja Sterner-KockPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e65614 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Insa Bultmann Anne Conradi Celine Kretschmer Anja Sterner-Kock Latent transforming growth factor β-binding protein 4 is downregulated in esophageal cancer via promoter methylation.
description	Latent transforming growth factor β-binding protein 4 (LTBP4) is an extracellular matrix molecule that is a member of important connective tissue networks and is needed for the correct folding and the secretion of TGF-β1. LTBP4 is downregulated in carcinomas of various tissues. Here we show that LTBP4 is also downregulated in adenocarcinomas and squamous cell carcinomas of the esophagus in vitro and in vivo. Re-expression of LTBP4 in esophageal cancer cell lines reduced cell migration ability, whereas cell viability and cell proliferation remained unchanged. Hypermethylation of the promoter regions of the two main human LTBP4 transcriptional forms, LTBP4L and LTBP4S, was found to be involved in LTBP4 silencing. Detailed investigations of the methylation patterns of the promoter regions of LTBP4L and LTBP4S identified GATA1, SP1, E2F4 and SMAD3 as potential transcription factors involved in LTBP4 expression. In in vitro transcription factor activity studies we discovered E2F4 as novel powerful regulator for LTBP4S expression.
format	article
author	Insa Bultmann Anne Conradi Celine Kretschmer Anja Sterner-Kock
author_facet	Insa Bultmann Anne Conradi Celine Kretschmer Anja Sterner-Kock
author_sort	Insa Bultmann
title	Latent transforming growth factor β-binding protein 4 is downregulated in esophageal cancer via promoter methylation.
title_short	Latent transforming growth factor β-binding protein 4 is downregulated in esophageal cancer via promoter methylation.
title_full	Latent transforming growth factor β-binding protein 4 is downregulated in esophageal cancer via promoter methylation.
title_fullStr	Latent transforming growth factor β-binding protein 4 is downregulated in esophageal cancer via promoter methylation.
title_full_unstemmed	Latent transforming growth factor β-binding protein 4 is downregulated in esophageal cancer via promoter methylation.
title_sort	latent transforming growth factor β-binding protein 4 is downregulated in esophageal cancer via promoter methylation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b9ce7702860649d8ba8054f2bd047482
work_keys_str_mv	AT insabultmann latenttransforminggrowthfactorbbindingprotein4isdownregulatedinesophagealcancerviapromotermethylation AT anneconradi latenttransforminggrowthfactorbbindingprotein4isdownregulatedinesophagealcancerviapromotermethylation AT celinekretschmer latenttransforminggrowthfactorbbindingprotein4isdownregulatedinesophagealcancerviapromotermethylation AT anjasternerkock latenttransforminggrowthfactorbbindingprotein4isdownregulatedinesophagealcancerviapromotermethylation
_version_	1718423058465685504

Latent transforming growth factor β-binding protein 4 is downregulated in esophageal cancer via promoter methylation.

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