Phenotypic characterization of retinoic acid differentiated SH-SY5Y cells by transcriptional profiling.

Multiple genetic and environmental factors play a role in the development and progression of Parkinson's disease (PD). The main neuropathological hallmark of PD is the degeneration of dopaminergic (DAergic) neurons in the substantia nigra pars compacta. To study genetic and molecular contributo...

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Autores principales:	Joanna A Korecka, Ronald E van Kesteren, Eva Blaas, Sonia O Spitzer, Jorke H Kamstra, August B Smit, Dick F Swaab, Joost Verhaagen, Koen Bossers
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b9d57f6d28de438980edd24634c769d1
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spelling	oai:doaj.org-article:b9d57f6d28de438980edd24634c769d12021-11-18T07:44:10ZPhenotypic characterization of retinoic acid differentiated SH-SY5Y cells by transcriptional profiling.1932-620310.1371/journal.pone.0063862https://doaj.org/article/b9d57f6d28de438980edd24634c769d12013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23724009/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Multiple genetic and environmental factors play a role in the development and progression of Parkinson's disease (PD). The main neuropathological hallmark of PD is the degeneration of dopaminergic (DAergic) neurons in the substantia nigra pars compacta. To study genetic and molecular contributors to the disease process, there is a great need for readily accessible cells with prominent DAergic features that can be used for reproducible in vitro cellular screening. Here, we investigated the molecular phenotype of retinoic acid (RA) differentiated SH-SY5Y cells using genome wide transcriptional profiling combined with gene ontology, transcription factor and molecular pathway analysis. We demonstrated that RA induces a general neuronal differentiation program in SH-SY5Y cells and that these cells develop a predominantly mature DAergic-like neurotransmitter phenotype. This phenotype is characterized by increased dopamine levels together with a substantial suppression of other neurotransmitter phenotypes, such as those for noradrenaline, acetylcholine, glutamate, serotonin and histamine. In addition, we show that RA differentiated SH-SY5Y cells express the dopamine and noradrenalin neurotransmitter transporters that are responsible for uptake of MPP(+), a well known DAergic cell toxicant. MPP(+) treatment alters mitochondrial activity according to its proposed cytotoxic effect in DAergic neurons. Taken together, RA differentiated SH-SY5Y cells have a DAergic-like phenotype, and provide a good cellular screening tool to find novel genes or compounds that affect cytotoxic processes that are associated with PD.Joanna A KoreckaRonald E van KesterenEva BlaasSonia O SpitzerJorke H KamstraAugust B SmitDick F SwaabJoost VerhaagenKoen BossersPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63862 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joanna A Korecka Ronald E van Kesteren Eva Blaas Sonia O Spitzer Jorke H Kamstra August B Smit Dick F Swaab Joost Verhaagen Koen Bossers Phenotypic characterization of retinoic acid differentiated SH-SY5Y cells by transcriptional profiling.
description	Multiple genetic and environmental factors play a role in the development and progression of Parkinson's disease (PD). The main neuropathological hallmark of PD is the degeneration of dopaminergic (DAergic) neurons in the substantia nigra pars compacta. To study genetic and molecular contributors to the disease process, there is a great need for readily accessible cells with prominent DAergic features that can be used for reproducible in vitro cellular screening. Here, we investigated the molecular phenotype of retinoic acid (RA) differentiated SH-SY5Y cells using genome wide transcriptional profiling combined with gene ontology, transcription factor and molecular pathway analysis. We demonstrated that RA induces a general neuronal differentiation program in SH-SY5Y cells and that these cells develop a predominantly mature DAergic-like neurotransmitter phenotype. This phenotype is characterized by increased dopamine levels together with a substantial suppression of other neurotransmitter phenotypes, such as those for noradrenaline, acetylcholine, glutamate, serotonin and histamine. In addition, we show that RA differentiated SH-SY5Y cells express the dopamine and noradrenalin neurotransmitter transporters that are responsible for uptake of MPP(+), a well known DAergic cell toxicant. MPP(+) treatment alters mitochondrial activity according to its proposed cytotoxic effect in DAergic neurons. Taken together, RA differentiated SH-SY5Y cells have a DAergic-like phenotype, and provide a good cellular screening tool to find novel genes or compounds that affect cytotoxic processes that are associated with PD.
format	article
author	Joanna A Korecka Ronald E van Kesteren Eva Blaas Sonia O Spitzer Jorke H Kamstra August B Smit Dick F Swaab Joost Verhaagen Koen Bossers
author_facet	Joanna A Korecka Ronald E van Kesteren Eva Blaas Sonia O Spitzer Jorke H Kamstra August B Smit Dick F Swaab Joost Verhaagen Koen Bossers
author_sort	Joanna A Korecka
title	Phenotypic characterization of retinoic acid differentiated SH-SY5Y cells by transcriptional profiling.
title_short	Phenotypic characterization of retinoic acid differentiated SH-SY5Y cells by transcriptional profiling.
title_full	Phenotypic characterization of retinoic acid differentiated SH-SY5Y cells by transcriptional profiling.
title_fullStr	Phenotypic characterization of retinoic acid differentiated SH-SY5Y cells by transcriptional profiling.
title_full_unstemmed	Phenotypic characterization of retinoic acid differentiated SH-SY5Y cells by transcriptional profiling.
title_sort	phenotypic characterization of retinoic acid differentiated sh-sy5y cells by transcriptional profiling.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b9d57f6d28de438980edd24634c769d1
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Phenotypic characterization of retinoic acid differentiated SH-SY5Y cells by transcriptional profiling.

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