Green Synthesis of Zinc Oxide Nanoparticles (ZnO-NPs) by <i>Pseudomonas aeruginosa</i> and Their Activity against Pathogenic Microbes and Common House Mosquito, <i>Culex pipiens</i>
The synthesis of nanoparticles by green approaches is gaining unique importance due to its low cost, biocompatibility, high productivity, and purity, and being environmentally friendly. Herein, biomass filtrate of <i>Pseudomonas aeruginosa</i> isolated from mangrove rhizosphere sediment...
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| Autores principales: | , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/b9d97e24a03a4eb79812732a827a4355 |
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| Sumario: | The synthesis of nanoparticles by green approaches is gaining unique importance due to its low cost, biocompatibility, high productivity, and purity, and being environmentally friendly. Herein, biomass filtrate of <i>Pseudomonas aeruginosa</i> isolated from mangrove rhizosphere sediment was used for the biosynthesis of zinc oxide nanoparticles (ZnO-NPs). The bacterial isolate was identified based on morphological, physiological, and 16S rRNA. The bio-fabricated ZnO-NPs were characterized using color change, UV-visible spectroscopy, FT-IR, TEM, and XRD analyses. In the current study, spherical and crystalline nature ZnO-NPs were successfully formed at a maximum SPR (surface plasmon resonance) of 380 nm. The bioactivities of fabricated ZnO-NPs including antibacterial, anti-<i>candida</i>, and larvicidal efficacy were investigated. Data analysis showed that these bioactivities were concentration-dependent. The green-synthesized ZnO-NPs exhibited high efficacy against pathogenic Gram-positive bacteria (<i>Staphylococcus aureus</i> and <i>Bacillus subtilis</i>), Gram-negative bacteria (<i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i>), and unicellular fungi (<i>Candida albicans</i>) with inhibition zones of (12.33 ± 0.9 and 29.3 ± 0.3 mm), (19.3 ± 0.3 and 11.7 ± 0.3 mm), and (22.3 ± 0.3 mm), respectively, at 200 ppm. The MIC value was detected as 50 ppm for <i>E. coli</i>, <i>B. subtilis</i>, and <i>C. albicans</i>, and 200 ppm for <i>S. aureus</i> and <i>P. aeruginosa</i> with zones of inhibition ranging between 11.7 ± 0.3–14.6 ± 0.6 mm. Moreover, the biosynthesized ZnO-NPs showed high mortality for <i>Culex pipiens</i> with percentages of 100 ± 0.0% at 200 ppm after 24 h as compared with zinc acetate (44.3 ± 3.3%) at the same concentration and the same time. |
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