Green Synthesis of Zinc Oxide Nanoparticles (ZnO-NPs) by Pseudomonas aeruginosa and Their Activity against Pathogenic Microbes and Common House Mosquito, Culex pipiens

The synthesis of nanoparticles by green approaches is gaining unique importance due to its low cost, biocompatibility, high productivity, and purity, and being environmentally friendly. Herein, biomass filtrate of Pseudomonas aeruginosa isolated from mangrove rhizosphere sediment...

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Autores principales:	Abdullah M. Abdo, Amr Fouda, Ahmed M. Eid, Nayer M. Fahmy, Ahmed M. Elsayed, Ahmed Mohamed Aly Khalil, Othman M. Alzahrani, Atef F. Ahmed, Amal M. Soliman
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	green synthesis ZnO-NPs <i>Pseudomonas aeruginosa</i> antimicrobial activity larvicidal activity Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
Acceso en línea:	https://doaj.org/article/b9d97e24a03a4eb79812732a827a4355
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spelling	oai:doaj.org-article:b9d97e24a03a4eb79812732a827a43552021-11-25T18:15:26ZGreen Synthesis of Zinc Oxide Nanoparticles (ZnO-NPs) by <i>Pseudomonas aeruginosa</i> and Their Activity against Pathogenic Microbes and Common House Mosquito, <i>Culex pipiens</i>10.3390/ma142269831996-1944https://doaj.org/article/b9d97e24a03a4eb79812732a827a43552021-11-01T00:00:00Zhttps://www.mdpi.com/1996-1944/14/22/6983https://doaj.org/toc/1996-1944The synthesis of nanoparticles by green approaches is gaining unique importance due to its low cost, biocompatibility, high productivity, and purity, and being environmentally friendly. Herein, biomass filtrate of <i>Pseudomonas aeruginosa</i> isolated from mangrove rhizosphere sediment was used for the biosynthesis of zinc oxide nanoparticles (ZnO-NPs). The bacterial isolate was identified based on morphological, physiological, and 16S rRNA. The bio-fabricated ZnO-NPs were characterized using color change, UV-visible spectroscopy, FT-IR, TEM, and XRD analyses. In the current study, spherical and crystalline nature ZnO-NPs were successfully formed at a maximum SPR (surface plasmon resonance) of 380 nm. The bioactivities of fabricated ZnO-NPs including antibacterial, anti-<i>candida</i>, and larvicidal efficacy were investigated. Data analysis showed that these bioactivities were concentration-dependent. The green-synthesized ZnO-NPs exhibited high efficacy against pathogenic Gram-positive bacteria (<i>Staphylococcus aureus</i> and <i>Bacillus subtilis</i>), Gram-negative bacteria (<i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i>), and unicellular fungi (<i>Candida albicans</i>) with inhibition zones of (12.33 ± 0.9 and 29.3 ± 0.3 mm), (19.3 ± 0.3 and 11.7 ± 0.3 mm), and (22.3 ± 0.3 mm), respectively, at 200 ppm. The MIC value was detected as 50 ppm for <i>E. coli</i>, <i>B. subtilis</i>, and <i>C. albicans</i>, and 200 ppm for <i>S. aureus</i> and <i>P. aeruginosa</i> with zones of inhibition ranging between 11.7 ± 0.3–14.6 ± 0.6 mm. Moreover, the biosynthesized ZnO-NPs showed high mortality for <i>Culex pipiens</i> with percentages of 100 ± 0.0% at 200 ppm after 24 h as compared with zinc acetate (44.3 ± 3.3%) at the same concentration and the same time.Abdullah M. AbdoAmr FoudaAhmed M. EidNayer M. FahmyAhmed M. ElsayedAhmed Mohamed Aly KhalilOthman M. AlzahraniAtef F. AhmedAmal M. SolimanMDPI AGarticlegreen synthesisZnO-NPs<i>Pseudomonas aeruginosa</i>antimicrobial activitylarvicidal activityTechnologyTElectrical engineering. Electronics. Nuclear engineeringTK1-9971Engineering (General). Civil engineering (General)TA1-2040MicroscopyQH201-278.5Descriptive and experimental mechanicsQC120-168.85ENMaterials, Vol 14, Iss 6983, p 6983 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	green synthesis ZnO-NPs <i>Pseudomonas aeruginosa</i> antimicrobial activity larvicidal activity Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
spellingShingle	green synthesis ZnO-NPs <i>Pseudomonas aeruginosa</i> antimicrobial activity larvicidal activity Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 Abdullah M. Abdo Amr Fouda Ahmed M. Eid Nayer M. Fahmy Ahmed M. Elsayed Ahmed Mohamed Aly Khalil Othman M. Alzahrani Atef F. Ahmed Amal M. Soliman Green Synthesis of Zinc Oxide Nanoparticles (ZnO-NPs) by <i>Pseudomonas aeruginosa</i> and Their Activity against Pathogenic Microbes and Common House Mosquito, <i>Culex pipiens</i>
description	The synthesis of nanoparticles by green approaches is gaining unique importance due to its low cost, biocompatibility, high productivity, and purity, and being environmentally friendly. Herein, biomass filtrate of <i>Pseudomonas aeruginosa</i> isolated from mangrove rhizosphere sediment was used for the biosynthesis of zinc oxide nanoparticles (ZnO-NPs). The bacterial isolate was identified based on morphological, physiological, and 16S rRNA. The bio-fabricated ZnO-NPs were characterized using color change, UV-visible spectroscopy, FT-IR, TEM, and XRD analyses. In the current study, spherical and crystalline nature ZnO-NPs were successfully formed at a maximum SPR (surface plasmon resonance) of 380 nm. The bioactivities of fabricated ZnO-NPs including antibacterial, anti-<i>candida</i>, and larvicidal efficacy were investigated. Data analysis showed that these bioactivities were concentration-dependent. The green-synthesized ZnO-NPs exhibited high efficacy against pathogenic Gram-positive bacteria (<i>Staphylococcus aureus</i> and <i>Bacillus subtilis</i>), Gram-negative bacteria (<i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i>), and unicellular fungi (<i>Candida albicans</i>) with inhibition zones of (12.33 ± 0.9 and 29.3 ± 0.3 mm), (19.3 ± 0.3 and 11.7 ± 0.3 mm), and (22.3 ± 0.3 mm), respectively, at 200 ppm. The MIC value was detected as 50 ppm for <i>E. coli</i>, <i>B. subtilis</i>, and <i>C. albicans</i>, and 200 ppm for <i>S. aureus</i> and <i>P. aeruginosa</i> with zones of inhibition ranging between 11.7 ± 0.3–14.6 ± 0.6 mm. Moreover, the biosynthesized ZnO-NPs showed high mortality for <i>Culex pipiens</i> with percentages of 100 ± 0.0% at 200 ppm after 24 h as compared with zinc acetate (44.3 ± 3.3%) at the same concentration and the same time.
format	article
author	Abdullah M. Abdo Amr Fouda Ahmed M. Eid Nayer M. Fahmy Ahmed M. Elsayed Ahmed Mohamed Aly Khalil Othman M. Alzahrani Atef F. Ahmed Amal M. Soliman
author_facet	Abdullah M. Abdo Amr Fouda Ahmed M. Eid Nayer M. Fahmy Ahmed M. Elsayed Ahmed Mohamed Aly Khalil Othman M. Alzahrani Atef F. Ahmed Amal M. Soliman
author_sort	Abdullah M. Abdo
title	Green Synthesis of Zinc Oxide Nanoparticles (ZnO-NPs) by <i>Pseudomonas aeruginosa</i> and Their Activity against Pathogenic Microbes and Common House Mosquito, <i>Culex pipiens</i>
title_short	Green Synthesis of Zinc Oxide Nanoparticles (ZnO-NPs) by <i>Pseudomonas aeruginosa</i> and Their Activity against Pathogenic Microbes and Common House Mosquito, <i>Culex pipiens</i>
title_full	Green Synthesis of Zinc Oxide Nanoparticles (ZnO-NPs) by <i>Pseudomonas aeruginosa</i> and Their Activity against Pathogenic Microbes and Common House Mosquito, <i>Culex pipiens</i>
title_fullStr	Green Synthesis of Zinc Oxide Nanoparticles (ZnO-NPs) by <i>Pseudomonas aeruginosa</i> and Their Activity against Pathogenic Microbes and Common House Mosquito, <i>Culex pipiens</i>
title_full_unstemmed	Green Synthesis of Zinc Oxide Nanoparticles (ZnO-NPs) by <i>Pseudomonas aeruginosa</i> and Their Activity against Pathogenic Microbes and Common House Mosquito, <i>Culex pipiens</i>
title_sort	green synthesis of zinc oxide nanoparticles (zno-nps) by <i>pseudomonas aeruginosa</i> and their activity against pathogenic microbes and common house mosquito, <i>culex pipiens</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b9d97e24a03a4eb79812732a827a4355
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Green Synthesis of Zinc Oxide Nanoparticles (ZnO-NPs) by <i>Pseudomonas aeruginosa</i> and Their Activity against Pathogenic Microbes and Common House Mosquito, <i>Culex pipiens</i>

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