Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine

Abstract In this study, by combining nuclear magnetic resonance (NMR), circular dichroism (CD), liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS), and gel electrophoresis, we report an unusual topological structure of the RNA G-quadruplex motif formed by human telomere RNA...

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Autores principales:	Chao-Da Xiao, Takumi Ishizuka, Yan Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b9eaae91570446d59a8f828c23d1461d
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spelling	oai:doaj.org-article:b9eaae91570446d59a8f828c23d1461d2021-12-02T12:32:00ZAntiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine10.1038/s41598-017-07050-w2045-2322https://doaj.org/article/b9eaae91570446d59a8f828c23d1461d2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07050-whttps://doaj.org/toc/2045-2322Abstract In this study, by combining nuclear magnetic resonance (NMR), circular dichroism (CD), liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS), and gel electrophoresis, we report an unusual topological structure of the RNA G-quadruplex motif formed by human telomere RNA r(UAGGGU) containing 8-bromoguanosine. Results showed that the RNA sequence formed an antiparallel tetramolecular G-quadruplex, in which each pair of diagonal strands run in opposite directions. Furthermore, guanosines were observed both in syn- and anti-conformations. In addition, two of these G-quadruplex subunits were found to be stacking on top of each other, forming a dimeric RNA G-quadruplex. Our findings provide a new insight into the behavior of RNA G-quadruplex structures.Chao-Da XiaoTakumi IshizukaYan XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chao-Da Xiao Takumi Ishizuka Yan Xu Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine
description	Abstract In this study, by combining nuclear magnetic resonance (NMR), circular dichroism (CD), liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS), and gel electrophoresis, we report an unusual topological structure of the RNA G-quadruplex motif formed by human telomere RNA r(UAGGGU) containing 8-bromoguanosine. Results showed that the RNA sequence formed an antiparallel tetramolecular G-quadruplex, in which each pair of diagonal strands run in opposite directions. Furthermore, guanosines were observed both in syn- and anti-conformations. In addition, two of these G-quadruplex subunits were found to be stacking on top of each other, forming a dimeric RNA G-quadruplex. Our findings provide a new insight into the behavior of RNA G-quadruplex structures.
format	article
author	Chao-Da Xiao Takumi Ishizuka Yan Xu
author_facet	Chao-Da Xiao Takumi Ishizuka Yan Xu
author_sort	Chao-Da Xiao
title	Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine
title_short	Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine
title_full	Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine
title_fullStr	Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine
title_full_unstemmed	Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine
title_sort	antiparallel rna g-quadruplex formed by human telomere rna containing 8-bromoguanosine
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b9eaae91570446d59a8f828c23d1461d
work_keys_str_mv	AT chaodaxiao antiparallelrnagquadruplexformedbyhumantelomerernacontaining8bromoguanosine AT takumiishizuka antiparallelrnagquadruplexformedbyhumantelomerernacontaining8bromoguanosine AT yanxu antiparallelrnagquadruplexformedbyhumantelomerernacontaining8bromoguanosine
_version_	1718394238106861568

Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine

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