Circadian clock regulation of the cell cycle in the zebrafish intestine.

The circadian clock controls cell proliferation in a number of healthy tissues where cell renewal and regeneration are critical for normal physiological function. The intestine is an organ that typically undergoes regular cycles of cell division, differentiation and apoptosis as part of its role in...

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Autores principales:	Elodie Peyric, Helen A Moore, David Whitmore
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b9f50362c9a24c1798b6b55052afe7e8
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spelling	oai:doaj.org-article:b9f50362c9a24c1798b6b55052afe7e82021-11-18T08:58:04ZCircadian clock regulation of the cell cycle in the zebrafish intestine.1932-620310.1371/journal.pone.0073209https://doaj.org/article/b9f50362c9a24c1798b6b55052afe7e82013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24013905/?tool=EBIhttps://doaj.org/toc/1932-6203The circadian clock controls cell proliferation in a number of healthy tissues where cell renewal and regeneration are critical for normal physiological function. The intestine is an organ that typically undergoes regular cycles of cell division, differentiation and apoptosis as part of its role in digestion and nutrient absorption. The aim of this study was to explore circadian clock regulation of cell proliferation and cell cycle gene expression in the zebrafish intestine. Here we show that the zebrafish gut contains a directly light-entrainable circadian pacemaker, which regulates the daily timing of mitosis. Furthermore, this intestinal clock controls the expression of key cell cycle regulators, such as cdc2, wee1, p21, PCNA and cdk2, but only weakly influences cyclin B1, cyclin B2 and cyclin E1 expression. Interestingly, food deprivation has little impact on circadian clock function in the gut, but dramatically reduces cell proliferation, as well as cell cycle gene expression in this tissue. Timed feeding under constant dark conditions is able to drive rhythmic expression not only of circadian clock genes, but also of several cell cycle genes, suggesting that food can entrain the clock, as well as the cell cycle in the intestine. Rather surprisingly, we found that timed feeding is critical for high amplitude rhythms in cell cycle gene expression, even when zebrafish are maintained on a light-dark cycle. Together these results suggest that the intestinal clock integrates multiple rhythmic cues, including light and food, to function optimally.Elodie PeyricHelen A MooreDavid WhitmorePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e73209 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elodie Peyric Helen A Moore David Whitmore Circadian clock regulation of the cell cycle in the zebrafish intestine.
description	The circadian clock controls cell proliferation in a number of healthy tissues where cell renewal and regeneration are critical for normal physiological function. The intestine is an organ that typically undergoes regular cycles of cell division, differentiation and apoptosis as part of its role in digestion and nutrient absorption. The aim of this study was to explore circadian clock regulation of cell proliferation and cell cycle gene expression in the zebrafish intestine. Here we show that the zebrafish gut contains a directly light-entrainable circadian pacemaker, which regulates the daily timing of mitosis. Furthermore, this intestinal clock controls the expression of key cell cycle regulators, such as cdc2, wee1, p21, PCNA and cdk2, but only weakly influences cyclin B1, cyclin B2 and cyclin E1 expression. Interestingly, food deprivation has little impact on circadian clock function in the gut, but dramatically reduces cell proliferation, as well as cell cycle gene expression in this tissue. Timed feeding under constant dark conditions is able to drive rhythmic expression not only of circadian clock genes, but also of several cell cycle genes, suggesting that food can entrain the clock, as well as the cell cycle in the intestine. Rather surprisingly, we found that timed feeding is critical for high amplitude rhythms in cell cycle gene expression, even when zebrafish are maintained on a light-dark cycle. Together these results suggest that the intestinal clock integrates multiple rhythmic cues, including light and food, to function optimally.
format	article
author	Elodie Peyric Helen A Moore David Whitmore
author_facet	Elodie Peyric Helen A Moore David Whitmore
author_sort	Elodie Peyric
title	Circadian clock regulation of the cell cycle in the zebrafish intestine.
title_short	Circadian clock regulation of the cell cycle in the zebrafish intestine.
title_full	Circadian clock regulation of the cell cycle in the zebrafish intestine.
title_fullStr	Circadian clock regulation of the cell cycle in the zebrafish intestine.
title_full_unstemmed	Circadian clock regulation of the cell cycle in the zebrafish intestine.
title_sort	circadian clock regulation of the cell cycle in the zebrafish intestine.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b9f50362c9a24c1798b6b55052afe7e8
work_keys_str_mv	AT elodiepeyric circadianclockregulationofthecellcycleinthezebrafishintestine AT helenamoore circadianclockregulationofthecellcycleinthezebrafishintestine AT davidwhitmore circadianclockregulationofthecellcycleinthezebrafishintestine
_version_	1718421106171314176

Circadian clock regulation of the cell cycle in the zebrafish intestine.

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