Exosomes mediate LTB4 release during neutrophil chemotaxis.

Leukotriene B4 (LTB4) is secreted by chemotactic neutrophils, forming a secondary gradient that amplifies the reach of primary chemoattractants. This strategy increases the recruitment range for neutrophils and is important during inflammation. Here, we show that LTB4 and its synthesizing enzymes lo...

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Autores principales:	Ritankar Majumdar, Aidin Tavakoli Tameh, Subhash B Arya, Carole A Parent
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b9f81a5a33d24e92bdf7d59c2aca07b8
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spelling	oai:doaj.org-article:b9f81a5a33d24e92bdf7d59c2aca07b82021-12-02T19:54:24ZExosomes mediate LTB4 release during neutrophil chemotaxis.1544-91731545-788510.1371/journal.pbio.3001271https://doaj.org/article/b9f81a5a33d24e92bdf7d59c2aca07b82021-07-01T00:00:00Zhttps://doi.org/10.1371/journal.pbio.3001271https://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Leukotriene B4 (LTB4) is secreted by chemotactic neutrophils, forming a secondary gradient that amplifies the reach of primary chemoattractants. This strategy increases the recruitment range for neutrophils and is important during inflammation. Here, we show that LTB4 and its synthesizing enzymes localize to intracellular multivesicular bodies, which, upon stimulation, release their content as exosomes. Purified exosomes can activate resting neutrophils and elicit chemotactic activity in an LTB4 receptor-dependent manner. Inhibition of exosome release leads to loss of directional motility with concomitant loss of LTB4 release. Our findings establish that the exosomal pool of LTB4 acts in an autocrine fashion to sensitize neutrophils towards the primary chemoattractant, and in a paracrine fashion to mediate the recruitment of neighboring neutrophils in trans. We envision that this mechanism is used by other signals to foster communication between cells in harsh extracellular environments.Ritankar MajumdarAidin Tavakoli TamehSubhash B AryaCarole A ParentPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 19, Iss 7, p e3001271 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Ritankar Majumdar Aidin Tavakoli Tameh Subhash B Arya Carole A Parent Exosomes mediate LTB4 release during neutrophil chemotaxis.
description	Leukotriene B4 (LTB4) is secreted by chemotactic neutrophils, forming a secondary gradient that amplifies the reach of primary chemoattractants. This strategy increases the recruitment range for neutrophils and is important during inflammation. Here, we show that LTB4 and its synthesizing enzymes localize to intracellular multivesicular bodies, which, upon stimulation, release their content as exosomes. Purified exosomes can activate resting neutrophils and elicit chemotactic activity in an LTB4 receptor-dependent manner. Inhibition of exosome release leads to loss of directional motility with concomitant loss of LTB4 release. Our findings establish that the exosomal pool of LTB4 acts in an autocrine fashion to sensitize neutrophils towards the primary chemoattractant, and in a paracrine fashion to mediate the recruitment of neighboring neutrophils in trans. We envision that this mechanism is used by other signals to foster communication between cells in harsh extracellular environments.
format	article
author	Ritankar Majumdar Aidin Tavakoli Tameh Subhash B Arya Carole A Parent
author_facet	Ritankar Majumdar Aidin Tavakoli Tameh Subhash B Arya Carole A Parent
author_sort	Ritankar Majumdar
title	Exosomes mediate LTB4 release during neutrophil chemotaxis.
title_short	Exosomes mediate LTB4 release during neutrophil chemotaxis.
title_full	Exosomes mediate LTB4 release during neutrophil chemotaxis.
title_fullStr	Exosomes mediate LTB4 release during neutrophil chemotaxis.
title_full_unstemmed	Exosomes mediate LTB4 release during neutrophil chemotaxis.
title_sort	exosomes mediate ltb4 release during neutrophil chemotaxis.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/b9f81a5a33d24e92bdf7d59c2aca07b8
work_keys_str_mv	AT ritankarmajumdar exosomesmediateltb4releaseduringneutrophilchemotaxis AT aidintavakolitameh exosomesmediateltb4releaseduringneutrophilchemotaxis AT subhashbarya exosomesmediateltb4releaseduringneutrophilchemotaxis AT caroleaparent exosomesmediateltb4releaseduringneutrophilchemotaxis
_version_	1718375923111165952

Exosomes mediate LTB4 release during neutrophil chemotaxis.

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