Probiotic Diversity Enhances Rhizosphere Microbiome Function and Plant Disease Suppression

ABSTRACT Bacterial communities associated with plant roots play an important role in the suppression of soil-borne pathogens, and multispecies probiotic consortia may enhance disease suppression efficacy. Here we introduced defined Pseudomonas species consortia into naturally complex microbial commu...

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Autores principales:	Jie Hu, Zhong Wei, Ville-Petri Friman, Shao-hua Gu, Xiao-fang Wang, Nico Eisenhauer, Tian-jie Yang, Jing Ma, Qi-rong Shen, Yang-chun Xu, Alexandre Jousset
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b9f8ad852223458c8aee9bf607c783cb
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spelling	oai:doaj.org-article:b9f8ad852223458c8aee9bf607c783cb2021-11-15T15:50:15ZProbiotic Diversity Enhances Rhizosphere Microbiome Function and Plant Disease Suppression10.1128/mBio.01790-162150-7511https://doaj.org/article/b9f8ad852223458c8aee9bf607c783cb2016-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01790-16https://doaj.org/toc/2150-7511ABSTRACT Bacterial communities associated with plant roots play an important role in the suppression of soil-borne pathogens, and multispecies probiotic consortia may enhance disease suppression efficacy. Here we introduced defined Pseudomonas species consortia into naturally complex microbial communities and measured the importance of Pseudomonas community diversity for their survival and the suppression of the bacterial plant pathogen Ralstonia solanacearum in the tomato rhizosphere microbiome. The survival of introduced Pseudomonas consortia increased with increasing diversity. Further, high Pseudomonas diversity reduced pathogen density in the rhizosphere and decreased the disease incidence due to both intensified resource competition and interference with the pathogen. These results provide novel mechanistic insights into elevated pathogen suppression by diverse probiotic consortia in naturally diverse plant rhizospheres. Ecologically based community assembly rules could thus play a key role in engineering functionally reliable microbiome applications. IMPORTANCE The increasing demand for food supply requires more-efficient control of plant diseases. The use of probiotics, i.e., naturally occurring bacterial antagonists and competitors that suppress pathogens, has recently reemerged as a promising alternative to agrochemical use. It is, however, still unclear how many and which strains we should choose for constructing effective probiotic consortia. Here we present a general ecological framework for assembling effective probiotic communities based on in vitro characterization of community functioning. Specifically, we show that increasing the diversity of probiotic consortia enhances community survival in the naturally diverse rhizosphere microbiome, leading to increased pathogen suppression via intensified resource competition and interference with the pathogen. We propose that these ecological guidelines can be put to the test in microbiome engineering more widely in the future.Jie HuZhong WeiVille-Petri FrimanShao-hua GuXiao-fang WangNico EisenhauerTian-jie YangJing MaQi-rong ShenYang-chun XuAlexandre JoussetAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 7, Iss 6 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Jie Hu Zhong Wei Ville-Petri Friman Shao-hua Gu Xiao-fang Wang Nico Eisenhauer Tian-jie Yang Jing Ma Qi-rong Shen Yang-chun Xu Alexandre Jousset Probiotic Diversity Enhances Rhizosphere Microbiome Function and Plant Disease Suppression
description	ABSTRACT Bacterial communities associated with plant roots play an important role in the suppression of soil-borne pathogens, and multispecies probiotic consortia may enhance disease suppression efficacy. Here we introduced defined Pseudomonas species consortia into naturally complex microbial communities and measured the importance of Pseudomonas community diversity for their survival and the suppression of the bacterial plant pathogen Ralstonia solanacearum in the tomato rhizosphere microbiome. The survival of introduced Pseudomonas consortia increased with increasing diversity. Further, high Pseudomonas diversity reduced pathogen density in the rhizosphere and decreased the disease incidence due to both intensified resource competition and interference with the pathogen. These results provide novel mechanistic insights into elevated pathogen suppression by diverse probiotic consortia in naturally diverse plant rhizospheres. Ecologically based community assembly rules could thus play a key role in engineering functionally reliable microbiome applications. IMPORTANCE The increasing demand for food supply requires more-efficient control of plant diseases. The use of probiotics, i.e., naturally occurring bacterial antagonists and competitors that suppress pathogens, has recently reemerged as a promising alternative to agrochemical use. It is, however, still unclear how many and which strains we should choose for constructing effective probiotic consortia. Here we present a general ecological framework for assembling effective probiotic communities based on in vitro characterization of community functioning. Specifically, we show that increasing the diversity of probiotic consortia enhances community survival in the naturally diverse rhizosphere microbiome, leading to increased pathogen suppression via intensified resource competition and interference with the pathogen. We propose that these ecological guidelines can be put to the test in microbiome engineering more widely in the future.
format	article
author	Jie Hu Zhong Wei Ville-Petri Friman Shao-hua Gu Xiao-fang Wang Nico Eisenhauer Tian-jie Yang Jing Ma Qi-rong Shen Yang-chun Xu Alexandre Jousset
author_facet	Jie Hu Zhong Wei Ville-Petri Friman Shao-hua Gu Xiao-fang Wang Nico Eisenhauer Tian-jie Yang Jing Ma Qi-rong Shen Yang-chun Xu Alexandre Jousset
author_sort	Jie Hu
title	Probiotic Diversity Enhances Rhizosphere Microbiome Function and Plant Disease Suppression
title_short	Probiotic Diversity Enhances Rhizosphere Microbiome Function and Plant Disease Suppression
title_full	Probiotic Diversity Enhances Rhizosphere Microbiome Function and Plant Disease Suppression
title_fullStr	Probiotic Diversity Enhances Rhizosphere Microbiome Function and Plant Disease Suppression
title_full_unstemmed	Probiotic Diversity Enhances Rhizosphere Microbiome Function and Plant Disease Suppression
title_sort	probiotic diversity enhances rhizosphere microbiome function and plant disease suppression
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/b9f8ad852223458c8aee9bf607c783cb
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_version_	1718427481010077696

Probiotic Diversity Enhances Rhizosphere Microbiome Function and Plant Disease Suppression

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