Increased NIH 3T3 fibroblast functions on cell culture dishes which mimic the nanometer fibers of natural tissues

Increased NIH 3T3 fibroblast functions on cell culture dishes which mimic the nanometer fibers of natural tissues

Garima Bhardwaj,1 Thomas J Webster1,21Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi ArabiaAbstract: Traditional flat tissue cell culture dishes have consisted of polystyren...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Bhardwaj G, Webster TJ
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/b9f90901d8e941b5b7127749c835e4a9
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:b9f90901d8e941b5b7127749c835e4a9
record_format dspace
spelling oai:doaj.org-article:b9f90901d8e941b5b7127749c835e4a92021-12-02T04:23:13ZIncreased NIH 3T3 fibroblast functions on cell culture dishes which mimic the nanometer fibers of natural tissues1178-2013https://doaj.org/article/b9f90901d8e941b5b7127749c835e4a92015-08-01T00:00:00Zhttp://www.dovepress.com/increased-nih-3t3-fibroblast-functions-on-cell-culture-dishes-which-mi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Garima Bhardwaj,1 Thomas J Webster1,21Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi ArabiaAbstract: Traditional flat tissue cell culture dishes have consisted of polystyrene treated with plasma gases for growing, subculturing, and studying cell behavior in vitro. However, increasingly it has been observed that mimicking natural tissue properties (such as chemistry, three-dimensional structure, mechanical properties, etc) in vitro can lead to a better correlation of in vitro to in vivo cellular functions. The following studies compared traditional NIH 3T3 fibroblasts’ functions on XanoMatrix scaffolds to standard tissue culture polystyrene. Results found significantly greater fibroblast adhesion and proliferation on XanoMatrix cell culture dishes which mimic the nanoscale geometry of natural tissue fibers with true, tortuous fiber beds creating a robust, consistent, and versatile growth platform. In this manner, this study supports that cell culture dishes which mimic features of natural tissues should be continually studied for a wide range of applications in which mimicking natural cellular functions are important.Keywords: nanotechnology, cell culture, fibroblastsBhardwaj GWebster TJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 5293-5299 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Bhardwaj G
Webster TJ
Increased NIH 3T3 fibroblast functions on cell culture dishes which mimic the nanometer fibers of natural tissues
description Garima Bhardwaj,1 Thomas J Webster1,21Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi ArabiaAbstract: Traditional flat tissue cell culture dishes have consisted of polystyrene treated with plasma gases for growing, subculturing, and studying cell behavior in vitro. However, increasingly it has been observed that mimicking natural tissue properties (such as chemistry, three-dimensional structure, mechanical properties, etc) in vitro can lead to a better correlation of in vitro to in vivo cellular functions. The following studies compared traditional NIH 3T3 fibroblasts’ functions on XanoMatrix scaffolds to standard tissue culture polystyrene. Results found significantly greater fibroblast adhesion and proliferation on XanoMatrix cell culture dishes which mimic the nanoscale geometry of natural tissue fibers with true, tortuous fiber beds creating a robust, consistent, and versatile growth platform. In this manner, this study supports that cell culture dishes which mimic features of natural tissues should be continually studied for a wide range of applications in which mimicking natural cellular functions are important.Keywords: nanotechnology, cell culture, fibroblasts
format article
author Bhardwaj G
Webster TJ
author_facet Bhardwaj G
Webster TJ
author_sort Bhardwaj G
title Increased NIH 3T3 fibroblast functions on cell culture dishes which mimic the nanometer fibers of natural tissues
title_short Increased NIH 3T3 fibroblast functions on cell culture dishes which mimic the nanometer fibers of natural tissues
title_full Increased NIH 3T3 fibroblast functions on cell culture dishes which mimic the nanometer fibers of natural tissues
title_fullStr Increased NIH 3T3 fibroblast functions on cell culture dishes which mimic the nanometer fibers of natural tissues
title_full_unstemmed Increased NIH 3T3 fibroblast functions on cell culture dishes which mimic the nanometer fibers of natural tissues
title_sort increased nih 3t3 fibroblast functions on cell culture dishes which mimic the nanometer fibers of natural tissues
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/b9f90901d8e941b5b7127749c835e4a9
work_keys_str_mv AT bhardwajg increasednih3t3fibroblastfunctionsoncellculturedisheswhichmimicthenanometerfibersnbspofnaturaltissues
AT webstertj increasednih3t3fibroblastfunctionsoncellculturedisheswhichmimicthenanometerfibersnbspofnaturaltissues
_version_ 1718401269439135744

Ejemplares similares