Multiplex Immunoassays Utilizing Differential Affinity Using Aptamers Generated by MARAS

Abstract Disease diagnosis typically requires to determine concentration of multiple biomarkers in patient serums. Here, a novel method for multiplex immunoassays is proposed and the feasibility is demonstrated. The method utilizes the differential affinity between aptamers and multiple analytes for...

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Autores principales:	Ji-Ching Lai, Horng-Er Horng, Chin-Yih Hong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b9f909035f2440269ff0a11dec42ca8d
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spelling	oai:doaj.org-article:b9f909035f2440269ff0a11dec42ca8d2021-12-02T11:40:24ZMultiplex Immunoassays Utilizing Differential Affinity Using Aptamers Generated by MARAS10.1038/s41598-017-06950-12045-2322https://doaj.org/article/b9f909035f2440269ff0a11dec42ca8d2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06950-1https://doaj.org/toc/2045-2322Abstract Disease diagnosis typically requires to determine concentration of multiple biomarkers in patient serums. Here, a novel method for multiplex immunoassays is proposed and the feasibility is demonstrated. The method utilizes the differential affinity between aptamers and multiple analytes for multiplex immunoassays. During the selection, aptamers capable of binding to multiple analytes with different affinities are screened from a random oligonucleotide library using the MARAS procedure with different magnetic field conditions for different target analytes. During the detection, the same magnetic field conditions are applied to differentiate different target analytes in blind serums. The results show that the recovery rates of the spiked targets in BD buffer and blind serums are similar. Moreover, there is a minimal interference resulting from non-specific binding of molecules in serums other than the target molecules. Therefore, the use of differential affinities between aptamers and different analytes for multiplex immunoassays is proved to be feasible.Ji-Ching LaiHorng-Er HorngChin-Yih HongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ji-Ching Lai Horng-Er Horng Chin-Yih Hong Multiplex Immunoassays Utilizing Differential Affinity Using Aptamers Generated by MARAS
description	Abstract Disease diagnosis typically requires to determine concentration of multiple biomarkers in patient serums. Here, a novel method for multiplex immunoassays is proposed and the feasibility is demonstrated. The method utilizes the differential affinity between aptamers and multiple analytes for multiplex immunoassays. During the selection, aptamers capable of binding to multiple analytes with different affinities are screened from a random oligonucleotide library using the MARAS procedure with different magnetic field conditions for different target analytes. During the detection, the same magnetic field conditions are applied to differentiate different target analytes in blind serums. The results show that the recovery rates of the spiked targets in BD buffer and blind serums are similar. Moreover, there is a minimal interference resulting from non-specific binding of molecules in serums other than the target molecules. Therefore, the use of differential affinities between aptamers and different analytes for multiplex immunoassays is proved to be feasible.
format	article
author	Ji-Ching Lai Horng-Er Horng Chin-Yih Hong
author_facet	Ji-Ching Lai Horng-Er Horng Chin-Yih Hong
author_sort	Ji-Ching Lai
title	Multiplex Immunoassays Utilizing Differential Affinity Using Aptamers Generated by MARAS
title_short	Multiplex Immunoassays Utilizing Differential Affinity Using Aptamers Generated by MARAS
title_full	Multiplex Immunoassays Utilizing Differential Affinity Using Aptamers Generated by MARAS
title_fullStr	Multiplex Immunoassays Utilizing Differential Affinity Using Aptamers Generated by MARAS
title_full_unstemmed	Multiplex Immunoassays Utilizing Differential Affinity Using Aptamers Generated by MARAS
title_sort	multiplex immunoassays utilizing differential affinity using aptamers generated by maras
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b9f909035f2440269ff0a11dec42ca8d
work_keys_str_mv	AT jichinglai multipleximmunoassaysutilizingdifferentialaffinityusingaptamersgeneratedbymaras AT horngerhorng multipleximmunoassaysutilizingdifferentialaffinityusingaptamersgeneratedbymaras AT chinyihhong multipleximmunoassaysutilizingdifferentialaffinityusingaptamersgeneratedbymaras
_version_	1718395666400542720

Multiplex Immunoassays Utilizing Differential Affinity Using Aptamers Generated by MARAS

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