Effect of X-ray free-electron laser-induced shockwaves on haemoglobin microcrystals delivered in a liquid jet

Effect of X-ray free-electron laser-induced shockwaves on haemoglobin microcrystals delivered in a liquid jet

X-ray fee-electron lasers (XFELs) enable time-resolved crystallography experiments and the structure determination of proteins with little or no radiation damage. However currently it is unknown whether the designated 4.5 MHz maximum pulse rate for the European XFEL could lead to sample damage cause...

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Autores principales: Marie Luise Grünbein, Alexander Gorel, Lutz Foucar, Sergio Carbajo, William Colocho, Sasha Gilevich, Elisabeth Hartmann, Mario Hilpert, Mark Hunter, Marco Kloos, Jason E. Koglin, Thomas J. Lane, Jim Lewandowski, Alberto Lutman, Karol Nass, Gabriela Nass Kovacs, Christopher M. Roome, John Sheppard, Robert L. Shoeman, Miriam Stricker, Tim van Driel, Sharon Vetter, R. Bruce Doak, Sébastien Boutet, Andrew Aquila, Franz Josef Decker, Thomas R. M. Barends, Claudiu Andrei Stan, Ilme Schlichting
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Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/b9fa088215e4467aa2714fbb4e537fb1
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spelling oai:doaj.org-article:b9fa088215e4467aa2714fbb4e537fb12021-12-02T17:04:54ZEffect of X-ray free-electron laser-induced shockwaves on haemoglobin microcrystals delivered in a liquid jet10.1038/s41467-021-21819-82041-1723https://doaj.org/article/b9fa088215e4467aa2714fbb4e537fb12021-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21819-8https://doaj.org/toc/2041-1723X-ray fee-electron lasers (XFELs) enable time-resolved crystallography experiments and the structure determination of proteins with little or no radiation damage. However currently it is unknown whether the designated 4.5 MHz maximum pulse rate for the European XFEL could lead to sample damage caused by shock waves from preceding pulses. Here, the authors address this question by performing a X-ray pump X-ray probe experiment on haemoglobin microcrystals at the Stanford XFEL facility that mimics the 4.5 MHz data collection mode and observe structural changes and a drop in diffraction data quality of the crystals.Marie Luise GrünbeinAlexander GorelLutz FoucarSergio CarbajoWilliam ColochoSasha GilevichElisabeth HartmannMario HilpertMark HunterMarco KloosJason E. KoglinThomas J. LaneJim LewandowskiAlberto LutmanKarol NassGabriela Nass KovacsChristopher M. RoomeJohn SheppardRobert L. ShoemanMiriam StrickerTim van DrielSharon VetterR. Bruce DoakSébastien BoutetAndrew AquilaFranz Josef DeckerThomas R. M. BarendsClaudiu Andrei StanIlme SchlichtingNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Marie Luise Grünbein
Alexander Gorel
Lutz Foucar
Sergio Carbajo
William Colocho
Sasha Gilevich
Elisabeth Hartmann
Mario Hilpert
Mark Hunter
Marco Kloos
Jason E. Koglin
Thomas J. Lane
Jim Lewandowski
Alberto Lutman
Karol Nass
Gabriela Nass Kovacs
Christopher M. Roome
John Sheppard
Robert L. Shoeman
Miriam Stricker
Tim van Driel
Sharon Vetter
R. Bruce Doak
Sébastien Boutet
Andrew Aquila
Franz Josef Decker
Thomas R. M. Barends
Claudiu Andrei Stan
Ilme Schlichting
Effect of X-ray free-electron laser-induced shockwaves on haemoglobin microcrystals delivered in a liquid jet
description X-ray fee-electron lasers (XFELs) enable time-resolved crystallography experiments and the structure determination of proteins with little or no radiation damage. However currently it is unknown whether the designated 4.5 MHz maximum pulse rate for the European XFEL could lead to sample damage caused by shock waves from preceding pulses. Here, the authors address this question by performing a X-ray pump X-ray probe experiment on haemoglobin microcrystals at the Stanford XFEL facility that mimics the 4.5 MHz data collection mode and observe structural changes and a drop in diffraction data quality of the crystals.
format article
author Marie Luise Grünbein
Alexander Gorel
Lutz Foucar
Sergio Carbajo
William Colocho
Sasha Gilevich
Elisabeth Hartmann
Mario Hilpert
Mark Hunter
Marco Kloos
Jason E. Koglin
Thomas J. Lane
Jim Lewandowski
Alberto Lutman
Karol Nass
Gabriela Nass Kovacs
Christopher M. Roome
John Sheppard
Robert L. Shoeman
Miriam Stricker
Tim van Driel
Sharon Vetter
R. Bruce Doak
Sébastien Boutet
Andrew Aquila
Franz Josef Decker
Thomas R. M. Barends
Claudiu Andrei Stan
Ilme Schlichting
author_facet Marie Luise Grünbein
Alexander Gorel
Lutz Foucar
Sergio Carbajo
William Colocho
Sasha Gilevich
Elisabeth Hartmann
Mario Hilpert
Mark Hunter
Marco Kloos
Jason E. Koglin
Thomas J. Lane
Jim Lewandowski
Alberto Lutman
Karol Nass
Gabriela Nass Kovacs
Christopher M. Roome
John Sheppard
Robert L. Shoeman
Miriam Stricker
Tim van Driel
Sharon Vetter
R. Bruce Doak
Sébastien Boutet
Andrew Aquila
Franz Josef Decker
Thomas R. M. Barends
Claudiu Andrei Stan
Ilme Schlichting
author_sort Marie Luise Grünbein
title Effect of X-ray free-electron laser-induced shockwaves on haemoglobin microcrystals delivered in a liquid jet
title_short Effect of X-ray free-electron laser-induced shockwaves on haemoglobin microcrystals delivered in a liquid jet
title_full Effect of X-ray free-electron laser-induced shockwaves on haemoglobin microcrystals delivered in a liquid jet
title_fullStr Effect of X-ray free-electron laser-induced shockwaves on haemoglobin microcrystals delivered in a liquid jet
title_full_unstemmed Effect of X-ray free-electron laser-induced shockwaves on haemoglobin microcrystals delivered in a liquid jet
title_sort effect of x-ray free-electron laser-induced shockwaves on haemoglobin microcrystals delivered in a liquid jet
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/b9fa088215e4467aa2714fbb4e537fb1
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