ASSESSMENT OF SMOKE INDUCED GENOTOXICITY IN SPRAGUE DAWLEY RATS
Objective: To investigate the genotoxic effect of cigarette smoking in Sprague Dawley rats. Study Design: Randomized experimental study. Place and Duration of Study: Study was conducted at Army Medical College Rawalpindi, from Jan 2013 to Jul 2013. Material and Methods: Seventy healthy Spra...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Army Medical College Rawalpindi
2019
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/ba0c22d53811433982933f69cbee242e |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: | Objective: To investigate the genotoxic effect of cigarette smoking in Sprague Dawley rats.
Study Design: Randomized experimental study.
Place and Duration of Study: Study was conducted at Army Medical College Rawalpindi, from Jan 2013 to Jul 2013.
Material and Methods: Seventy healthy Sprague Dawley rats were randomly divided into two groups at National Institute of Health (NIH). Group-I rats were not exposed while Group-II rats were exposed to cigarette smoke for 3 months. Cytokinesis Block Micronucleus Assay (CBMN) was done to determine genotoxicity. Data were subjected to statistical analysis using independent sample t-test.
Results: The mean frequency of micronuclei in peripheral blood lymphocytes of control and smoker groups was 3.06 ± 0.639 and 6.77 ± 0.731 respectively. The difference was not statistically significant (p>0.05).
Conclusion: The mean micronuclei frequency in peripheral blood lymphocytes of the smoke exposed rats was higher than that of the control rats however the difference was not significant. |
|---|