Genome-Wide Analysis of the Fatty Acid Desaturase Gene Family Reveals the Key Role of PfFAD3 in α-Linolenic Acid Biosynthesis in Perilla Seeds

Perilla (Perilla frutescens), a traditional medicinal and oilseed crop in Asia, contains extremely high levels of polyunsaturated α-linolenic acid (ALA) (up to 60.9%) in its seeds. ALA biosynthesis is a multistep process catalyzed by fatty acid desaturases (FADs), but the FAD gene family in perilla...

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Autores principales:	Wu Duan, Yang Shi-Mei, Shang Zhi-Wei, Xu Jing, Zhao De-Gang, Wang Hong-Bin, Shen Qi
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	perilla α-linolenic acid (C18:3) fatty acid desaturases genome-wide analysis plant transformation Genetics QH426-470
Acceso en línea:	https://doaj.org/article/ba0c5f5be292482d9998e90a5b86d9cc
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spelling	oai:doaj.org-article:ba0c5f5be292482d9998e90a5b86d9cc2021-11-30T18:45:38ZGenome-Wide Analysis of the Fatty Acid Desaturase Gene Family Reveals the Key Role of PfFAD3 in α-Linolenic Acid Biosynthesis in Perilla Seeds1664-802110.3389/fgene.2021.735862https://doaj.org/article/ba0c5f5be292482d9998e90a5b86d9cc2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.735862/fullhttps://doaj.org/toc/1664-8021Perilla (Perilla frutescens), a traditional medicinal and oilseed crop in Asia, contains extremely high levels of polyunsaturated α-linolenic acid (ALA) (up to 60.9%) in its seeds. ALA biosynthesis is a multistep process catalyzed by fatty acid desaturases (FADs), but the FAD gene family in perilla has not been systematically characterized. Here, we identified 42 PfFADs in the perilla genome and classified them into five subfamilies. Subfamily members of PfFADs had similar exon/intron structures, conserved domain sequences, subcellular localizations, and cis-regulatory elements in their promoter regions. PfFADs also possessed various expression patterns. PfFAD3.1 was highly expressed in the middle stage of seed development, whereas PfFAD7/8.3 and PfFAD7/8.5 were highly expressed in leaf and later stages of seed development, respectively. Phylogenetic analysis revealed that the evolutionary features coincided with the functionalization of different subfamilies of PUFA desaturase. Heterologous overexpression of PfFAD3.1 in Arabidopsis thaliana seeds increased ALA content by 17.68%–37.03%. These findings provided insights into the characteristics and functions of PfFAD genes in perilla.Wu DuanWu DuanYang Shi-MeiShang Zhi-WeiXu JingZhao De-GangZhao De-GangWang Hong-BinShen QiFrontiers Media S.A.articleperillaα-linolenic acid (C18:3)fatty acid desaturasesgenome-wide analysisplant transformationGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	perilla α-linolenic acid (C18:3) fatty acid desaturases genome-wide analysis plant transformation Genetics QH426-470
spellingShingle	perilla α-linolenic acid (C18:3) fatty acid desaturases genome-wide analysis plant transformation Genetics QH426-470 Wu Duan Wu Duan Yang Shi-Mei Shang Zhi-Wei Xu Jing Zhao De-Gang Zhao De-Gang Wang Hong-Bin Shen Qi Genome-Wide Analysis of the Fatty Acid Desaturase Gene Family Reveals the Key Role of PfFAD3 in α-Linolenic Acid Biosynthesis in Perilla Seeds
description	Perilla (Perilla frutescens), a traditional medicinal and oilseed crop in Asia, contains extremely high levels of polyunsaturated α-linolenic acid (ALA) (up to 60.9%) in its seeds. ALA biosynthesis is a multistep process catalyzed by fatty acid desaturases (FADs), but the FAD gene family in perilla has not been systematically characterized. Here, we identified 42 PfFADs in the perilla genome and classified them into five subfamilies. Subfamily members of PfFADs had similar exon/intron structures, conserved domain sequences, subcellular localizations, and cis-regulatory elements in their promoter regions. PfFADs also possessed various expression patterns. PfFAD3.1 was highly expressed in the middle stage of seed development, whereas PfFAD7/8.3 and PfFAD7/8.5 were highly expressed in leaf and later stages of seed development, respectively. Phylogenetic analysis revealed that the evolutionary features coincided with the functionalization of different subfamilies of PUFA desaturase. Heterologous overexpression of PfFAD3.1 in Arabidopsis thaliana seeds increased ALA content by 17.68%–37.03%. These findings provided insights into the characteristics and functions of PfFAD genes in perilla.
format	article
author	Wu Duan Wu Duan Yang Shi-Mei Shang Zhi-Wei Xu Jing Zhao De-Gang Zhao De-Gang Wang Hong-Bin Shen Qi
author_facet	Wu Duan Wu Duan Yang Shi-Mei Shang Zhi-Wei Xu Jing Zhao De-Gang Zhao De-Gang Wang Hong-Bin Shen Qi
author_sort	Wu Duan
title	Genome-Wide Analysis of the Fatty Acid Desaturase Gene Family Reveals the Key Role of PfFAD3 in α-Linolenic Acid Biosynthesis in Perilla Seeds
title_short	Genome-Wide Analysis of the Fatty Acid Desaturase Gene Family Reveals the Key Role of PfFAD3 in α-Linolenic Acid Biosynthesis in Perilla Seeds
title_full	Genome-Wide Analysis of the Fatty Acid Desaturase Gene Family Reveals the Key Role of PfFAD3 in α-Linolenic Acid Biosynthesis in Perilla Seeds
title_fullStr	Genome-Wide Analysis of the Fatty Acid Desaturase Gene Family Reveals the Key Role of PfFAD3 in α-Linolenic Acid Biosynthesis in Perilla Seeds
title_full_unstemmed	Genome-Wide Analysis of the Fatty Acid Desaturase Gene Family Reveals the Key Role of PfFAD3 in α-Linolenic Acid Biosynthesis in Perilla Seeds
title_sort	genome-wide analysis of the fatty acid desaturase gene family reveals the key role of pffad3 in α-linolenic acid biosynthesis in perilla seeds
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ba0c5f5be292482d9998e90a5b86d9cc
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Genome-Wide Analysis of the Fatty Acid Desaturase Gene Family Reveals the Key Role of PfFAD3 in α-Linolenic Acid Biosynthesis in Perilla Seeds

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