Use of clodronate liposomes to deplete phagocytic immune cells in Drosophila melanogaster and Aedes aegypti

The innate immune system is the primary defense response to limit invading pathogens for all invertebrate species. In insects, immune cells are central to both cellular and humoral immune responses, however few genetic resources exist beyond Drosophila to study immune cell function. Therefore, the d...

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Autores principales:	Jyothsna Ramesh Kumar, Jessica P. Smith, Hyeogsun Kwon, Ryan C. Smith
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A.
Materias:	Phagocytosis Hemocytes immune cells phagocyte depletion Clodronate liposomes Aedes (Ae.) aegypti Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ba1271c6b58e49dfba33dc2255ec9ef0
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spelling	oai:doaj.org-article:ba1271c6b58e49dfba33dc2255ec9ef02021-12-03T11:59:53ZUse of clodronate liposomes to deplete phagocytic immune cells in Drosophila melanogaster and Aedes aegypti2296-634X10.3389/fcell.2021.627976https://doaj.org/article/ba1271c6b58e49dfba33dc2255ec9ef0https://www.frontiersin.org/articles/10.3389/fcell.2021.627976/fullhttps://doaj.org/toc/2296-634XThe innate immune system is the primary defense response to limit invading pathogens for all invertebrate species. In insects, immune cells are central to both cellular and humoral immune responses, however few genetic resources exist beyond Drosophila to study immune cell function. Therefore, the development of innovative tools that can be widely applied to a variety of insect systems is of importance to advance the study of insect immunity. Here, we have adapted the use of clodronate liposomes to deplete phagocytic immune cells in the mosquito, Aedes aegypti, and the vinegar fly, Drosophila melanogaster. Through a variety of microscopy and molecular techniques, we validate the depletion of phagocytic cell populations in both insect species and demonstrate the integral role of phagocytes in combating bacterial pathogens. Together, these data demonstrate the wide utility of clodronate liposomes in insect systems to advance the study of phagocyte function in insect innate immunity.Jyothsna Ramesh KumarJyothsna Ramesh KumarJessica P. SmithHyeogsun KwonRyan C. SmithFrontiers Media S.A.articlePhagocytosisHemocytesimmune cellsphagocyte depletionClodronate liposomesAedes (Ae.) aegyptiBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (0)
institution	DOAJ
collection	DOAJ
language	EN
topic	Phagocytosis Hemocytes immune cells phagocyte depletion Clodronate liposomes Aedes (Ae.) aegypti Biology (General) QH301-705.5
spellingShingle	Phagocytosis Hemocytes immune cells phagocyte depletion Clodronate liposomes Aedes (Ae.) aegypti Biology (General) QH301-705.5 Jyothsna Ramesh Kumar Jyothsna Ramesh Kumar Jessica P. Smith Hyeogsun Kwon Ryan C. Smith Use of clodronate liposomes to deplete phagocytic immune cells in Drosophila melanogaster and Aedes aegypti
description	The innate immune system is the primary defense response to limit invading pathogens for all invertebrate species. In insects, immune cells are central to both cellular and humoral immune responses, however few genetic resources exist beyond Drosophila to study immune cell function. Therefore, the development of innovative tools that can be widely applied to a variety of insect systems is of importance to advance the study of insect immunity. Here, we have adapted the use of clodronate liposomes to deplete phagocytic immune cells in the mosquito, Aedes aegypti, and the vinegar fly, Drosophila melanogaster. Through a variety of microscopy and molecular techniques, we validate the depletion of phagocytic cell populations in both insect species and demonstrate the integral role of phagocytes in combating bacterial pathogens. Together, these data demonstrate the wide utility of clodronate liposomes in insect systems to advance the study of phagocyte function in insect innate immunity.
format	article
author	Jyothsna Ramesh Kumar Jyothsna Ramesh Kumar Jessica P. Smith Hyeogsun Kwon Ryan C. Smith
author_facet	Jyothsna Ramesh Kumar Jyothsna Ramesh Kumar Jessica P. Smith Hyeogsun Kwon Ryan C. Smith
author_sort	Jyothsna Ramesh Kumar
title	Use of clodronate liposomes to deplete phagocytic immune cells in Drosophila melanogaster and Aedes aegypti
title_short	Use of clodronate liposomes to deplete phagocytic immune cells in Drosophila melanogaster and Aedes aegypti
title_full	Use of clodronate liposomes to deplete phagocytic immune cells in Drosophila melanogaster and Aedes aegypti
title_fullStr	Use of clodronate liposomes to deplete phagocytic immune cells in Drosophila melanogaster and Aedes aegypti
title_full_unstemmed	Use of clodronate liposomes to deplete phagocytic immune cells in Drosophila melanogaster and Aedes aegypti
title_sort	use of clodronate liposomes to deplete phagocytic immune cells in drosophila melanogaster and aedes aegypti
publisher	Frontiers Media S.A.
url	https://doaj.org/article/ba1271c6b58e49dfba33dc2255ec9ef0
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Use of clodronate liposomes to deplete phagocytic immune cells in Drosophila melanogaster and Aedes aegypti

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