Coenzyme Q10 Modulates Apoptotic Effects of Chronic Administration of Methadone on NMRI Mouse Hippocampus

Objective: Methadone is one of the widely used drug substances prescribed in treatment of opioid dependence and pain management; however, several studies have shown its neurotoxic effects on individuals and animal models. The purpose of this study was to assess neuroprotective effects of Coenzyme Q1...

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Autores principales:	Maryam Vaselbehagh, Mehdi Sadegh, Hadi Karami, Saied Babaie, Mohammad Hassan Sakhaie
Formato:	article
Lenguaje:	EN
Publicado:	Royan Institute (ACECR), Tehran 2021
Materias:	apoptosis bdnf coq10 hippocampus methadone Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ba193dec56234c7ba18f4a8b8248b667
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spelling	oai:doaj.org-article:ba193dec56234c7ba18f4a8b8248b6672021-11-07T07:21:44ZCoenzyme Q10 Modulates Apoptotic Effects of Chronic Administration of Methadone on NMRI Mouse Hippocampus10.22074/cellj.2021.73842228-58062228-5814https://doaj.org/article/ba193dec56234c7ba18f4a8b8248b6672021-10-01T00:00:00Zhttps://celljournal.org/journal/article/fulltext/coenzyme-q10-modulate-apoptotic-effects-of-chronic-methadone-on-nmri-mouse-hippocampus.pdfhttps://doaj.org/toc/2228-5806https://doaj.org/toc/2228-5814Objective: Methadone is one of the widely used drug substances prescribed in treatment of opioid dependence and pain management; however, several studies have shown its neurotoxic effects on individuals and animal models. The purpose of this study was to assess neuroprotective effects of Coenzyme Q10 (CoQ10) on neurotoxicity induced by methadone in hippocampus of adult NMRI male mice. Materials and Methods: In this experimental study, 48 adult NMRI male mice were randomly divided into 4 groups (n=12 in each) including Methadone, Methadone with sesame oil, Methadone with CoQ10 and saline. The injections of methadone, saline and sesame oil were performed intraperitoneally for 20 days. 24 hours after last injection, half of the animals in each group (n=6) were randomly assessed for evaluating of spatial memory by radial maze. Following behavioral study, animals were sacrificed, and their brains were removed to evaluate pyknotic cells through histological assessment. The remaining were used to study the expression of Arc, Bax, Bcl-2 and Bdnf genes. Results: Results of the present study showed that daily administration of methadone increased the number of pyknotic neurons in the CA1 hippocampus and altered the expression of Bax, Bdnf, Arc and Bcl-2. However, it did not alter spatial memory comparing to saline group. CoQ10 treatment significantly reduced the number of pyknotic cells and expression of Bax, Bdnf, Arc when compared to the vehicle group treated by sesame oil. However, the expression of Bcl-2 significantly increased as a result of CoQ10 treatment. Conclusion: CoQ10 reduced the neuronal damage caused by methadone in the hippocampus CA1.Maryam VaselbehaghMehdi SadeghHadi KaramiSaied BabaieMohammad Hassan SakhaieRoyan Institute (ACECR), Tehranarticleapoptosisbdnfcoq10hippocampusmethadoneMedicineRScienceQENCell Journal, Vol 23, Iss 5, Pp 538-543 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	apoptosis bdnf coq10 hippocampus methadone Medicine R Science Q
spellingShingle	apoptosis bdnf coq10 hippocampus methadone Medicine R Science Q Maryam Vaselbehagh Mehdi Sadegh Hadi Karami Saied Babaie Mohammad Hassan Sakhaie Coenzyme Q10 Modulates Apoptotic Effects of Chronic Administration of Methadone on NMRI Mouse Hippocampus
description	Objective: Methadone is one of the widely used drug substances prescribed in treatment of opioid dependence and pain management; however, several studies have shown its neurotoxic effects on individuals and animal models. The purpose of this study was to assess neuroprotective effects of Coenzyme Q10 (CoQ10) on neurotoxicity induced by methadone in hippocampus of adult NMRI male mice. Materials and Methods: In this experimental study, 48 adult NMRI male mice were randomly divided into 4 groups (n=12 in each) including Methadone, Methadone with sesame oil, Methadone with CoQ10 and saline. The injections of methadone, saline and sesame oil were performed intraperitoneally for 20 days. 24 hours after last injection, half of the animals in each group (n=6) were randomly assessed for evaluating of spatial memory by radial maze. Following behavioral study, animals were sacrificed, and their brains were removed to evaluate pyknotic cells through histological assessment. The remaining were used to study the expression of Arc, Bax, Bcl-2 and Bdnf genes. Results: Results of the present study showed that daily administration of methadone increased the number of pyknotic neurons in the CA1 hippocampus and altered the expression of Bax, Bdnf, Arc and Bcl-2. However, it did not alter spatial memory comparing to saline group. CoQ10 treatment significantly reduced the number of pyknotic cells and expression of Bax, Bdnf, Arc when compared to the vehicle group treated by sesame oil. However, the expression of Bcl-2 significantly increased as a result of CoQ10 treatment. Conclusion: CoQ10 reduced the neuronal damage caused by methadone in the hippocampus CA1.
format	article
author	Maryam Vaselbehagh Mehdi Sadegh Hadi Karami Saied Babaie Mohammad Hassan Sakhaie
author_facet	Maryam Vaselbehagh Mehdi Sadegh Hadi Karami Saied Babaie Mohammad Hassan Sakhaie
author_sort	Maryam Vaselbehagh
title	Coenzyme Q10 Modulates Apoptotic Effects of Chronic Administration of Methadone on NMRI Mouse Hippocampus
title_short	Coenzyme Q10 Modulates Apoptotic Effects of Chronic Administration of Methadone on NMRI Mouse Hippocampus
title_full	Coenzyme Q10 Modulates Apoptotic Effects of Chronic Administration of Methadone on NMRI Mouse Hippocampus
title_fullStr	Coenzyme Q10 Modulates Apoptotic Effects of Chronic Administration of Methadone on NMRI Mouse Hippocampus
title_full_unstemmed	Coenzyme Q10 Modulates Apoptotic Effects of Chronic Administration of Methadone on NMRI Mouse Hippocampus
title_sort	coenzyme q10 modulates apoptotic effects of chronic administration of methadone on nmri mouse hippocampus
publisher	Royan Institute (ACECR), Tehran
publishDate	2021
url	https://doaj.org/article/ba193dec56234c7ba18f4a8b8248b667
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Coenzyme Q10 Modulates Apoptotic Effects of Chronic Administration of Methadone on NMRI Mouse Hippocampus

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