Expression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms

Top et al. report the expression of a human blood-clotting factor IX-encoding cDNA in the moss Physcomitrium patens and demonstrate that in addition to the unspliced full-length transcript, the splicing machinery produced several different transcripts, and thus different protein isoforms, which the...

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Autores principales:	Oguz Top, Stella W. L. Milferstaedt, Nico van Gessel, Sebastian N. W. Hoernstein, Bugra Özdemir, Eva L. Decker, Ralf Reski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ba197c53d2034e1ba0d5a94102f55275
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spelling	oai:doaj.org-article:ba197c53d2034e1ba0d5a94102f552752021-12-02T15:08:37ZExpression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms10.1038/s42003-021-02486-32399-3642https://doaj.org/article/ba197c53d2034e1ba0d5a94102f552752021-08-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02486-3https://doaj.org/toc/2399-3642Top et al. report the expression of a human blood-clotting factor IX-encoding cDNA in the moss Physcomitrium patens and demonstrate that in addition to the unspliced full-length transcript, the splicing machinery produced several different transcripts, and thus different protein isoforms, which the authors designate as heterosplicing. The authors present a web application, physCO, to automatize the process of P. patens-specific codon optimization, a tool that can assist in maximized functional protein amounts for biotechnology.Oguz TopStella W. L. MilferstaedtNico van GesselSebastian N. W. HoernsteinBugra ÖzdemirEva L. DeckerRalf ReskiNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Oguz Top Stella W. L. Milferstaedt Nico van Gessel Sebastian N. W. Hoernstein Bugra Özdemir Eva L. Decker Ralf Reski Expression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms
description	Top et al. report the expression of a human blood-clotting factor IX-encoding cDNA in the moss Physcomitrium patens and demonstrate that in addition to the unspliced full-length transcript, the splicing machinery produced several different transcripts, and thus different protein isoforms, which the authors designate as heterosplicing. The authors present a web application, physCO, to automatize the process of P. patens-specific codon optimization, a tool that can assist in maximized functional protein amounts for biotechnology.
format	article
author	Oguz Top Stella W. L. Milferstaedt Nico van Gessel Sebastian N. W. Hoernstein Bugra Özdemir Eva L. Decker Ralf Reski
author_facet	Oguz Top Stella W. L. Milferstaedt Nico van Gessel Sebastian N. W. Hoernstein Bugra Özdemir Eva L. Decker Ralf Reski
author_sort	Oguz Top
title	Expression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms
title_short	Expression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms
title_full	Expression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms
title_fullStr	Expression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms
title_full_unstemmed	Expression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms
title_sort	expression of a human cdna in moss results in spliced mrnas and fragmentary protein isoforms
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ba197c53d2034e1ba0d5a94102f55275
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Expression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms

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