Composition and geographic variation of the bacterial microbiota associated with the coelomic fluid of the sea urchin Paracentrotus lividus
Abstract In the present work, culture-based and culture-independent investigations were performed to determine the microbiota structure of the coelomic fluid of Mediterranean sea urchin Paracentrotus lividus individuals collected from two distinct geographical sites neighboring a high-density popula...
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2020
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oai:doaj.org-article:ba2361b7fd8e48f3ab4c8dbfe7ceff882021-12-02T12:33:14ZComposition and geographic variation of the bacterial microbiota associated with the coelomic fluid of the sea urchin Paracentrotus lividus10.1038/s41598-020-78534-52045-2322https://doaj.org/article/ba2361b7fd8e48f3ab4c8dbfe7ceff882020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78534-5https://doaj.org/toc/2045-2322Abstract In the present work, culture-based and culture-independent investigations were performed to determine the microbiota structure of the coelomic fluid of Mediterranean sea urchin Paracentrotus lividus individuals collected from two distinct geographical sites neighboring a high-density population bay and a nature reserve, respectively. Next Generation Sequencing analysis of 16S rRNA gene (rDNA) showed that members of the Proteobacteria, Bacteroidetes and Fusobacteria phyla, which have been previously reported to be commonly retrieved from marine invertebrates, dominate the overall population of microorganisms colonizing this liquid tissue, with minority bacterial genera exhibiting remarkable differences among individuals. Our results showed that there is a correlation between microbiota structure and geographical location of the echinoderm collection site, highlighting over-representation of metagenomic functions related to amino acid and bioactive peptides metabolism in specimens inhabiting the nature reserve. Finally, we also described the developmental delay and aberrations exhibited by sea urchin embryos exposed to distinct bacterial isolates, and showed that these defects rely upon hydrophilic compound(s) synthesized by the bacterial strains assayed. Altogether, our findings lay the groundwork to decipher the relationships of bacteria with sea urchins in their aquatic environment, also providing an additional layer of information to understand the biological roles of the coelomic fluid.Teresa FaddettaFrancesco ArdizzoneFrancesca FaillaciChiara ReinaEmilia PalazzottoFrancesco StratiCarlotta De FilippoGiovanni SpinelliAnna Maria PugliaGiuseppe GalloVincenzo CavalieriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) |
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Medicine R Science Q Teresa Faddetta Francesco Ardizzone Francesca Faillaci Chiara Reina Emilia Palazzotto Francesco Strati Carlotta De Filippo Giovanni Spinelli Anna Maria Puglia Giuseppe Gallo Vincenzo Cavalieri Composition and geographic variation of the bacterial microbiota associated with the coelomic fluid of the sea urchin Paracentrotus lividus |
| description |
Abstract In the present work, culture-based and culture-independent investigations were performed to determine the microbiota structure of the coelomic fluid of Mediterranean sea urchin Paracentrotus lividus individuals collected from two distinct geographical sites neighboring a high-density population bay and a nature reserve, respectively. Next Generation Sequencing analysis of 16S rRNA gene (rDNA) showed that members of the Proteobacteria, Bacteroidetes and Fusobacteria phyla, which have been previously reported to be commonly retrieved from marine invertebrates, dominate the overall population of microorganisms colonizing this liquid tissue, with minority bacterial genera exhibiting remarkable differences among individuals. Our results showed that there is a correlation between microbiota structure and geographical location of the echinoderm collection site, highlighting over-representation of metagenomic functions related to amino acid and bioactive peptides metabolism in specimens inhabiting the nature reserve. Finally, we also described the developmental delay and aberrations exhibited by sea urchin embryos exposed to distinct bacterial isolates, and showed that these defects rely upon hydrophilic compound(s) synthesized by the bacterial strains assayed. Altogether, our findings lay the groundwork to decipher the relationships of bacteria with sea urchins in their aquatic environment, also providing an additional layer of information to understand the biological roles of the coelomic fluid. |
| format |
article |
| author |
Teresa Faddetta Francesco Ardizzone Francesca Faillaci Chiara Reina Emilia Palazzotto Francesco Strati Carlotta De Filippo Giovanni Spinelli Anna Maria Puglia Giuseppe Gallo Vincenzo Cavalieri |
| author_facet |
Teresa Faddetta Francesco Ardizzone Francesca Faillaci Chiara Reina Emilia Palazzotto Francesco Strati Carlotta De Filippo Giovanni Spinelli Anna Maria Puglia Giuseppe Gallo Vincenzo Cavalieri |
| author_sort |
Teresa Faddetta |
| title |
Composition and geographic variation of the bacterial microbiota associated with the coelomic fluid of the sea urchin Paracentrotus lividus |
| title_short |
Composition and geographic variation of the bacterial microbiota associated with the coelomic fluid of the sea urchin Paracentrotus lividus |
| title_full |
Composition and geographic variation of the bacterial microbiota associated with the coelomic fluid of the sea urchin Paracentrotus lividus |
| title_fullStr |
Composition and geographic variation of the bacterial microbiota associated with the coelomic fluid of the sea urchin Paracentrotus lividus |
| title_full_unstemmed |
Composition and geographic variation of the bacterial microbiota associated with the coelomic fluid of the sea urchin Paracentrotus lividus |
| title_sort |
composition and geographic variation of the bacterial microbiota associated with the coelomic fluid of the sea urchin paracentrotus lividus |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/ba2361b7fd8e48f3ab4c8dbfe7ceff88 |
| work_keys_str_mv |
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