Empirical equation for the correction of fluorescence quenching of proteinaceous substance by Suwannee river natural organic matter

Fluorescence quenching of proteinaceous substances by natural organic matter is a well-known phenomenon, but there are no known methods for correcting it. The main objective of this research was to develop an empirical equation to correct the fluorescence quenching of different concentrations of bov...

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Autores principales:	Kornravee Saipetch, Rajendra Khanal, Chihiro Yoshimura
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	bovine serum albumin dissolved organic matter fluorescence titration parafac protein-humic substance complex excitation emission matrix Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/ba2c39a8421d4460a72ccde72e5d0295
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spelling	oai:doaj.org-article:ba2c39a8421d4460a72ccde72e5d02952021-11-05T21:09:39ZEmpirical equation for the correction of fluorescence quenching of proteinaceous substance by Suwannee river natural organic matter1751-231X10.2166/wpt.2021.001https://doaj.org/article/ba2c39a8421d4460a72ccde72e5d02952021-04-01T00:00:00Zhttp://wpt.iwaponline.com/content/16/2/344https://doaj.org/toc/1751-231XFluorescence quenching of proteinaceous substances by natural organic matter is a well-known phenomenon, but there are no known methods for correcting it. The main objective of this research was to develop an empirical equation to correct the fluorescence quenching of different concentrations of bovine serum albumin (BSA – 0.15, 0.25, 0.5, 0.75, 1, 1.25 μmol/L (μM)) by Suwannee river natural organic matter (SWNOM - 0,2,4,6,8,10 mg-C/L) using the fluorescence titration method. The excitation emission matrix (EEM) data were analyzed by parallel factor analysis with inner filter effect removal. With increasing SWNOM concentration, BSA peak intensity quenching was in the range 29–85%, with a linear relationship for increment of either BSA or SWNOM concentration. A higher ratio of SWNOM to BSA resulted in greater BSA peak intensity quenching. The unquenched BSA peak (BSA (RU)) is given by the empirical equation. BSA (RU) = {2.052 × peak C (RU) + 2.522} × quenched peak T (RU)0.624 The calculated unquenched BSA peak intensities using the empirical equation agreed well with the actual unquenched peak values (R2 = 0.98, mean absolute error = 0.33 RU). The equation is expected to help in rapid estimation of the quenching effect of SWNOM on BSA. Highlights Empirical equation to correct the fluorescence quenching of bovine serum albumin by Suwannee river natural organic matter is discussed.; The EEM data was analyzed by parallel factor analysis with inner filter effect removal.; With an increase in SWNOM concentration, quenching of BSA peak intensity was in the range 29–85%.; Higher ratio of SWNOM to BSA resulted in higher quenching of the peak intensity of BSA.;Kornravee SaipetchRajendra KhanalChihiro YoshimuraIWA Publishingarticlebovine serum albumindissolved organic matterfluorescence titrationparafacprotein-humic substance complexexcitation emission matrixEnvironmental technology. Sanitary engineeringTD1-1066ENWater Practice and Technology, Vol 16, Iss 2, Pp 344-350 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bovine serum albumin dissolved organic matter fluorescence titration parafac protein-humic substance complex excitation emission matrix Environmental technology. Sanitary engineering TD1-1066
spellingShingle	bovine serum albumin dissolved organic matter fluorescence titration parafac protein-humic substance complex excitation emission matrix Environmental technology. Sanitary engineering TD1-1066 Kornravee Saipetch Rajendra Khanal Chihiro Yoshimura Empirical equation for the correction of fluorescence quenching of proteinaceous substance by Suwannee river natural organic matter
description	Fluorescence quenching of proteinaceous substances by natural organic matter is a well-known phenomenon, but there are no known methods for correcting it. The main objective of this research was to develop an empirical equation to correct the fluorescence quenching of different concentrations of bovine serum albumin (BSA – 0.15, 0.25, 0.5, 0.75, 1, 1.25 μmol/L (μM)) by Suwannee river natural organic matter (SWNOM - 0,2,4,6,8,10 mg-C/L) using the fluorescence titration method. The excitation emission matrix (EEM) data were analyzed by parallel factor analysis with inner filter effect removal. With increasing SWNOM concentration, BSA peak intensity quenching was in the range 29–85%, with a linear relationship for increment of either BSA or SWNOM concentration. A higher ratio of SWNOM to BSA resulted in greater BSA peak intensity quenching. The unquenched BSA peak (BSA (RU)) is given by the empirical equation. BSA (RU) = {2.052 × peak C (RU) + 2.522} × quenched peak T (RU)0.624 The calculated unquenched BSA peak intensities using the empirical equation agreed well with the actual unquenched peak values (R2 = 0.98, mean absolute error = 0.33 RU). The equation is expected to help in rapid estimation of the quenching effect of SWNOM on BSA. Highlights Empirical equation to correct the fluorescence quenching of bovine serum albumin by Suwannee river natural organic matter is discussed.; The EEM data was analyzed by parallel factor analysis with inner filter effect removal.; With an increase in SWNOM concentration, quenching of BSA peak intensity was in the range 29–85%.; Higher ratio of SWNOM to BSA resulted in higher quenching of the peak intensity of BSA.;
format	article
author	Kornravee Saipetch Rajendra Khanal Chihiro Yoshimura
author_facet	Kornravee Saipetch Rajendra Khanal Chihiro Yoshimura
author_sort	Kornravee Saipetch
title	Empirical equation for the correction of fluorescence quenching of proteinaceous substance by Suwannee river natural organic matter
title_short	Empirical equation for the correction of fluorescence quenching of proteinaceous substance by Suwannee river natural organic matter
title_full	Empirical equation for the correction of fluorescence quenching of proteinaceous substance by Suwannee river natural organic matter
title_fullStr	Empirical equation for the correction of fluorescence quenching of proteinaceous substance by Suwannee river natural organic matter
title_full_unstemmed	Empirical equation for the correction of fluorescence quenching of proteinaceous substance by Suwannee river natural organic matter
title_sort	empirical equation for the correction of fluorescence quenching of proteinaceous substance by suwannee river natural organic matter
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/ba2c39a8421d4460a72ccde72e5d0295
work_keys_str_mv	AT kornraveesaipetch empiricalequationforthecorrectionoffluorescencequenchingofproteinaceoussubstancebysuwanneerivernaturalorganicmatter AT rajendrakhanal empiricalequationforthecorrectionoffluorescencequenchingofproteinaceoussubstancebysuwanneerivernaturalorganicmatter AT chihiroyoshimura empiricalequationforthecorrectionoffluorescencequenchingofproteinaceoussubstancebysuwanneerivernaturalorganicmatter
_version_	1718444061190258688

Empirical equation for the correction of fluorescence quenching of proteinaceous substance by Suwannee river natural organic matter

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