Silencing Long Non-coding RNA Kcnq1ot1 Limits Acute Kidney Injury by Promoting miR-204-5p and Blocking the Activation of NLRP3 Inflammasome

Acute kidney injury (AKI) is a critical clinical disease characterized by an acute decrease in renal function. Long non-coding RNAs (LncRNAs) are important in AKI. This study aimed to explore the mechanism of lncRNA Kcnq1ot1 in AKI by sponging microRNA (miR)-204-5p as a competitive endogenous RNA (c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	JunTao Wang, Peng Jiao, XiaoYing Wei, Yun Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	lncRNA Kcnq1ot1 competitive endogenous RNA miR-204-5p NLRP3 inflammasome lipopolysaccharide Physiology QP1-981
Acceso en línea:	https://doaj.org/article/ba3497e3b9744027b39b9f15741a61c7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ba3497e3b9744027b39b9f15741a61c7
record_format	dspace
spelling	oai:doaj.org-article:ba3497e3b9744027b39b9f15741a61c72021-11-11T09:44:12ZSilencing Long Non-coding RNA Kcnq1ot1 Limits Acute Kidney Injury by Promoting miR-204-5p and Blocking the Activation of NLRP3 Inflammasome1664-042X10.3389/fphys.2021.721524https://doaj.org/article/ba3497e3b9744027b39b9f15741a61c72021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.721524/fullhttps://doaj.org/toc/1664-042XAcute kidney injury (AKI) is a critical clinical disease characterized by an acute decrease in renal function. Long non-coding RNAs (LncRNAs) are important in AKI. This study aimed to explore the mechanism of lncRNA Kcnq1ot1 in AKI by sponging microRNA (miR)-204-5p as a competitive endogenous RNA (ceRNA). AKI mouse model and hypoxia/reoxygenation (H/R) model of human kidney (HK) cells were established. Kcnq1ot1 expression, cell proliferation, and apoptosis were measured. Binding relations among Kcnq1ot1, miR-204-5p, and NLRP3 were verified. Pathological changes and cell apoptosis were detected. The results showed that Kcnq1ot1 was highly expressed in the AKI model in vivo and in vitro. Kcnq1ot1 knockdown promoted cell proliferation and prevented apoptosis and inflammation. Furthermore, Kcnq1ot1 inhibited miR-204-5p expression by competitively binding to miR-204-5p in HK-2 cells. miR-204-5p targeted NLRP3 and NLRP3 overexpression averted the inhibiting effect of miR-204-5p on apoptosis and inflammation in HK-2 cells in vitro. Kcnq1ot1 knockdown in vivo promoted miR-204-5p expression, inhibited NLRP3 inflammasome activation, reduced levels of SCr, BUN, and KIM-1, and thus alleviated AKI and reduced apoptosis. In summary, silencing lncRNA Kcnq1ot1 inhibited AKI by promoting miR-204-5p and inhibiting NLRP3 inflammasome activation.JunTao WangPeng JiaoXiaoYing WeiYun ZhouFrontiers Media S.A.articlelncRNA Kcnq1ot1competitive endogenous RNAmiR-204-5pNLRP3inflammasomelipopolysaccharidePhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lncRNA Kcnq1ot1 competitive endogenous RNA miR-204-5p NLRP3 inflammasome lipopolysaccharide Physiology QP1-981
spellingShingle	lncRNA Kcnq1ot1 competitive endogenous RNA miR-204-5p NLRP3 inflammasome lipopolysaccharide Physiology QP1-981 JunTao Wang Peng Jiao XiaoYing Wei Yun Zhou Silencing Long Non-coding RNA Kcnq1ot1 Limits Acute Kidney Injury by Promoting miR-204-5p and Blocking the Activation of NLRP3 Inflammasome
description	Acute kidney injury (AKI) is a critical clinical disease characterized by an acute decrease in renal function. Long non-coding RNAs (LncRNAs) are important in AKI. This study aimed to explore the mechanism of lncRNA Kcnq1ot1 in AKI by sponging microRNA (miR)-204-5p as a competitive endogenous RNA (ceRNA). AKI mouse model and hypoxia/reoxygenation (H/R) model of human kidney (HK) cells were established. Kcnq1ot1 expression, cell proliferation, and apoptosis were measured. Binding relations among Kcnq1ot1, miR-204-5p, and NLRP3 were verified. Pathological changes and cell apoptosis were detected. The results showed that Kcnq1ot1 was highly expressed in the AKI model in vivo and in vitro. Kcnq1ot1 knockdown promoted cell proliferation and prevented apoptosis and inflammation. Furthermore, Kcnq1ot1 inhibited miR-204-5p expression by competitively binding to miR-204-5p in HK-2 cells. miR-204-5p targeted NLRP3 and NLRP3 overexpression averted the inhibiting effect of miR-204-5p on apoptosis and inflammation in HK-2 cells in vitro. Kcnq1ot1 knockdown in vivo promoted miR-204-5p expression, inhibited NLRP3 inflammasome activation, reduced levels of SCr, BUN, and KIM-1, and thus alleviated AKI and reduced apoptosis. In summary, silencing lncRNA Kcnq1ot1 inhibited AKI by promoting miR-204-5p and inhibiting NLRP3 inflammasome activation.
format	article
author	JunTao Wang Peng Jiao XiaoYing Wei Yun Zhou
author_facet	JunTao Wang Peng Jiao XiaoYing Wei Yun Zhou
author_sort	JunTao Wang
title	Silencing Long Non-coding RNA Kcnq1ot1 Limits Acute Kidney Injury by Promoting miR-204-5p and Blocking the Activation of NLRP3 Inflammasome
title_short	Silencing Long Non-coding RNA Kcnq1ot1 Limits Acute Kidney Injury by Promoting miR-204-5p and Blocking the Activation of NLRP3 Inflammasome
title_full	Silencing Long Non-coding RNA Kcnq1ot1 Limits Acute Kidney Injury by Promoting miR-204-5p and Blocking the Activation of NLRP3 Inflammasome
title_fullStr	Silencing Long Non-coding RNA Kcnq1ot1 Limits Acute Kidney Injury by Promoting miR-204-5p and Blocking the Activation of NLRP3 Inflammasome
title_full_unstemmed	Silencing Long Non-coding RNA Kcnq1ot1 Limits Acute Kidney Injury by Promoting miR-204-5p and Blocking the Activation of NLRP3 Inflammasome
title_sort	silencing long non-coding rna kcnq1ot1 limits acute kidney injury by promoting mir-204-5p and blocking the activation of nlrp3 inflammasome
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ba3497e3b9744027b39b9f15741a61c7
work_keys_str_mv	AT juntaowang silencinglongnoncodingrnakcnq1ot1limitsacutekidneyinjurybypromotingmir2045pandblockingtheactivationofnlrp3inflammasome AT pengjiao silencinglongnoncodingrnakcnq1ot1limitsacutekidneyinjurybypromotingmir2045pandblockingtheactivationofnlrp3inflammasome AT xiaoyingwei silencinglongnoncodingrnakcnq1ot1limitsacutekidneyinjurybypromotingmir2045pandblockingtheactivationofnlrp3inflammasome AT yunzhou silencinglongnoncodingrnakcnq1ot1limitsacutekidneyinjurybypromotingmir2045pandblockingtheactivationofnlrp3inflammasome
_version_	1718439253628682240

Silencing Long Non-coding RNA Kcnq1ot1 Limits Acute Kidney Injury by Promoting miR-204-5p and Blocking the Activation of NLRP3 Inflammasome

Ejemplares similares