Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement.

To create novel variants for morphological, physiological, and biotic stress tolerance traits, induced mutations were created using Ethyl Methane Sulphonate (EMS) in the background of Samba Mahsuri (BPT 5204), a popular and mega rice variety of India. A population derived from 10, 500 M1 plants and...

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Autores principales:	Gopi Potupureddi, Vishalakshi Balija, Suneel Ballichatla, Gokulan C G, Komal Awalellu, Swathi Lekkala, Karteek Jallipalli, Gayathri M G, Ershad Mohammad, Milton M, Srikanth Arutla, Rajender Burka, Laha Gouri Shankar, Padmakumari Ayyangari Phani, SubbaRao Lella Venkata, Sundaram Raman Meenakshi, Viraktamath B C, Ravindra Babu Vemuri, Kranthi Brahma, Raju Madnala, Hitendra Kumar Patel, Ramesh Venkata Sonti, Maganti Sheshu Madhav
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ba35861a81994016b293e320c25b8c55
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spelling	oai:doaj.org-article:ba35861a81994016b293e320c25b8c552021-12-02T20:16:42ZMutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement.1932-620310.1371/journal.pone.0258816https://doaj.org/article/ba35861a81994016b293e320c25b8c552021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258816https://doaj.org/toc/1932-6203To create novel variants for morphological, physiological, and biotic stress tolerance traits, induced mutations were created using Ethyl Methane Sulphonate (EMS) in the background of Samba Mahsuri (BPT 5204), a popular and mega rice variety of India. A population derived from 10, 500 M1 plants and their descendants were phenotyped for a wide range of traits leading to the identification of 124 mutants having variations in key agro-morphological traits, and 106 mutants exhibiting variation for physiological traits. Higher yield is the ultimate goal of crop improvement and we identified 574 mutants having higher yield compared to wild type by having better yield attributing traits. Further, a total of 50 mutants showed better panicle exertion phenotypes as compared to Samba Mahsuri leading to enhancement of yield. Upon rigorous screening for three major biotic stresses, 8 mutants showed enhanced tolerance for yellow stem borer (YSB), and 13 different mutants each showed enhanced tolerance for sheath blight (ShB) and bacterial leaf blight (BLB), respectively. In addition, screening at multiple locations that have diverse field isolates identified 3, 3, and 5 lines for tolerance to ShB, YSB and BLB, respectively. On the whole, 1231 desired mutant lines identified at M2 were forwarded to an advanced generation (M5). PCR based allele mining indicated that the BLB tolerant mutants have a different allele than the reported alleles for well-known genes affecting bacterial blight resistance. Whole genome re-sequencing revealed substantial variation in comparison to Samba Mahsuri. The lines showing enhanced tolerance to important biotic stresses (YSB, ShB and BLB) as well as several economically important traits are unique genetic resources which can be utilized for the identification of novel genes/alleles for different traits. The lines which have better agronomic features can be used as pre-breeding lines. The entire mutant population is maintained as a national resource for genetic improvement of the rice crop.Gopi PotupureddiVishalakshi BalijaSuneel BallichatlaGokulan C GKomal AwalelluSwathi LekkalaKarteek JallipalliGayathri M GErshad MohammadMilton MSrikanth ArutlaRajender BurkaLaha Gouri ShankarPadmakumari Ayyangari PhaniSubbaRao Lella VenkataSundaram Raman MeenakshiViraktamath B CRavindra Babu VemuriKranthi BrahmaRaju MadnalaHitendra Kumar PatelRamesh Venkata SontiMaganti Sheshu MadhavPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258816 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gopi Potupureddi Vishalakshi Balija Suneel Ballichatla Gokulan C G Komal Awalellu Swathi Lekkala Karteek Jallipalli Gayathri M G Ershad Mohammad Milton M Srikanth Arutla Rajender Burka Laha Gouri Shankar Padmakumari Ayyangari Phani SubbaRao Lella Venkata Sundaram Raman Meenakshi Viraktamath B C Ravindra Babu Vemuri Kranthi Brahma Raju Madnala Hitendra Kumar Patel Ramesh Venkata Sonti Maganti Sheshu Madhav Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement.
description	To create novel variants for morphological, physiological, and biotic stress tolerance traits, induced mutations were created using Ethyl Methane Sulphonate (EMS) in the background of Samba Mahsuri (BPT 5204), a popular and mega rice variety of India. A population derived from 10, 500 M1 plants and their descendants were phenotyped for a wide range of traits leading to the identification of 124 mutants having variations in key agro-morphological traits, and 106 mutants exhibiting variation for physiological traits. Higher yield is the ultimate goal of crop improvement and we identified 574 mutants having higher yield compared to wild type by having better yield attributing traits. Further, a total of 50 mutants showed better panicle exertion phenotypes as compared to Samba Mahsuri leading to enhancement of yield. Upon rigorous screening for three major biotic stresses, 8 mutants showed enhanced tolerance for yellow stem borer (YSB), and 13 different mutants each showed enhanced tolerance for sheath blight (ShB) and bacterial leaf blight (BLB), respectively. In addition, screening at multiple locations that have diverse field isolates identified 3, 3, and 5 lines for tolerance to ShB, YSB and BLB, respectively. On the whole, 1231 desired mutant lines identified at M2 were forwarded to an advanced generation (M5). PCR based allele mining indicated that the BLB tolerant mutants have a different allele than the reported alleles for well-known genes affecting bacterial blight resistance. Whole genome re-sequencing revealed substantial variation in comparison to Samba Mahsuri. The lines showing enhanced tolerance to important biotic stresses (YSB, ShB and BLB) as well as several economically important traits are unique genetic resources which can be utilized for the identification of novel genes/alleles for different traits. The lines which have better agronomic features can be used as pre-breeding lines. The entire mutant population is maintained as a national resource for genetic improvement of the rice crop.
format	article
author	Gopi Potupureddi Vishalakshi Balija Suneel Ballichatla Gokulan C G Komal Awalellu Swathi Lekkala Karteek Jallipalli Gayathri M G Ershad Mohammad Milton M Srikanth Arutla Rajender Burka Laha Gouri Shankar Padmakumari Ayyangari Phani SubbaRao Lella Venkata Sundaram Raman Meenakshi Viraktamath B C Ravindra Babu Vemuri Kranthi Brahma Raju Madnala Hitendra Kumar Patel Ramesh Venkata Sonti Maganti Sheshu Madhav
author_facet	Gopi Potupureddi Vishalakshi Balija Suneel Ballichatla Gokulan C G Komal Awalellu Swathi Lekkala Karteek Jallipalli Gayathri M G Ershad Mohammad Milton M Srikanth Arutla Rajender Burka Laha Gouri Shankar Padmakumari Ayyangari Phani SubbaRao Lella Venkata Sundaram Raman Meenakshi Viraktamath B C Ravindra Babu Vemuri Kranthi Brahma Raju Madnala Hitendra Kumar Patel Ramesh Venkata Sonti Maganti Sheshu Madhav
author_sort	Gopi Potupureddi
title	Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement.
title_short	Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement.
title_full	Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement.
title_fullStr	Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement.
title_full_unstemmed	Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement.
title_sort	mutation resource of samba mahsuri revealed the presence of high extent of variations among key traits for rice improvement.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/ba35861a81994016b293e320c25b8c55
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Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement.

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