Modulation of human immune responses by bovine interleukin-10.

Cytokines can be functionally active across species barriers. Bovine IL-10 has an amino acid sequence identity with human IL-10 of 76.8%. Therefore, the aim of this study was to evaluate whether bovine IL-10 has immunomodulatory activities on human monocytes and dendritic cells. Peripheral blood mon...

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Autores principales:	Gerco den Hartog, Huub F J Savelkoul, Ruud Schoemaker, Edwin Tijhaar, Adrie H Westphal, Talitha de Ruiter, Elise van de Weg-Schrijver, R J Joost van Neerven
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ba36882c9a20402f9b561788c72c2b7c
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spelling	oai:doaj.org-article:ba36882c9a20402f9b561788c72c2b7c2021-11-18T06:56:47ZModulation of human immune responses by bovine interleukin-10.1932-620310.1371/journal.pone.0018188https://doaj.org/article/ba36882c9a20402f9b561788c72c2b7c2011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21464967/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Cytokines can be functionally active across species barriers. Bovine IL-10 has an amino acid sequence identity with human IL-10 of 76.8%. Therefore, the aim of this study was to evaluate whether bovine IL-10 has immunomodulatory activities on human monocytes and dendritic cells. Peripheral blood monocytes were isolated from healthy donors, and used directly or allowed to differentiate to dendritic cells under the influence of IL-4 and GM-CSF. Recombinant bovine IL-10 inhibited TLR induced activation of monocytes, and dose-dependently inhibited LPS-induced activation of monocyte-derived DCs comparable to human IL-10. By using blocking antibodies to either bovine IL-10 or the human IL-10 receptor it was demonstrated that inhibition of monocyte activation by bovine IL-10 was dependent on binding of bovine IL-10 to the human IL-10R. These data demonstrate that bovine IL-10 potently inhibits the activation of human myeloid cells in response to TLR activation. Bovine IL-10 present in dairy products may thus potentially contribute to the prevention of necrotizing enterocolitis and allergy, enhance mucosal tolerance induction and decrease intestinal inflammation and may therefore be applicable in infant foods and in immunomodulatory diets.Gerco den HartogHuub F J SavelkoulRuud SchoemakerEdwin TijhaarAdrie H WestphalTalitha de RuiterElise van de Weg-SchrijverR J Joost van NeervenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e18188 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gerco den Hartog Huub F J Savelkoul Ruud Schoemaker Edwin Tijhaar Adrie H Westphal Talitha de Ruiter Elise van de Weg-Schrijver R J Joost van Neerven Modulation of human immune responses by bovine interleukin-10.
description	Cytokines can be functionally active across species barriers. Bovine IL-10 has an amino acid sequence identity with human IL-10 of 76.8%. Therefore, the aim of this study was to evaluate whether bovine IL-10 has immunomodulatory activities on human monocytes and dendritic cells. Peripheral blood monocytes were isolated from healthy donors, and used directly or allowed to differentiate to dendritic cells under the influence of IL-4 and GM-CSF. Recombinant bovine IL-10 inhibited TLR induced activation of monocytes, and dose-dependently inhibited LPS-induced activation of monocyte-derived DCs comparable to human IL-10. By using blocking antibodies to either bovine IL-10 or the human IL-10 receptor it was demonstrated that inhibition of monocyte activation by bovine IL-10 was dependent on binding of bovine IL-10 to the human IL-10R. These data demonstrate that bovine IL-10 potently inhibits the activation of human myeloid cells in response to TLR activation. Bovine IL-10 present in dairy products may thus potentially contribute to the prevention of necrotizing enterocolitis and allergy, enhance mucosal tolerance induction and decrease intestinal inflammation and may therefore be applicable in infant foods and in immunomodulatory diets.
format	article
author	Gerco den Hartog Huub F J Savelkoul Ruud Schoemaker Edwin Tijhaar Adrie H Westphal Talitha de Ruiter Elise van de Weg-Schrijver R J Joost van Neerven
author_facet	Gerco den Hartog Huub F J Savelkoul Ruud Schoemaker Edwin Tijhaar Adrie H Westphal Talitha de Ruiter Elise van de Weg-Schrijver R J Joost van Neerven
author_sort	Gerco den Hartog
title	Modulation of human immune responses by bovine interleukin-10.
title_short	Modulation of human immune responses by bovine interleukin-10.
title_full	Modulation of human immune responses by bovine interleukin-10.
title_fullStr	Modulation of human immune responses by bovine interleukin-10.
title_full_unstemmed	Modulation of human immune responses by bovine interleukin-10.
title_sort	modulation of human immune responses by bovine interleukin-10.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/ba36882c9a20402f9b561788c72c2b7c
work_keys_str_mv	AT gercodenhartog modulationofhumanimmuneresponsesbybovineinterleukin10 AT huubfjsavelkoul modulationofhumanimmuneresponsesbybovineinterleukin10 AT ruudschoemaker modulationofhumanimmuneresponsesbybovineinterleukin10 AT edwintijhaar modulationofhumanimmuneresponsesbybovineinterleukin10 AT adriehwestphal modulationofhumanimmuneresponsesbybovineinterleukin10 AT talithaderuiter modulationofhumanimmuneresponsesbybovineinterleukin10 AT elisevandewegschrijver modulationofhumanimmuneresponsesbybovineinterleukin10 AT rjjoostvanneerven modulationofhumanimmuneresponsesbybovineinterleukin10
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Modulation of human immune responses by bovine interleukin-10.

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