Short Communication: Genetic diversity among Fusarium isolates from cereals in Iran assessed using RAPD marker

Siahpoush S, Darvishnia M. 2019. Short Communication: Genetic diversity among Fusarium isolates from cereals in Iran assessed using RAPD marker. Biodiversitas 20: 292-296. Fusarium species cause important disease on many crops including cereals, and accurate identification and then proper management...

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Autores principales:	SARA SIAHPOUSH, MOSTAFA DARVISHNIA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	fusarium genetic diversity rapd translation elongation factor 1-alpha upgma Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ba36b6c2f7b044de8f36d09722109bc8
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spelling	oai:doaj.org-article:ba36b6c2f7b044de8f36d09722109bc82021-11-16T14:06:32ZShort Communication: Genetic diversity among Fusarium isolates from cereals in Iran assessed using RAPD marker1412-033X2085-472210.13057/biodiv/d200133https://doaj.org/article/ba36b6c2f7b044de8f36d09722109bc82018-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3419https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Siahpoush S, Darvishnia M. 2019. Short Communication: Genetic diversity among Fusarium isolates from cereals in Iran assessed using RAPD marker. Biodiversitas 20: 292-296. Fusarium species cause important disease on many crops including cereals, and accurate identification and then proper management of this disease will be helpful to reduce economic losses. In this paper, 13 Fusarium isolates from root and crown of cereals in western Iran were identified by morphological and molecular methods. Sequencing of translation elongation factor 1-alpha revealed 8 species as follows: Fusarium acuminatum, F. avenaceum, F. culmorum, F. equiseti, F. proliferatum, F. reticulatum, F. solani, and F. tricinctum. Species diversity was analyzed by random amplified polymorphic DNA (RAPD). Four primers were used as Rfu9, Rfu10, Rfu23, and Rfu25 which all of them produced distinct and reproducible bands. A dendrogram was developed by UPGMA. Generated polymorphic bands were observed in all 13 different species by 4 primers. Rfu9 by 13 bands and Rfu23 by 10 ones, produced the most and the least bands respectively. Genetic similarity coefficient was between 0.00-0.9. RAPD analysis showed that these isolates were genetically varied and two clusters were formed with Fusarium isolates.SARA SIAHPOUSHMOSTAFA DARVISHNIAMBI & UNS Soloarticlefusariumgenetic diversityrapdtranslation elongation factor 1-alphaupgmaBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 1, Pp 292-296 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	fusarium genetic diversity rapd translation elongation factor 1-alpha upgma Biology (General) QH301-705.5
spellingShingle	fusarium genetic diversity rapd translation elongation factor 1-alpha upgma Biology (General) QH301-705.5 SARA SIAHPOUSH MOSTAFA DARVISHNIA Short Communication: Genetic diversity among Fusarium isolates from cereals in Iran assessed using RAPD marker
description	Siahpoush S, Darvishnia M. 2019. Short Communication: Genetic diversity among Fusarium isolates from cereals in Iran assessed using RAPD marker. Biodiversitas 20: 292-296. Fusarium species cause important disease on many crops including cereals, and accurate identification and then proper management of this disease will be helpful to reduce economic losses. In this paper, 13 Fusarium isolates from root and crown of cereals in western Iran were identified by morphological and molecular methods. Sequencing of translation elongation factor 1-alpha revealed 8 species as follows: Fusarium acuminatum, F. avenaceum, F. culmorum, F. equiseti, F. proliferatum, F. reticulatum, F. solani, and F. tricinctum. Species diversity was analyzed by random amplified polymorphic DNA (RAPD). Four primers were used as Rfu9, Rfu10, Rfu23, and Rfu25 which all of them produced distinct and reproducible bands. A dendrogram was developed by UPGMA. Generated polymorphic bands were observed in all 13 different species by 4 primers. Rfu9 by 13 bands and Rfu23 by 10 ones, produced the most and the least bands respectively. Genetic similarity coefficient was between 0.00-0.9. RAPD analysis showed that these isolates were genetically varied and two clusters were formed with Fusarium isolates.
format	article
author	SARA SIAHPOUSH MOSTAFA DARVISHNIA
author_facet	SARA SIAHPOUSH MOSTAFA DARVISHNIA
author_sort	SARA SIAHPOUSH
title	Short Communication: Genetic diversity among Fusarium isolates from cereals in Iran assessed using RAPD marker
title_short	Short Communication: Genetic diversity among Fusarium isolates from cereals in Iran assessed using RAPD marker
title_full	Short Communication: Genetic diversity among Fusarium isolates from cereals in Iran assessed using RAPD marker
title_fullStr	Short Communication: Genetic diversity among Fusarium isolates from cereals in Iran assessed using RAPD marker
title_full_unstemmed	Short Communication: Genetic diversity among Fusarium isolates from cereals in Iran assessed using RAPD marker
title_sort	short communication: genetic diversity among fusarium isolates from cereals in iran assessed using rapd marker
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/ba36b6c2f7b044de8f36d09722109bc8
work_keys_str_mv	AT sarasiahpoush shortcommunicationgeneticdiversityamongfusariumisolatesfromcerealsiniranassessedusingrapdmarker AT mostafadarvishnia shortcommunicationgeneticdiversityamongfusariumisolatesfromcerealsiniranassessedusingrapdmarker
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Short Communication: Genetic diversity among Fusarium isolates from cereals in Iran assessed using RAPD marker

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