Pollination compatibility of Dendrobium spp. orchids from Bali, Indonesia, and the effects of adding organic matters on seed germination under in vitro culture

Abstract. Darmawati IAP, Astarini IA, Yuswanti H, Fitriani Y. 2021. Conservation of Dendrobium spp. Bali orchids (Indonesia) through in vitro seed culture. Biodiversitas 22: 2554-2559. Orchid species, including Dendrobium spp., originated from Bali are threatened to extinction due to habitat destruc...

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Autores principales:	IDA AYU PUTRI DARMAWATI, IDA AYU ASTARINI, HESTIN YUSWANTI, YUYUN FITRIANI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	compatibility, pollination, propagation, organic matters Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ba45544feaa54469ad0da4347b976bbc
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spelling	oai:doaj.org-article:ba45544feaa54469ad0da4347b976bbc2021-11-22T12:13:36ZPollination compatibility of Dendrobium spp. orchids from Bali, Indonesia, and the effects of adding organic matters on seed germination under in vitro culture1412-033X2085-472210.13057/biodiv/d220513https://doaj.org/article/ba45544feaa54469ad0da4347b976bbc2021-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/8018https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Darmawati IAP, Astarini IA, Yuswanti H, Fitriani Y. 2021. Conservation of Dendrobium spp. Bali orchids (Indonesia) through in vitro seed culture. Biodiversitas 22: 2554-2559. Orchid species, including Dendrobium spp., originated from Bali are threatened to extinction due to habitat destruction, illegal hunting, small population size, and the effects of global environmental change. Conservation strategies are therefore required to preserve the remaining germplasm, one of which is through in vitro seed propagation. This research aimed to investigate the pollination compatibility (i.e., pollination that produce fruits) of Dendrobium orchids originated from Bali, and to determine the effects of adding organic matters (i.e., coconut water, tomato extract, and peptone) to the base media on orchid seed germination under in vitro culture condition. Self-pollination and sibling pollination were implemented to seven orchid species, namely Dendrobium macrophyllum, D. heterocarpum, D. fimbriatum, D. linearifolium, D. spathilingue, D. secundum, and D. plicatile. The results showed that only four species, i.e., D. macrophyllum, D. heterocarpum, D. fimbriatum, and D. linearifolium, that produced fruits after being pollinated. The subsequent in vitro propagation of seeds produced by the four species showed that the addition of organic matters of tomato extract, coconut water, and peptone significantly affected the germination of Dendrobium forma Bali orchid seeds, indicated by different germination rates. The addition of 20% coconut water to Vacin and Went (VW) media gave the highest percentages of seed germination and protocorm formation for D. macrophyllum, D. heterocarpum, and D. fimbriatum at 98.33% and 95.00%; 91.67% and 91.67%; and 95% and 98.33%, respectively. Our findings can serve as baseline information when developing conservation strategies of Dendrobium orchids from Bali, particularly from the aspect of propagationIDA AYU PUTRI DARMAWATIIDA AYU ASTARINIHESTIN YUSWANTIYUYUN FITRIANIMBI & UNS Soloarticlecompatibility, pollination, propagation, organic matters Biology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	compatibility, pollination, propagation, organic matters Biology (General) QH301-705.5
spellingShingle	compatibility, pollination, propagation, organic matters Biology (General) QH301-705.5 IDA AYU PUTRI DARMAWATI IDA AYU ASTARINI HESTIN YUSWANTI YUYUN FITRIANI Pollination compatibility of Dendrobium spp. orchids from Bali, Indonesia, and the effects of adding organic matters on seed germination under in vitro culture
description	Abstract. Darmawati IAP, Astarini IA, Yuswanti H, Fitriani Y. 2021. Conservation of Dendrobium spp. Bali orchids (Indonesia) through in vitro seed culture. Biodiversitas 22: 2554-2559. Orchid species, including Dendrobium spp., originated from Bali are threatened to extinction due to habitat destruction, illegal hunting, small population size, and the effects of global environmental change. Conservation strategies are therefore required to preserve the remaining germplasm, one of which is through in vitro seed propagation. This research aimed to investigate the pollination compatibility (i.e., pollination that produce fruits) of Dendrobium orchids originated from Bali, and to determine the effects of adding organic matters (i.e., coconut water, tomato extract, and peptone) to the base media on orchid seed germination under in vitro culture condition. Self-pollination and sibling pollination were implemented to seven orchid species, namely Dendrobium macrophyllum, D. heterocarpum, D. fimbriatum, D. linearifolium, D. spathilingue, D. secundum, and D. plicatile. The results showed that only four species, i.e., D. macrophyllum, D. heterocarpum, D. fimbriatum, and D. linearifolium, that produced fruits after being pollinated. The subsequent in vitro propagation of seeds produced by the four species showed that the addition of organic matters of tomato extract, coconut water, and peptone significantly affected the germination of Dendrobium forma Bali orchid seeds, indicated by different germination rates. The addition of 20% coconut water to Vacin and Went (VW) media gave the highest percentages of seed germination and protocorm formation for D. macrophyllum, D. heterocarpum, and D. fimbriatum at 98.33% and 95.00%; 91.67% and 91.67%; and 95% and 98.33%, respectively. Our findings can serve as baseline information when developing conservation strategies of Dendrobium orchids from Bali, particularly from the aspect of propagation
format	article
author	IDA AYU PUTRI DARMAWATI IDA AYU ASTARINI HESTIN YUSWANTI YUYUN FITRIANI
author_facet	IDA AYU PUTRI DARMAWATI IDA AYU ASTARINI HESTIN YUSWANTI YUYUN FITRIANI
author_sort	IDA AYU PUTRI DARMAWATI
title	Pollination compatibility of Dendrobium spp. orchids from Bali, Indonesia, and the effects of adding organic matters on seed germination under in vitro culture
title_short	Pollination compatibility of Dendrobium spp. orchids from Bali, Indonesia, and the effects of adding organic matters on seed germination under in vitro culture
title_full	Pollination compatibility of Dendrobium spp. orchids from Bali, Indonesia, and the effects of adding organic matters on seed germination under in vitro culture
title_fullStr	Pollination compatibility of Dendrobium spp. orchids from Bali, Indonesia, and the effects of adding organic matters on seed germination under in vitro culture
title_full_unstemmed	Pollination compatibility of Dendrobium spp. orchids from Bali, Indonesia, and the effects of adding organic matters on seed germination under in vitro culture
title_sort	pollination compatibility of dendrobium spp. orchids from bali, indonesia, and the effects of adding organic matters on seed germination under in vitro culture
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/ba45544feaa54469ad0da4347b976bbc
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Pollination compatibility of Dendrobium spp. orchids from Bali, Indonesia, and the effects of adding organic matters on seed germination under in vitro culture

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