Dual pili post-translational modifications synergize to mediate meningococcal adherence to platelet activating factor receptor on human airway cells.

Pili of pathogenic Neisseria are major virulence factors associated with adhesion, twitching motility, auto-aggregation, and DNA transformation. Pili of N. meningitidis are subject to several different post-translational modifications. Among these pilin modifications, the presence of phosphorylcholi...

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Autores principales:	Freda E-C Jen, Matthew J Warren, Benjamin L Schulz, Peter M Power, W Edward Swords, Jeffery N Weiser, Michael A Apicella, Jennifer L Edwards, Michael P Jennings
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ba46c9c302e347529caa330cdba32102
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spelling	oai:doaj.org-article:ba46c9c302e347529caa330cdba321022021-11-18T06:05:37ZDual pili post-translational modifications synergize to mediate meningococcal adherence to platelet activating factor receptor on human airway cells.1553-73661553-737410.1371/journal.ppat.1003377https://doaj.org/article/ba46c9c302e347529caa330cdba321022013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23696740/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Pili of pathogenic Neisseria are major virulence factors associated with adhesion, twitching motility, auto-aggregation, and DNA transformation. Pili of N. meningitidis are subject to several different post-translational modifications. Among these pilin modifications, the presence of phosphorylcholine (ChoP) and a glycan on the pilin protein are phase-variable (subject to high frequency, reversible on/off switching of expression). In this study we report the location of two ChoP modifications on the C-terminus of N. meningitidis pilin. We show that the surface accessibility of ChoP on pili is affected by phase variable changes to the structure of the pilin-linked glycan. We identify for the first time that the platelet activating factor receptor (PAFr) is a key, early event receptor for meningococcal adherence to human bronchial epithelial cells and tissue, and that synergy between the pilin-linked glycan and ChoP post-translational modifications is required for pili to optimally engage PAFr to mediate adherence to human airway cells.Freda E-C JenMatthew J WarrenBenjamin L SchulzPeter M PowerW Edward SwordsJeffery N WeiserMichael A ApicellaJennifer L EdwardsMichael P JenningsPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 5, p e1003377 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Freda E-C Jen Matthew J Warren Benjamin L Schulz Peter M Power W Edward Swords Jeffery N Weiser Michael A Apicella Jennifer L Edwards Michael P Jennings Dual pili post-translational modifications synergize to mediate meningococcal adherence to platelet activating factor receptor on human airway cells.
description	Pili of pathogenic Neisseria are major virulence factors associated with adhesion, twitching motility, auto-aggregation, and DNA transformation. Pili of N. meningitidis are subject to several different post-translational modifications. Among these pilin modifications, the presence of phosphorylcholine (ChoP) and a glycan on the pilin protein are phase-variable (subject to high frequency, reversible on/off switching of expression). In this study we report the location of two ChoP modifications on the C-terminus of N. meningitidis pilin. We show that the surface accessibility of ChoP on pili is affected by phase variable changes to the structure of the pilin-linked glycan. We identify for the first time that the platelet activating factor receptor (PAFr) is a key, early event receptor for meningococcal adherence to human bronchial epithelial cells and tissue, and that synergy between the pilin-linked glycan and ChoP post-translational modifications is required for pili to optimally engage PAFr to mediate adherence to human airway cells.
format	article
author	Freda E-C Jen Matthew J Warren Benjamin L Schulz Peter M Power W Edward Swords Jeffery N Weiser Michael A Apicella Jennifer L Edwards Michael P Jennings
author_facet	Freda E-C Jen Matthew J Warren Benjamin L Schulz Peter M Power W Edward Swords Jeffery N Weiser Michael A Apicella Jennifer L Edwards Michael P Jennings
author_sort	Freda E-C Jen
title	Dual pili post-translational modifications synergize to mediate meningococcal adherence to platelet activating factor receptor on human airway cells.
title_short	Dual pili post-translational modifications synergize to mediate meningococcal adherence to platelet activating factor receptor on human airway cells.
title_full	Dual pili post-translational modifications synergize to mediate meningococcal adherence to platelet activating factor receptor on human airway cells.
title_fullStr	Dual pili post-translational modifications synergize to mediate meningococcal adherence to platelet activating factor receptor on human airway cells.
title_full_unstemmed	Dual pili post-translational modifications synergize to mediate meningococcal adherence to platelet activating factor receptor on human airway cells.
title_sort	dual pili post-translational modifications synergize to mediate meningococcal adherence to platelet activating factor receptor on human airway cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ba46c9c302e347529caa330cdba32102
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Dual pili post-translational modifications synergize to mediate meningococcal adherence to platelet activating factor receptor on human airway cells.

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