Metabolome analysis revealed that soybean-Aspergillus oryzae interaction induced dynamic metabolic and daidzein prenylation changes.

Several isoflavonoids are well known for their ability to act as soybean phytoalexins. However, the overall effects of the soybean-Aspergillus oryzae interaction on metabolism remain largely unknown. The aim of this study is to reveal an overview of nutritive and metabolic changes in germinated and...

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Autores principales:	Haruya Takahashi, Koji Ochiai, Kuni Sasaki, Atsushi Izumi, Yu Shinyama, Shinsuke Mohri, Wataru Nomura, Huei-Fen Jheng, Teruo Kawada, Kazuo Inoue, Tsuyoshi Goto
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ba4ddc2c67f94cb287bd06e0f594f5a8
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spelling	oai:doaj.org-article:ba4ddc2c67f94cb287bd06e0f594f5a82021-12-02T20:15:40ZMetabolome analysis revealed that soybean-Aspergillus oryzae interaction induced dynamic metabolic and daidzein prenylation changes.1932-620310.1371/journal.pone.0254190https://doaj.org/article/ba4ddc2c67f94cb287bd06e0f594f5a82021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254190https://doaj.org/toc/1932-6203Several isoflavonoids are well known for their ability to act as soybean phytoalexins. However, the overall effects of the soybean-Aspergillus oryzae interaction on metabolism remain largely unknown. The aim of this study is to reveal an overview of nutritive and metabolic changes in germinated and A. oryzae-elicited soybeans. The levels of individual nutrients were measured using the ustulation, ashing, Kjeldahl, and Folch methods. The levels of individual amino acids were measured using high-performance liquid chromatography. Low-molecular-weight compounds were measured through metabolome analysis using liquid chromatography-mass spectrometry. Although the levels of individual nutrients and amino acids were strongly influenced by the germination process, the elicitation process had little effect on the change in the contents of individual nutrients and amino acids. However, after analyzing approximately 700 metabolites using metabolome analysis, we found that the levels of many of the metabolites were strongly influenced by soybean-A. oryzae interactions. In particular, the data indicate that steroid, terpenoid, phenylpropanoid, flavonoid, and fatty acid metabolism were influenced by the elicitation process. Furthermore, we demonstrated that not the germination process but the elicitation process induced daidzein prenylation, suggesting that the soybean-A. oryzae interactions produce various phytoalexins that are valuable for health promotion and/or disease prevention.Haruya TakahashiKoji OchiaiKuni SasakiAtsushi IzumiYu ShinyamaShinsuke MohriWataru NomuraHuei-Fen JhengTeruo KawadaKazuo InoueTsuyoshi GotoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254190 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haruya Takahashi Koji Ochiai Kuni Sasaki Atsushi Izumi Yu Shinyama Shinsuke Mohri Wataru Nomura Huei-Fen Jheng Teruo Kawada Kazuo Inoue Tsuyoshi Goto Metabolome analysis revealed that soybean-Aspergillus oryzae interaction induced dynamic metabolic and daidzein prenylation changes.
description	Several isoflavonoids are well known for their ability to act as soybean phytoalexins. However, the overall effects of the soybean-Aspergillus oryzae interaction on metabolism remain largely unknown. The aim of this study is to reveal an overview of nutritive and metabolic changes in germinated and A. oryzae-elicited soybeans. The levels of individual nutrients were measured using the ustulation, ashing, Kjeldahl, and Folch methods. The levels of individual amino acids were measured using high-performance liquid chromatography. Low-molecular-weight compounds were measured through metabolome analysis using liquid chromatography-mass spectrometry. Although the levels of individual nutrients and amino acids were strongly influenced by the germination process, the elicitation process had little effect on the change in the contents of individual nutrients and amino acids. However, after analyzing approximately 700 metabolites using metabolome analysis, we found that the levels of many of the metabolites were strongly influenced by soybean-A. oryzae interactions. In particular, the data indicate that steroid, terpenoid, phenylpropanoid, flavonoid, and fatty acid metabolism were influenced by the elicitation process. Furthermore, we demonstrated that not the germination process but the elicitation process induced daidzein prenylation, suggesting that the soybean-A. oryzae interactions produce various phytoalexins that are valuable for health promotion and/or disease prevention.
format	article
author	Haruya Takahashi Koji Ochiai Kuni Sasaki Atsushi Izumi Yu Shinyama Shinsuke Mohri Wataru Nomura Huei-Fen Jheng Teruo Kawada Kazuo Inoue Tsuyoshi Goto
author_facet	Haruya Takahashi Koji Ochiai Kuni Sasaki Atsushi Izumi Yu Shinyama Shinsuke Mohri Wataru Nomura Huei-Fen Jheng Teruo Kawada Kazuo Inoue Tsuyoshi Goto
author_sort	Haruya Takahashi
title	Metabolome analysis revealed that soybean-Aspergillus oryzae interaction induced dynamic metabolic and daidzein prenylation changes.
title_short	Metabolome analysis revealed that soybean-Aspergillus oryzae interaction induced dynamic metabolic and daidzein prenylation changes.
title_full	Metabolome analysis revealed that soybean-Aspergillus oryzae interaction induced dynamic metabolic and daidzein prenylation changes.
title_fullStr	Metabolome analysis revealed that soybean-Aspergillus oryzae interaction induced dynamic metabolic and daidzein prenylation changes.
title_full_unstemmed	Metabolome analysis revealed that soybean-Aspergillus oryzae interaction induced dynamic metabolic and daidzein prenylation changes.
title_sort	metabolome analysis revealed that soybean-aspergillus oryzae interaction induced dynamic metabolic and daidzein prenylation changes.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/ba4ddc2c67f94cb287bd06e0f594f5a8
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Metabolome analysis revealed that soybean-Aspergillus oryzae interaction induced dynamic metabolic and daidzein prenylation changes.

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