Molecular diagnostics for verification of pleural tuberculosis in Morocco

Pleural tuberculosis (pTB) is a very common form of extrapulmonary tuberculosis (TB). pTB diagnostics represents a major burning challenge worldwide due to the limitations of available conventional diagnostic tools. These latter include microscopic examination of the pleural fluid for acid-fast baci...

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Autores principales:	I. Chaoui, S. Taoudi, A. Oudghiri, J. Benamor, J. Bourkadi, M. El Mzibri
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2021
Materias:	туберкулезный плеврит mycobacterium tuberculosis полимеразная цепная реакция is6110 внутренний транскрибируемый спейсер микобактерий (myits) Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/ba4e787f7bc94d0693eb7f61290db186
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spelling	oai:doaj.org-article:ba4e787f7bc94d0693eb7f61290db1862021-11-22T07:09:54ZMolecular diagnostics for verification of pleural tuberculosis in Morocco2220-76192313-739810.15789/2220-7619-MDO-1399https://doaj.org/article/ba4e787f7bc94d0693eb7f61290db1862021-06-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1399https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Pleural tuberculosis (pTB) is a very common form of extrapulmonary tuberculosis (TB). pTB diagnostics represents a major burning challenge worldwide due to the limitations of available conventional diagnostic tools. These latter include microscopic examination of the pleural fluid for acid-fast bacilli, mycobacterial culture of pleural fluid in solid or liquid media, sputum or pleural tissue, and histopathological examination of pleural tissue; these tests have recognized limitations for clinical use. Hence, to overcome these limitations, attention has been devoted to new nucleic acid amplification (NAA) diagnostic tests such as the polymerase chain reaction (PCR) and real-time PCR (RT-PCR), owing to their accuracy, rapidity, high sensitivity and specificity. Within this context, this prospective study was conducted to evaluate the performance of molecular diagnosis methods for differentiation between tuberculosis and non-tuberculosis pleural effusions. Fifty patients with pleural effusion were enrolled in this prospective study in Rabat, Morocco. The efficacy of conventional polymerase chain reaction (PCR) in the diagnostics of tuberculous pleurisy by targeting IS6110 and mycobacterial internal transcribed spacer (MYITS) was evaluated compared to histopathologic examination and culture data. Our results showed that IS6110 PCR could “rule in” pTB, the sensitivity and specificity being 41.6% and 85.7%, respectively. Therefore, the findings confirmed that molecular tests exert a relatively high specificity in EPTB but lower sensitivity, thus a positive test is considered as a pTB case whereas negative one cannot exclude the disease. Although the study was limited by a small sample size, it adds to the body of evidence of usefulness of molecular testing as adjuncts to histopathologic examination for accurate diagnosis of pTB, to treat timely and to avoid the emergence and spread of drug resistant pTB. However, further efforts should be made to increase the sensitivity of NAA methods and to identify the best molecular targets to be useful in clinical practice.I. ChaouiS. TaoudiA. OudghiriJ. BenamorJ. BourkadiM. El MzibriSankt-Peterburg : NIIÈM imeni Pasteraarticleтуберкулезный плевритmycobacterium tuberculosisполимеразная цепная реакцияis6110внутренний транскрибируемый спейсер микобактерий (myits)Infectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 11, Iss 3, Pp 491-496 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	туберкулезный плеврит mycobacterium tuberculosis полимеразная цепная реакция is6110 внутренний транскрибируемый спейсер микобактерий (myits) Infectious and parasitic diseases RC109-216
spellingShingle	туберкулезный плеврит mycobacterium tuberculosis полимеразная цепная реакция is6110 внутренний транскрибируемый спейсер микобактерий (myits) Infectious and parasitic diseases RC109-216 I. Chaoui S. Taoudi A. Oudghiri J. Benamor J. Bourkadi M. El Mzibri Molecular diagnostics for verification of pleural tuberculosis in Morocco
description	Pleural tuberculosis (pTB) is a very common form of extrapulmonary tuberculosis (TB). pTB diagnostics represents a major burning challenge worldwide due to the limitations of available conventional diagnostic tools. These latter include microscopic examination of the pleural fluid for acid-fast bacilli, mycobacterial culture of pleural fluid in solid or liquid media, sputum or pleural tissue, and histopathological examination of pleural tissue; these tests have recognized limitations for clinical use. Hence, to overcome these limitations, attention has been devoted to new nucleic acid amplification (NAA) diagnostic tests such as the polymerase chain reaction (PCR) and real-time PCR (RT-PCR), owing to their accuracy, rapidity, high sensitivity and specificity. Within this context, this prospective study was conducted to evaluate the performance of molecular diagnosis methods for differentiation between tuberculosis and non-tuberculosis pleural effusions. Fifty patients with pleural effusion were enrolled in this prospective study in Rabat, Morocco. The efficacy of conventional polymerase chain reaction (PCR) in the diagnostics of tuberculous pleurisy by targeting IS6110 and mycobacterial internal transcribed spacer (MYITS) was evaluated compared to histopathologic examination and culture data. Our results showed that IS6110 PCR could “rule in” pTB, the sensitivity and specificity being 41.6% and 85.7%, respectively. Therefore, the findings confirmed that molecular tests exert a relatively high specificity in EPTB but lower sensitivity, thus a positive test is considered as a pTB case whereas negative one cannot exclude the disease. Although the study was limited by a small sample size, it adds to the body of evidence of usefulness of molecular testing as adjuncts to histopathologic examination for accurate diagnosis of pTB, to treat timely and to avoid the emergence and spread of drug resistant pTB. However, further efforts should be made to increase the sensitivity of NAA methods and to identify the best molecular targets to be useful in clinical practice.
format	article
author	I. Chaoui S. Taoudi A. Oudghiri J. Benamor J. Bourkadi M. El Mzibri
author_facet	I. Chaoui S. Taoudi A. Oudghiri J. Benamor J. Bourkadi M. El Mzibri
author_sort	I. Chaoui
title	Molecular diagnostics for verification of pleural tuberculosis in Morocco
title_short	Molecular diagnostics for verification of pleural tuberculosis in Morocco
title_full	Molecular diagnostics for verification of pleural tuberculosis in Morocco
title_fullStr	Molecular diagnostics for verification of pleural tuberculosis in Morocco
title_full_unstemmed	Molecular diagnostics for verification of pleural tuberculosis in Morocco
title_sort	molecular diagnostics for verification of pleural tuberculosis in morocco
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2021
url	https://doaj.org/article/ba4e787f7bc94d0693eb7f61290db186
work_keys_str_mv	AT ichaoui moleculardiagnosticsforverificationofpleuraltuberculosisinmorocco AT staoudi moleculardiagnosticsforverificationofpleuraltuberculosisinmorocco AT aoudghiri moleculardiagnosticsforverificationofpleuraltuberculosisinmorocco AT jbenamor moleculardiagnosticsforverificationofpleuraltuberculosisinmorocco AT jbourkadi moleculardiagnosticsforverificationofpleuraltuberculosisinmorocco AT melmzibri moleculardiagnosticsforverificationofpleuraltuberculosisinmorocco
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Molecular diagnostics for verification of pleural tuberculosis in Morocco

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