Modulation of gene transcription and epigenetics of colon carcinoma cells by bacterial membrane vesicles

Abstract Interactions between bacteria and colon cancer cells influence the transcription of the host cell. Yet is it undetermined whether the bacteria itself or the communication between the host and bacteria is responsible for the genomic changes in the eukaryotic cell. Now, we have investigated t...

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Autores principales:	Svitlana Vdovikova, Siv Gilfillan, Shixiong Wang, Mitesh Dongre, Sun Nyunt Wai, Antoni Hurtado
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ba567a9041144180b44589ed1f958642
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spelling	oai:doaj.org-article:ba567a9041144180b44589ed1f9586422021-12-02T12:31:56ZModulation of gene transcription and epigenetics of colon carcinoma cells by bacterial membrane vesicles10.1038/s41598-018-25308-92045-2322https://doaj.org/article/ba567a9041144180b44589ed1f9586422018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25308-9https://doaj.org/toc/2045-2322Abstract Interactions between bacteria and colon cancer cells influence the transcription of the host cell. Yet is it undetermined whether the bacteria itself or the communication between the host and bacteria is responsible for the genomic changes in the eukaryotic cell. Now, we have investigated the genomic and epigenetic consequences of co-culturing colorectal carcinoma cells with membrane vesicles from pathogenic bacteria Vibrio cholerae and non-pathogenic commensal bacteria Escherichia coli. Our study reveals that membrane vesicles from pathogenic and commensal bacteria have a global impact on the gene expression of colon-carcinoma cells. The changes in gene expression correlate positively with both epigenetic changes and chromatin accessibility of promoters at transcription start sites of genes induced by both types of membrane vesicles. Moreover, we have demonstrated that membrane vesicles obtained only from V. cholerae induced the expression of genes associated with epithelial cell differentiation. Altogether, our study suggests that the observed genomic changes in host cells might be due to specific components of membrane vesicles and do not require communication by direct contact with the bacteria.Svitlana VdovikovaSiv GilfillanShixiong WangMitesh DongreSun Nyunt WaiAntoni HurtadoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Svitlana Vdovikova Siv Gilfillan Shixiong Wang Mitesh Dongre Sun Nyunt Wai Antoni Hurtado Modulation of gene transcription and epigenetics of colon carcinoma cells by bacterial membrane vesicles
description	Abstract Interactions between bacteria and colon cancer cells influence the transcription of the host cell. Yet is it undetermined whether the bacteria itself or the communication between the host and bacteria is responsible for the genomic changes in the eukaryotic cell. Now, we have investigated the genomic and epigenetic consequences of co-culturing colorectal carcinoma cells with membrane vesicles from pathogenic bacteria Vibrio cholerae and non-pathogenic commensal bacteria Escherichia coli. Our study reveals that membrane vesicles from pathogenic and commensal bacteria have a global impact on the gene expression of colon-carcinoma cells. The changes in gene expression correlate positively with both epigenetic changes and chromatin accessibility of promoters at transcription start sites of genes induced by both types of membrane vesicles. Moreover, we have demonstrated that membrane vesicles obtained only from V. cholerae induced the expression of genes associated with epithelial cell differentiation. Altogether, our study suggests that the observed genomic changes in host cells might be due to specific components of membrane vesicles and do not require communication by direct contact with the bacteria.
format	article
author	Svitlana Vdovikova Siv Gilfillan Shixiong Wang Mitesh Dongre Sun Nyunt Wai Antoni Hurtado
author_facet	Svitlana Vdovikova Siv Gilfillan Shixiong Wang Mitesh Dongre Sun Nyunt Wai Antoni Hurtado
author_sort	Svitlana Vdovikova
title	Modulation of gene transcription and epigenetics of colon carcinoma cells by bacterial membrane vesicles
title_short	Modulation of gene transcription and epigenetics of colon carcinoma cells by bacterial membrane vesicles
title_full	Modulation of gene transcription and epigenetics of colon carcinoma cells by bacterial membrane vesicles
title_fullStr	Modulation of gene transcription and epigenetics of colon carcinoma cells by bacterial membrane vesicles
title_full_unstemmed	Modulation of gene transcription and epigenetics of colon carcinoma cells by bacterial membrane vesicles
title_sort	modulation of gene transcription and epigenetics of colon carcinoma cells by bacterial membrane vesicles
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/ba567a9041144180b44589ed1f958642
work_keys_str_mv	AT svitlanavdovikova modulationofgenetranscriptionandepigeneticsofcoloncarcinomacellsbybacterialmembranevesicles AT sivgilfillan modulationofgenetranscriptionandepigeneticsofcoloncarcinomacellsbybacterialmembranevesicles AT shixiongwang modulationofgenetranscriptionandepigeneticsofcoloncarcinomacellsbybacterialmembranevesicles AT miteshdongre modulationofgenetranscriptionandepigeneticsofcoloncarcinomacellsbybacterialmembranevesicles AT sunnyuntwai modulationofgenetranscriptionandepigeneticsofcoloncarcinomacellsbybacterialmembranevesicles AT antonihurtado modulationofgenetranscriptionandepigeneticsofcoloncarcinomacellsbybacterialmembranevesicles
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Modulation of gene transcription and epigenetics of colon carcinoma cells by bacterial membrane vesicles

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