Unactivated leukocyte expression of C-reactive protein is minimal and not dependent on rs1205 genotype

Abstract C-reactive protein (CRP), a prominent component of the innate immune system, is implicated in the pathophysiology of many conditions. CRP production primarily occurs in the liver; but contributions from other tissues is unclear. The Genotype-Tissue Expression Portal shows essentially no exp...

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Autores principales:	L. G. Best, C. Azure, K. Martell, K. S. Tsosie, B. Voels
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ba583cfdc0244f0b916eaafced98f1a0
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spelling	oai:doaj.org-article:ba583cfdc0244f0b916eaafced98f1a02021-12-02T15:54:10ZUnactivated leukocyte expression of C-reactive protein is minimal and not dependent on rs1205 genotype10.1038/s41598-021-85272-92045-2322https://doaj.org/article/ba583cfdc0244f0b916eaafced98f1a02021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85272-9https://doaj.org/toc/2045-2322Abstract C-reactive protein (CRP), a prominent component of the innate immune system, is implicated in the pathophysiology of many conditions. CRP production primarily occurs in the liver; but contributions from other tissues is unclear. The Genotype-Tissue Expression Portal shows essentially no expression in whole blood and reports in the literature are conflicting. Multiple genomic variants influence serum levels of CRP. We measured CRP mRNA expression in leukocytes and sought to determine if rs1205 genotype influences leukocyte expression. Leukocytes were obtained from 20 women differing by genotype. Quantitative, real-time PCR (RT-qPCR) detected CRP and reference gene (GAPDH) mRNA. Leukocyte expression was calculated by the 2ΔCT method, and against a standard curve. Digital drop PCR was also used to calculate expression ratios. Student's t test and linear regression methods examined possible differences between genotypes. During 32 runs (10 replicates each), the RT-qPCR mean (SD) CRP/GAPDH ratio was 3.39 × 10–4 (SD 1.73 × 10–4) and 3.15 × 10–4 (SD 1.64 × 10–4) for TT and CC genotypes respectively, p = 0.76; and digital drop PCR results were similar. Serum CRP was not significantly different between genotypes, nor correlated with leukocyte expression. CRP is minimally expressed in unactivated leukocytes and this expression is not likely influenced by rs1205 genotype.L. G. BestC. AzureK. MartellK. S. TsosieB. VoelsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q L. G. Best C. Azure K. Martell K. S. Tsosie B. Voels Unactivated leukocyte expression of C-reactive protein is minimal and not dependent on rs1205 genotype
description	Abstract C-reactive protein (CRP), a prominent component of the innate immune system, is implicated in the pathophysiology of many conditions. CRP production primarily occurs in the liver; but contributions from other tissues is unclear. The Genotype-Tissue Expression Portal shows essentially no expression in whole blood and reports in the literature are conflicting. Multiple genomic variants influence serum levels of CRP. We measured CRP mRNA expression in leukocytes and sought to determine if rs1205 genotype influences leukocyte expression. Leukocytes were obtained from 20 women differing by genotype. Quantitative, real-time PCR (RT-qPCR) detected CRP and reference gene (GAPDH) mRNA. Leukocyte expression was calculated by the 2ΔCT method, and against a standard curve. Digital drop PCR was also used to calculate expression ratios. Student's t test and linear regression methods examined possible differences between genotypes. During 32 runs (10 replicates each), the RT-qPCR mean (SD) CRP/GAPDH ratio was 3.39 × 10–4 (SD 1.73 × 10–4) and 3.15 × 10–4 (SD 1.64 × 10–4) for TT and CC genotypes respectively, p = 0.76; and digital drop PCR results were similar. Serum CRP was not significantly different between genotypes, nor correlated with leukocyte expression. CRP is minimally expressed in unactivated leukocytes and this expression is not likely influenced by rs1205 genotype.
format	article
author	L. G. Best C. Azure K. Martell K. S. Tsosie B. Voels
author_facet	L. G. Best C. Azure K. Martell K. S. Tsosie B. Voels
author_sort	L. G. Best
title	Unactivated leukocyte expression of C-reactive protein is minimal and not dependent on rs1205 genotype
title_short	Unactivated leukocyte expression of C-reactive protein is minimal and not dependent on rs1205 genotype
title_full	Unactivated leukocyte expression of C-reactive protein is minimal and not dependent on rs1205 genotype
title_fullStr	Unactivated leukocyte expression of C-reactive protein is minimal and not dependent on rs1205 genotype
title_full_unstemmed	Unactivated leukocyte expression of C-reactive protein is minimal and not dependent on rs1205 genotype
title_sort	unactivated leukocyte expression of c-reactive protein is minimal and not dependent on rs1205 genotype
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ba583cfdc0244f0b916eaafced98f1a0
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Unactivated leukocyte expression of C-reactive protein is minimal and not dependent on rs1205 genotype

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